Yakareba isiri-coding sequencing-Illumina
Zvigadzirwa zvebasa
●Kuongorora kwekunyora kwa MrNN naLncrna.
●Hunyanzvi hwakawanda.
●Rudzius mhando yekudzoraIzvi Uku kutarisisa kwekutarisa kunovimbisa kuburitsa kwemhedzisiro yemhando yepamusoro yepamusoro.
●Post-Kutengesa Tsigiro: Kuzvipira kwedu kunowedzera kupfuura projekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa nguva yebasa. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
Mhedzisiro Inodiwa uye Kuendesa
| Raibhurari | Chikuva | Yakakurudzirwa data | Data qc |
| rrna yakadzikira raibhurari raibhurari | Illumina Pe150 | 10-16 GB | Q3085% |
Nucleotide:
| Conc. (Ng / μl) | Mari (μg) | Kuchena | Kuvimbika |
| ≥ 80 | ≥ 0.8 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Limited kana hapana protein kana kusvibiswa kweDNA kunoratidzwa pane gel. | RINO6.0; 5.0≥28s / 18s≥1.0; Limited kana Hapana Baseline kukwirira |
● Zvirimwa:
Midzi, tsinde kana petal: 450 mg
Shizha kana Mbeu: 300 mg
Michero: 1.2 g
● Mhuka:
Moyo kana mudumbu: 450 mg
Viscera kana uropi: 240 mg
Muswe: 600 mg
Mapfupa, bvudzi kana ganda: 1.5g
● Arthropood:
Zvipembenene: 9g
Crustacea: 450 mg
● Ropa rese:2 machubhu
● Masero: 106 masero
● Serum uye plasma: 6 ml
Yakakurudzirwa Samplumple Kutumira
Mudziyo: 2 ml centrifige tube (tin foil haina kukurudzirwa)
Sampula Inonyora: Boka + inodzokorora semuenzaniso A1, A2, A3; B1, B2, B3.
Kutumira:
1. Dry-Ice: samples dzinoda kutariswa mumabhegi uye kuvigwa mune yakaoma-ice.
2..
Basa rebasa rinoyerera
Kuedza Dhizaini
Muenzaniso
Rna kubviswa
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Bioinformatics
Kutaura kwakasiyana kweGene (DEGS) kuongororwa
Kurehwa kweLncrna Expression - Inonamatira
Kuwedzeredza kweLNCRAT TARITE JENY
Joint mrN
Ongorora kufambira mberi kwekufambisa neBMKGE aLNCRACE's LNCNING Sevhisi kuburikidza nekuunganidzwa kwakasarudzika kwemabhuku.
Ji, H. Et Al. . Doi: 10.1038 / S41598-0257451-7.
Jia, z. et al. . 687151. DOI: 10.3389 / fimmu.2021.687151 / Bibtex.
Wang, xj et al. . 904865. Doi: 10.3389 / Fanc.2022.904865 / Bibtex.
Xiao, l. et al. . Doi: 10.1111 / PBI.13270.
Zheng, H. Et Al. . " 879824. Doi: 10.3389 / fimmu.2022.879824 / Bibtex.
