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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
ChIP-Seq inopa genome-wide profiling yeDNA tarisiro yekuchinja kwehistone, zvinyorwa zvinyorwa, uye mamwe mapuroteni ane DNA.Inosanganisa kusarudzwa kwechromatin immuno-precipitation (ChIP) kudzoreredza chaiwo mapuroteni-DNA complexes, nesimba reinotevera-chizvarwa sequencing (NGS) yekumusoro-kuburikidza kutevedzana kweiyo yakadzoserwa DNA.Pamusoro pezvo, nekuti maprotein-DNA complexes anodzoserwa kubva kumaseru mhenyu, nzvimbo dzinosunga dzinogona kuenzaniswa mumhando dzakasiyana dzesero uye tishu, kana pasi pemamiriro akasiyana.Zvikumbiro zvinotangira kubva pakunyorwa kwegwara kuenda kunzira dzekusimudzira kune nzira dzechirwere uye nekupfuura.
Platform: Illumina NovaSeq 6000
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Yese genome bisulfite kutevedzana
DNA methylation panzvimbo yechishanu mu cytosine (5-mC) ine simba guru pakutaura kwemajini uye kushandiswa kwemasero.Abnormal methylation maitiro akave akabatanidzwa neanoverengeka mamiriro uye zvirwere, senge kenza.WGBS yave chiyero chegoridhe chekudzidza genome-wide methylation pane imwechete base resolution.
Platform: Illumina NovaSeq6000
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Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira throughput Sequencing (ATAC-seq)
ATAC-seq inzira yepamusoro-yekutevedzana yekuongorora genome-wide chromatin accessibility, iyo yakakosha kupasi rose epigenetic control ye gene expression.Sequencing adapters inoiswa munzvimbo dzakavhurika chromatin ne hyperactive Tn5 transposase.Mushure mePCR kukwidziridzwa, raibhurari yekutevedza inogadzirwa.Ese akavhurika chromatin matunhu anogona kuwanikwa pasi peiyo nzvimbo-yenguva mamiriro, kwete chete inogumira kune inosunga masaiti echinyorwa chinhu, kana imwe histone yakagadziridzwa dunhu.
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Yakaderedzwa Representation Bisulfite Sequencing (RRBS)
Tsvagiridzo yeDNA methylation yagara iri nyaya inopisa mukutsvaga kwechirwere, uye yakanyatsoenderana nekutaura kwemajini uye pheno-typic maitiro.RRBS inzira yakarurama, inoshanda uye ine mari yeDNA methylation research.Kufumiswa kweanosimudzira uye CpG matunhu ezvitsuwa ne enzymatic cleavage (Msp I), yakasanganiswa neBisulfite sequencing, inopa yakakwirira resolution DNA methylation yekuona.
Platform: Illumina NovaSeq 6000
