Yakaderedzwa Representation Bisulfite Sequencing (RRBS)
Sevhisi Zvinotsanangurwa
Platform: NovaSeq chikuva, PE150
Raibhurari mhando: 200-400bp isa bisulfite yakarapwa DNA raibhurari
Sequencing zano: Paired-End 150 bp
Kurudzira kuburitsa data: 10 Gb mbishi data/sample
Muenzaniso Zvinodiwa
DNA yakawanda: ≥ 2ug
DNA concentration:≥ 20ng/μl.
Kuchena: OD260/280 = 1.8 kusvika 2.0 pasina kuora kana kusvibiswa kweRNA
Bioinformatics analysis
Kuyerera Kwebasa Rebasa
Muenzaniso wekutumira
Kuvakwa kweraibhurari
Sequencing
Data analysis
Mushure mekutengesa masevhisi
a.Reference genome alignment statistics
Nhamba dzemapu dzinoverengwa
Statistics yekuteedzera kudzika uye kufukidzwa kweC saiti
Ayakaunganidzwa
Digestion kunyatsoshanda kwemuenzaniso wega wega
b.Methylation nzvimbo yekuona
Rondedzero yemazinga emethylation paC saiti
BS kutendeuka
Methylated cytosine
Methylation level kugovera
c.Methylation mepu
Genome-wide
Genome element
d.Kuenzanisa kwemazinga emethylation pakati pemasampuli
PAirwise Correlation yeCG methylation
PCA-CG methylation
Ckupenya kweCG methylation
d.Yakasiyana methylation kuongororwa
Snhamba dzeDMR
DMR kureba kugoverwa
Hkudya mepu
DMR kugovera munzvimbo yakanangwa
