Prokaryotic RNA kutevedzana

Prokaryotic RNA sequencing Featured Image

Prokaryotic RNA sequencing inoshandisa chizvarwa chinotevera kutevedzana (NGS) kuratidza huvepo uye huwandu hweRNA panguva yakapihwa, nekuongorora shanduko yemaserura transcriptome.Kambani yedu prokaryotic RNA sequencing, yakanangana nemaprokaryotes ane referensi genomes, ichikupa transcriptome profiling, geni chimiro chekuongorora, nezvimwe. Yakashandiswa zvakanyanya kutsvagurudzo yesainzi, tsvakiridzo yezvinodhaka nekusimudzira, nezvimwe.

Platform: Illumina NovaSeq Platform

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Service Details

Sevhisi Zvinotsanangurwa

Platform: Illumina NovaSeq chikuva, PE150

Rudzi rweraibhurari: 350bp isa cDNA raibhurari (zvinoenderana nekuparadzirwa kwepamusoro)

Sequencing zano: Paired-End 150 bp

Kurudzira huwandu hwe data: 2 Gb mbishi data/sample

Bioinformatics

(1) Sequencing data quality control: Nucleotide distribution, Base quality distribution, rRNA ratio assessment;

(2) Sequence alignment analysis: Alignment result statistics, Saturation of sequencing, Coverage of sequencing;

(3) Reference genome annotation (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Tsanangudzo yekutaura: Kuparidzirwa kwekutaura (Nezvinyorwa zviviri kana kupfuura), Kuongorora kwekutaura pakati pemasampuli (Venn analysis, Correlation analysis, PCA analysis);

(5) Differential expression analysis (Nemasampuli maviri kana anopfuura);

(6) Gene set analysis: Venn analysis, Cluster analysis, Function annotation analysis (COG, GO, KEGG), Basa rekusimudzira kuongorora (GO, KEGG), Basa rekusimudzira chordal graph, Ipath analysis;

(7) sRNA kuongorora: sRNA kufanotaura, sRNA annotation (Blast, sRNAMAp, sRNATarBase, SIPHT, Rfam), sRNA yechipiri chimiro kufanotaura, sRNA chinangwa chegene kufanotaura;

(8) Kuongororwa kwemaitiro ezvinyorwa: Operon analysis, Transcription kutanga uye kupedzisa nzvimbo dzekufanotaura, UTR annotation uye kuongorora, Promoter sequence prediction, SNP / InDel kuongorora (SNP / InDel annotation, SNP / InDel kugoverwa kwenharaunda, SNP statistics).