Chirimwa/Mhuka Yese Genome Sequencing

Yese genome re-sequencing, inozivikanwawo seWGS, inogonesa kuburitswa kwezvose zvakajairika uye zvisingawanzo shanduko pajenome rese kusanganisira Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), uye Copy Number Variation (CNV). )SVs inogadzira chikamu chikuru chekusiyana kwekusiyana kweSNPs uye ine simba guru pane genome, iyo ine simba guru pane zvipenyu.Kuverengera-kurebesa requencing kunobvumira kunyatsozivikanwa kwezvimedu zvakakura uye misiyano yakaoma nekuti kuverenga kwenguva refu kunoita kuti zvive nyore kuyambuka chromosomal kuyambuka matunhu akaoma senge tandem kudzokorora, GC / AT-akapfuma matunhu, uye hyper-anosiyana matunhu.

Platform: Illumina, PacBio, Nanopore

Taura nesu

Service Details

Demo Result

Featured Publication

1.Service Advantages

Zvakawanda ruzivo mukutevedzana kwegenome kune anopfuura 1000 marudzi.

Pamusoro pe800 akaburitswa makesi ane accumulative impact factor inopfuura 4000.

Yakakwana bioinformatics ongororo pane musiyano wekufona uye basa rekuongorora.

2. Zvinyorwa zveSevhisi

Platform		Library	Inokurudzirwa seq kudzika
Illumina		PE150	YeSNP, InDel inofona ≥ 10x YeSV, CNY ichifona ≥ 30x
Nanopore		8 kb	YeSV, CNY inofona ≥ 20x
Pacbio	CCS	15 kb	YeSNP, InDel, SV, CNY inofona ≥ 10x

3. Sample Zvinodiwa

Platform		Conc.(ng/μL)	Mari (ng)	Kuchena		Agarose gel
				OD260/280	OD260/230	1. Bvisa bhendi guru risina kana muganhukuora kunoonekwa pa gel. 2. Kwete kana kuti shoma RNA kana mapuroteni kusvibiswa
Illumina		≥1	≥30	-	-
Nanopore		≥30	Zvinoenderana negoho re data 10μg/sero	1.7-2.2	≥1.5
Pacbio	CCS	≥50		1.7-2.2	1.8-2.5

4. Bioinformation Analysis

5. Kuyerera Kwebasa Rebasa

Muenzaniso wekutumira

Kutorwa kweDNA

Kuvakwa kweraibhurari

Sequencing

Data analysis

Kuendesa data

Zvakapfuura: BMKMANU S1000 Spatial Transcriptome

Zvinotevera: Single-nucleus RNA Sequencing

1) Nhamba dzeGenome Mapping

Table 1 Statistics yemepu mhinduro

Mufananidzo 1 Kugovera kwehukuru hwekuisa uye kuverenga kuvharwa.

2) Kusiyanisa Kuonekwa

Mufananidzo 2 Nhamba uye chirevo cheSNP/INDEL/SV pakati peSamples

Mufananidzo 3 Genome-yakapararira kugoverwa kweatiations

3) Kushanda tsinhiro yekusiyana

2019	Kutaurirana kwakasikwa	Yese-genome resequencing inoratidza Brassica napus mavambo uye genetic loci inobatanidzwa mukuvandudza kwayo
2020	PNAS	Iyo yekushanduka-shanduka uye nhoroondo yekuchengetwa kwegoldfish (Carassius auratus)
2021	Plant Biotechnology Journal	Reference genomes emhando mbiri dzakarimwa dzejute
2021	Plant Biotechnology Journal	Genomic siginicha yemuriwo neoiri allopolyploid Brassicajuncea uye genetic loci inodzora kuunganidzwa kweglucosinolates.
2019	Molecular Plant	Yese-genome requencing yepasi rose muunganidzwa wezvakabatwa chibharo zvinoburitsa genetic hwaro hwekusiyana kwavo ecotype.
2022	Horticulture Research	Kuongororwa kweGenome-wide association kunopa ma molecular nzwisiso mukusiyana kwechisikigo kwekukura kwembeu yemavito
2021	Chinyorwa cheKuedza Botany	Genome-wide association kudzidza inozivisa mutsauko weGhSAD1 inopa kushivirira kutonhora mudonje.
2021	Chinyorwa cheKuedza Botany	Genome-wide association kudzidza uye transcriptome kuenzanisa kunoratidza novel QTL uye mumiriri majini anodzora petal size mune yakabatwa chibharo.