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Diki RNA sequencing-Illumina

Madiki eRNA (sRNA) mamorekuru, anowanzo pasi pe200 nucleotides pakureba, anosanganisira microRNAs (miRNAs), maRNA madiki anopindira (siRNAs), uye piwi-interacting RNAs (piRNAs).Pakati peaya, miRNAs, yakatenderedza 20-24 nucleotides kureba, inonyanya kukosha pamabasa avo akakosha ekutonga mumaserura akasiyana maitiro.Nematishu-chaiwo uye nhanho-chaiwo maitiro ekutaura, miRNAs inoratidza kuchengetedza kwepamusoro pamarudzi akasiyana.

Platform: Illumina NovaSeq


Service Details

Bioinformatics

Demo Results

Featured Publications

Features

● Kukura kusarudzwa kweRNA kusati kwagadzirira raibhurari

● Ongororo yeBioinformatic yakanangana nekufembera kwe miRNA uye zvinangwa zvavo

Basa Rinobatsira

Comprehensive bioinformatics analysis:kugonesa kuzivikanwa kwezvose zvinozivikanwa uye novel miRNAs, kuzivikanwa kwemiRNAs tarisiro uye inoenderana inoshanda annotation uye kupfumisa neakawanda dhatabhesi (KEGG, GO)

Rigorous Quality Control: Isu tinoshandisa epakati kudzora mapoinzi pamatanho ese, kubva kumuenzaniso uye kugadzirira raibhurari kune kutevedzana uye bioinformatics.Uku kutarisisa kwakadzama kunovimbisa kuburitswa kwezvinogara zvemhando yepamusoro mhedzisiro.

Post-Sales Support: Kuzvipira kwedu kunowedzera kupfuura kupera kweprojekiti nemwedzi mitatu mushure mekutengesa sevhisi nguva.Munguva ino, tinopa kutevedza kweprojekiti, rubatsiro rwekugadzirisa matambudziko, uye maQ&A masesheni kugadzirisa chero mibvunzo ine chekuita nemhedzisiro.

Hunyanzvi Hwakawanda: iine rekodhi rekodhi yekuvhara zvakabudirira pamusoro peakawanda sRNA mapurojekiti anofukidza anopfuura zana emarudzi munzvimbo dzakasiyana dzekutsvagisa, timu yedu inounza hupfumi hweruzivo kupurojekiti yega yega.

Sample Zvinodiwa uye Delivery

Library

Platform

Yakakurudzirwa data

Data QC

Saizi yakasarudzwa

Illumina SE50

10M-20M inoverengwa

Q30≥85%

Sample Zvinodiwa:

Nucleotides:

Conc.(ng/μl)

Mari (μg)

Kuchena

Kuperera

≥ 80

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Yakaganhurirwa kana isina mapuroteni kana kusvibiswa kweDNA kunoratidzwa pagel.

RIN≥6.5;

5.0≥28S/18S≥1.0;

kukwirira kwakadzika kana kusina kwekutanga

● Zvirimwa:

Mudzi, Dzinde kana Petal: 450 mg

Shizha kana Mbeu: 300 mg

Muchero: 1.2 g

● Mhuka:

Mwoyo kana Ura: 450 mg

Viscera kana Brain: 240 mg

Tsandanyama: 600 mg

Mabhonzo, Bvudzi kana Ganda: 1.5g

● Arthropods:

Zvipembenene: 9g

Crustacea: 450 mg

● Ropa rose: 2 machubhu

● Masero: 106 masero

● Serum uye Plasma:6 mL

Yakakurudzirwa Sample Delivery

Container:
2 ml centrifuge chubhu (Tin foil haina kukurudzirwa)
Sample labeling: Boka+ replicate eg A1, A2, A3;B1, B2, B3...

Shipment:
1.Dry-ice: Samples dzinoda kuiswa mumabhegi uye kuvigwa muoma-ice.
2.RNAstable tubes: RNA samples inogona kuomeswa muRNA stabilization tube (eg RNAstable®) uye inotumirwa mukupisa kwekamuri.

Kuyerera Kwebasa Rebasa

Muenzaniso QC

Kuedza kugadzira

sampuli yekuendesa

Muenzaniso wekutumira

Pilot experiment

RNA kubviswa

Kugadzirira Raibhurari

Kuvakwa kweraibhurari

Sequencing

Sequencing

Data analysis

Data analysis

Mushure mekutengesa Services

Mushure mekutengesa masevhisi


  • Zvakapfuura:
  • Zvinotevera:

  • Bioinformatics

    wps_doc_14

    Kuzivikanwa kwe miRNA: chimiro uye kudzika

     

     

     miRNA-precursor-chimiro-uye-kutevedzana-kudzika

     

    Kusiyana kwekutaura kwe miRNA - hiearchical clustering

    图片34

     

    Inoshanda tsinhiro yechinangwa chemiRNA yakaratidzwa zvakasiyana

    图片35

    Ongorora kufambira mberi kwekutsvagisa kunofambiswa neBMKGene 'sRNA kutevedzana masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.

      

    Chen, H. et al.(2023) 'Zvirwere zvehutachiona zvinodzivisa saponin biosynthesis uye photosynthesis muPanax notoginseng', Plant Physiology uye Biochemistry, 203, p.108038. doi: 10.1016/J.PLAPHY.2023.108038.

    Li, H. et al.(2023) 'Chirimwa FYVE domain-ine protein FREE1 inoshamwaridzana nemicroprocessor zvikamu kudzvinyirira miRNA biogenesis', EMBO inoshuma, 24 (1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.

    Yu, J. nevamwe.(2023) 'The MicroRNA Ame-Bantam-3p Inodzora Larval Pupal Development neTargeting the Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) muHoneybee, Apis mellifera', International Journal of Molecular Sciences, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.

    Zhang, M. et al.(2018) 'Integrated Analysis yeMiRNA uye Genes Inobatanidza Nehutano Hwenyama Inoratidza kuti Gga-MiR-140-5p Inobata Intramuscular Fat Deposition muHuku', Cellular Physiology uye Biochemistry, 46 (6), mapeji 2421-2433.doi: 10.1159/000489649.

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