Single-nucleus RNA Sequencing

Iko kufambira mberi mukutora sero rimwe chete uye nzira yekuvaka raibhurari yega-inosanganiswa neyepamusoro-kuburikidza kutevedzana inobvumira gene expression zvidzidzo pasero-ne-sero.Iyo inogonesa yakadzama uye yakazara sisitimu yekuongorora pane yakaoma cell populations, iyo inodzivirira zvakanyanya masking yehterogeneity yavo nekutora mavhareji emasero ese.

Zvisinei, mamwe masero haana kukodzera kuitwa mu-single-sero suspension, saka dzimwe nzira dzekugadzirira sampuli - nucleus extraction kubva kumatissue inodiwa, kureva kuti, nucleus inotorwa zvakananga kubva mumatishu kana sero uye inogadzirirwa mu-single-nucleus suspension for single- cell sequencing.

BMK inopa gumi × Genomics Chromium TM yakavakirwa imwe-sero RNA sequencing sevhisi.Iyi sevhisi yakashandiswa zvakanyanya muzvidzidzo pazvidzidzo zvine chekuita nechirwere, senge immune cell kusiyanisa, tumor heterogeneity, kukura kwetishu, nezvimwe.

Spatial transcriptome chip: 10 × Genomics

Platform: Illumina NovaSeq Platform

Taura nesu

Service Details

Bioinformatics

Demo Results

Technical musimboti

Kuparadzaniswa kweiyo nuclei kunowanikwa ne10 × Genomics Chromium TM, iyo ine masere-channel microfluidics system ine kaviri kuyambuka.Mune ino system, gel mabheji ane barcode uye primer, enzymes uye imwe nucleus yakavharirwa mu nanoliter-saizi mafuta donhwe, kugadzira Gel Bead-in-Emulsion (GEM).Kana GEM ichinge yaumbwa, sero lysis uye kuburitswa kwebarcode kunoitwa muGEM yega yega.mRNA inodzokororwa yakanyorwa kuita cDNA mamorekuru ane 10 × barcode uye UMI, ayo ari pasi peyakajairwa kuteedzera raibhurari kuvaka.

Tissue haikodzeri kugadzirira kwesero rimwe chete kumiswa

Sero / Tishu	Chikonzero
Matishu asina kutsva nechando	Kutadza kuwana masangano matsva kana akachengetwa kwenguva refu
Tsandanyama sero, Megakaryocyte, Mafuta…	Sero dhayamita rakakurisa kupinda chiridzwa
Chiropa…	Yakanyanya kuputsika, haikwanise kusiyanisa masero ega
Neuron cell, Brain…	Kunyanya kunzwira, nyore kushushikana, kuchachinja kutevedzana kwemhedzisiro
Pancreas, Thyroid ...	Yakapfuma mune endogenous enzymes, inokanganisa kugadzirwa kwesero rimwe chete kumiswa

Single-nucleus vs Single-sero

Single-nucleus	Single-sero
Unlimited sero dhayamita	Sero dhayamita: 10-40 μm
Nyaya yacho inogona kunge yakaomeswa nechando	Izvo zvinhu zvinofanirwa kunge zviri zvinyoro zvitsva
Kuderera kuderera kwemasero akaomeswa nechando	Kurapa kwe enzyme kunogona kukonzera kushushikana kwesero
Hapana masero matsvuku eropa anofanira kubviswa	Masero matsvuku eropa anofanira kubviswa
Nuclear inotaura bioinformation	Sero rese rinoburitsa bioinformation