Yakazara-urefu mrna sequencing-nanopore
Zvimiro
● Kurwa kwePoly-a MrNa vakateverwa neCDNA SYNTNSISS uye Raibhurari Kugadzirira
● Kuongorora kwemanyoro ezere-akareba
● Bioinformatic Ongororo Yakavakirwa Pazvinongement kune Reference
● Bioinformatic Ongororo Inosanganisira chete kutaura kuGene uye isealorm-levels asiwo kuongorora kweLNCRAN, Poly-Adency
Zvigadzirwa zvebasa
●Kuwanda kwekutaura kuoctoroform level.
●Yakaderedzwa data inoda:Kuenzaniswa neyekupedzisira
●Kurongeka kwepamusoro kwekutaura kwehuwandu: Ose ari maviri paGene uye Oshomal level
●Kuzivikanwa kweruzivo rwekuwedzera mutsara: imwe polyadenylation, fiusion majini uye lcnrna uye yavo jekiseni
●Hunyanzvi hwakawanda.
●Post-Kutengesa Tsigiro: Kuzvipira kwedu kunowedzera kupfuura projekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa nguva yebasa. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
Mhedzisiro Inodiwa uye Kuendesa
| Raibhurari | Kuongorora zano | Data yakakurudzirwa | Mhando yekudzora |
| Poly yakafumiswa | Illumina Pe150 | 6/12 GB | Avhareji yemhando yepamusoro: Q10 |
Zvinodiwa Muenzaniso:
Nucleotide:
| Conc. (Ng / μl) | Mari (μg) | Kuchena | Kuvimbika |
| ≥ 100 | ≥ 1.0 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Limited kana hapana protein kana kusvibiswa kweDNA kunoratidzwa pane gel. | Zvidyo: Rin≥7.0; Zvemhuka: Rin≥7.5; 5.0≥28s / 18s≥1.0; Limited kana Hapana Baseline kukwirira |
● Zvirimwa:
Midzi, tsinde kana petal: 450 mg
Shizha kana Mbeu: 300 mg
Michero: 1.2 g
● Mhuka:
Moyo kana mudumbu: 300 mg
Viscera kana uropi: 240 mg
Muswe: 450 mg
Mapfupa, bvudzi kana ganda: 1g
● Arthropood:
Zvipembenene: 6g
Crustacea: 300 mg
● Ropa rese: 1 tube
● Masero: 106 masero
Yakakurudzirwa Samplumple Kutumira
Mudziyo: 2 ml centrifige tube (tin foil haina kukurudzirwa)
Sampula Inonyora: Boka + inodzokorora semuenzaniso A1, A2, A3; B1, B2, B3.
Kutumira:
1. Dry-Ice: samples dzinoda kutariswa mumabhegi uye kuvigwa mune yakaoma-ice.
2..
Basa rebasa rinoyerera
Nucleotide:
Muenzaniso
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Basa rebasa rinoyerera
Tishu:
Kuedza Dhizaini
Muenzaniso
Rna kubviswa
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
● Raw Data Kugadzirisa
● Kuzivikanwa kweNyanzvi
● Imwe nzira yekunyepedzera
● Kugadziriswa kwezasi mune gene level uye isoform level
● Kusiyanisa kutaura kwakasiyana
● Basa resumo uye nekuvandudza (degs uye dests)
Zvimwe zvinotsemuka kuongorora
Imwe nzira Polyadenylation Ongororo (APA)
lncrona kufanotaura
Anotaurwa weCovel Jesve
Kubatanidzwa kwe crets
Protein-Protein Networks muDGS
Ongorora kufambira mberi kwakabatanidzwa neBMKGE aN's NAPOPORE yakazara MrN
Gong, b. et al. . Doi: 10.1016 / J.Kgg.2023.01.008.
Iye, Z. Et Al. . 108636. DOI: 10.1016 / j.fsi.2023.108636.
Ma, y. Et al. . 110709. DOI: 10.1016 / J.ygeno.2023.110709.
Yu, D. et al. . Doi: 10.1186 / S13287-023-03491-5 / matafura / 6.
