Diki RNA Sequencing-Illumina
Features
● Kugadzirira raibhurari yekusarudza saizi.
● Ongororo yeBioinformatic yakanangana nekufembera kwe miRNA uye zvinangwa.
Zvakanakira Basa
●Hunyanzvi Hwakawanda: Isu takagadzirisa anopfuura mazana matatu emhando, anotora akasiyana emhando dzemhando. Isu tinounza hupfumi hwehunyanzvi kupurojekiti yega yega.
●Rigorous Quality Control: Isu tinoshandisa epakati kudzora mapoinzi pamatanho ese, kubva pakugadzirira sampuli kusvika kukugadzirira raibhurari, kutevedzana uye bioinformatics. Kutarisisa kwedu kwehungwaru kunovimbisa kuburitswa kwezvinogara zvemhando yepamusoro mhedzisiro.
●Comprehensive bioinformatics analysis:Inogonesa kuzivikanwa kwezvose zvinozivikanwa uye novel miRNAs, kuzivikanwa kwemiRNAs tarisiro, uye inoenderana inoshanda tsinhiro uye kupfumisa neakawanda dhatabhesi (KEGG, GO).
●Post-Sales Support: Tinonzwisisa kukosha kwakakosha kuvepo, ndosaka kuzvipira kwedu kuchipfuura kupera kweprojekiti nemwedzi mitatu mushure mekutengesa sevhisi. Munguva ino, tinopa kutevedza kweprojekiti, rubatsiro rwekugadzirisa matambudziko, uye maQ&A masesheni kugadzirisa chero mibvunzo ine chekuita nemhedzisiro.
Sample Zvinodiwa uye Delivery
| Library | Platform | Yakakurudzirwa data | Data QC |
| Saizi yakasarudzwa | Illumina SE50 | 10M-20M inoverengwa | Q30≥85% |
Sample Zvinodiwa:
Nucleotides:
| Conc.(ng/μl) | Mari (μg) | Kuchena | Kuperera |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Yakaganhurirwa kana isina mapuroteni kana kusvibiswa kweDNA kunoratidzwa pagel. | RIN≥6.0; 5.0≥28S/18S≥1.0; kukwirira kwakadzika kana kusina kwekutanga |
● Zvirimwa:
Mudzi, Dzinde kana Petal: 450 mg
Shizha kana Mbeu: 300 mg
Muchero: 1.2 g
● Mhuka:
Mwoyo kana Ura: 450 mg
Viscera kana Brain: 240 mg
Tsandanyama: 600 mg
Mabhonzo, Bvudzi kana Ganda: 1.5g
● Arthropods:
Zvipembenene: 9g
Crustacea: 450 mg
● Ropa rose: 2 machubhu
● Masero: 106 masero
● Serum uye Plasma:6 mL
Yakakurudzirwa Sample Delivery
Container: 2 ml centrifuge chubhu (Tin foil haina kukurudzirwa)
Sample labeling: Boka+ replicate eg A1, A2, A3; B1, B2, B3.
Shipment:
1. Dry-ice: Samples dzinoda kurongedzerwa mumabhegi uye kuvigwa muoma-aizi.
2. RNAstable tubes: RNA samples inogona kuomeswa muRNA stabilization tube (eg RNAstable®) uye inotumirwa mukamuri yekupisa.
Kuyerera Kwebasa Rebasa
Kuedza kugadzira
Muenzaniso wekutumira
RNA kubviswa
Kuvakwa kweraibhurari
Sequencing
Data analysis
Mushure mekutengesa masevhisi
Bioinformatics
● Raw data quality control
● Chikamu chiduku cheRNA
● miRNA kutaura uye kusiyanisa kutaura kuongorora
● miRNA target gene uye yakaratidza zvakasiyana miRNA target gene annotation
● Target gene annotation uye zvakasiyana-siyana yakaratidza miRNA gene basa rekusimudzira
Kuzivikanwa kwe miRNA: chimiro uye kudzika
Kusiyana kwekutaura kwe miRNA - hiearchical clustering
Inoshanda tsinhiro yechinangwa chemiRNA yakaratidzwa zvakasiyana
Ongorora kufambira mberi kwekutsvagisa kunofambiswa neBMKGene 'sRNA kutevedzana masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.
Chen, H. et al. (2023) 'Zvirwere zvehutachiona zvinodzivisa saponin biosynthesis uye photosynthesis muPanax notoginseng',Plant Physiology uye Biochemistry, 203, p. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) 'Chirimwa FYVE domain-ine protein FREE1 inoshamwaridzana ne microprocessor zvikamu kudzvinyirira miRNA biogenesis',EMBO inoshuma, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. nevamwe. (2023) 'MicroRNA Ame-Bantam-3p Inodzora Larval Pupal Development neTargeting the Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) muHoneybee, Apis mellifera',International Journal yeMolecular Sayenzi, 24(6), p. 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al. (2018) 'Integrated Analysis yeMiRNA uye Genes Inobatanidzwa neNyama Hunhu Inozivisa kuti Gga-MiR-140-5p Inokanganisa Intramuscular Fat Deposition muHuku',Cellular Physiology uye Biochemistry, 46(6), mapeji 2421–2433. doi: 10.1159/000489649.
