Kuongorora Kubatana kweGenome-wide
Kufamba kwebasa
Zvakanakira Basa
●Unyanzvi Hwakanyanya uye zvinyorwa zvekubudisa: neruzivo rwakawanda muGWAS, BMKGene yakapedza mazana emapurojekiti emhando dzemhuka mukutsvagisa kwevanhu muGWAS, yakabatsira vaongorori kuburitsa zvinyorwa zvinopfuura zana, uye huwandu hwemhedzisiro hwakasvika mazana mashanu.
● Kuongorora kwakazara kweruzivo rwezvinhu zvipenyu: mafambiro ebasa anosanganisira ongororo yekubatana kweSNP-trait, ichipa seti yemajini akakodzera uye functional annotation yavo inoenderana.
●Chikwata chine hunyanzvi hwepamusoro mu bioinformatics uye ongororo pfupi: neruzivo rwakakura mukuongorora ma genomics epamusoro, timu yeBMKGene inopa ongororo yakazara nekukurumidza.
●Rutsigiro rweMushure meKutengesa:Kuzvipira kwedu kunopfuura kupedzwa kweprojekiti nemwedzi mitatu yebasa mushure mekutengesa. Munguva ino, tinopa rubatsiro rwekutevera purojekiti, rubatsiro rwekugadzirisa matambudziko, uye mibvunzo nemhinduro kuti tipindure chero mibvunzo ine chekuita nemhedzisiro.
Zvinodiwa nebasa
| Rudzi rwekutevedzana | Chiyero chehuwandu hwevanhu chinokurudzirwa | Maitiro ekuteverana | Zvinodiwa zveNucleotide |
| Kutevedzana Kwemajini Ese | sampuro mazana maviri | 10x | Kuwanda kwemvura: ≥ 1 ng/ µL Huwandu hwese ≥ 30ng Kuora kana kusvibiswa zvishoma kana kusavapo |
| Chidimbu Chinopamhidzirwa (SLAF) | Kudzika kweTag: 10x Huwandu hwema tagi: <400 Mb: WGS inokurudzirwa < 1Gb: 100K tags 1Gb > 2Gb: 300K tag Ma tag epamusoro e500k | Kuwanda kwemvura ≥ 5 ng/µL Huwandu hwese ≥ 80 ng Gel yeAgarose: haina kana kuti haina kusvibiswa kana kusvibiswa kushoma
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Kusarudzwa Kwezvinhu
Mhando dzakasiyana dzemhuka, mhando dzemhuka, minda/mabhangi emumagene/mhuri dzakasanganiswa/zviwanikwa zvemusango
Mhando dzakasiyana dzemhuka, subspecies, landraces
Mhuri yehama yepedyo/mhuri yehama yepedyo/zviwanikwa zvemusango
Kufamba Kwebasa Rebasa
Dhizaini yekuedza
Kutumirwa kwemuenzaniso
Kubviswa kweRNA
Kuvakwa kweraibhurari
Kutevedzana kwezvikamu
Kuongorora data
Masevhisi ekupedzisira ekutengesa
Inosanganisira ongororo inotevera:
- Kuongorora kubatana kwemajini ese: LM, LMM, EMMAX, FASTLMM modhi
- Tsanangudzo inoshanda yemajini anodiwa
Ongororo yekubatana kwehunhu hweSNP - Manhattan plot
Ongororo yekubatana kwehunhu hweSNP - QQ plot
Ongorora kufambira mberi kuri kuitwa nemabasa eBMKGene's de GWAS kuburikidza nemuunganidzwa wezvinyorwa zvakarongwa:
Lv, L. et al. (2023) 'Kunzwisisa nezvehwaro hwemajini hwekushivirira ammonia mu razor clam Sinonovacula constricta kuburikidza nekudzidza kwekubatana kwegenome',Kurima gungwa, 569, peji 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
Li, X. nevamwe (2022) 'Kuongororwa kwemhando dzakasiyana dzemaomiki dze398 foxtail millet kunoratidza nzvimbo dzemajini dzine chekuita nekuchengetwa kwemhuka, hunhu hwemetabolite, uye mhedzisiro yekudzivirira kuzvimba',Chirimwa cheMolecular, 15(8), mapeji 1367–1383. doi: 10.1016/j.molp.2022.07.003.
Li, J. nevamwe (2022) 'Kugadzira Mapu eGenome-Wide Association of Hulless Barely Phenotypes in Drought Environment',Miganhu muSainzi yeZvirimwa, 13, peji 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. nevamwe (2021) 'GmST1, iyo inochengetedza sulfotransferase, inopa simba rekudzivirira hutachiona hwesoya mosaic G2 neG3',Chirimwa, Sero & Nzvimbo Yakatipoteredza, 44(8), mapeji 2777–2792. doi: 10.1111/PCE.14066.
