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Yakazara-kureba mRNA sequencing-Nanopore
RNA sequencing yanga iri chishandiso chakakosha chekuongorora kwakadzama transcriptome.Pasina kupokana, yechinyakare-pfupi-yekuverenga kutevedzana yakawana yakawanda yakakosha budiriro muno.Zvakadaro, inowanzosangana nemipimo mune yakazara-kureba isoform kuzivikanwa, quantification, PCR bias.
Nanopore sequencing inozvisiyanisa kubva kune mamwe mapuratifomu ekutevedzana, pakuti nucleotides inoverengwa zvakananga pasina DNA synthesis uye inogadzira kuverenga kwenguva refu pamakumi e kilobases.Izvi zvinopa simba rakananga kuverenga-kunze kuyambuka yakazara-kureba zvinyorwa uye kugadzirisa zvinonetsa muisoform-level zvidzidzo.
Platform:Nanopore PromethION
Raibhurari:cDNA-PCR
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De novo Yakazara-kureba Transcriptome sequencing -PacBio
De novofull-length transcriptome sequencing, inozivikanwawo seDe novoIso-Seq inotora zvakanakira PacBio sequencer mukureba kwekuverenga, iyo inogonesa kutevedzana kweakazara-kureba cDNA mamorekuru pasina chero mabreak.Izvi zvinodzivirira zvachose chero zvikanganiso zvinogadzirwa mumatanho egungano ezvinyorwa uye zvinovaka unigene seti ine isoform-level resolution.Iyi unigene seti inopa ruzivo rwakasimba rwemajini se "reference genome" pa transcriptome-level.Uye zvakare, kusanganisa nechizvarwa chinotevera chekuteedzera data, iyi sevhisi inopa iyo chaiyo quantification yeisoform-level kutaura.
Platform: PacBio Sequel IIRaibhurari: SMRT bhero raibhurari -
Eukaryotic mRNA sequencing-Illumina
mRNA sequencing inogonesa profiling yeese mRNAs akanyorwa kubva kumaseru pasi pemamiriro chaiwo.Iyo tekinoroji ine simba yekuburitsa chimiro chejeni, zvimiro zvemajini uye mamorekuru emamwe maitiro ebhayoloji.Parizvino, kutevedzana kwemRNA kwave kushandiswa zvakanyanya mukutsvaga kwakakosha, kuongororwa kwekiriniki, kukura kwezvinodhaka, nezvimwe.
Platform: Illumina NovaSeq 6000
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Non-Reference based mRNA sequencing-Illumina
mRNA sequencing inotora inotevera-chizvarwa kutevedzana tekiniki (NGS) kutora mutumwa RNA(mRNA) fomu Eukaryote panguva chaiyo iyo mamwe mabasa akakosha ari kuita.Chinyorwa chakareba kwazvo chakanamirwa chakanzi 'Unigene' uye chakashandiswa sereferensi yekuongorora kunotevera, inova nzira inoshanda yekudzidza mamorekuru nemambure ekutonga emarudzi pasina kutaurwa.
Mushure mekubatanidza data retranscriptome uye unigene inoshanda annotation
(1) SNP kuongorora, SSR kuongorora, CDS kufanotaura uye gene chimiro chichafanorongwa.
(2) Quantification ye unigene kutaura mumuenzaniso wega wega uchaitwa.
(3) Unigenes akasiyaniswa pakati pemasamples (kana mapoka) anozowanikwa zvichibva pane unigene kutaura.
(4) Kubatanidza, chirevo chekushanda uye kuongorora kwekusimudzira kweunigenes inotsanangurwa zvakasiyana ichaitwa.
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Yakareba isiri-coding kutevedzana-Illumina
Marefu asina kukodha maRNA (lncRNAs) imhando yemamorekuru eRNA ane hurefu hunodarika 200 nt, ayo anoratidzirwa nekuderera zvakanyanya kugona kwekodha.LncRNA, senhengo yakakosha mune isiri-coding RNAs, inonyanya kuwanikwa munyucleus uye plasma.Iko kusimukira mukuteedzera tekinoroji uye bioinformatics inogonesa kuzivikanwa kweakawanda novel lncRNAs uye kubatanidza ayo ane biological mabasa.Huchapupu hunowedzera hunoratidza kuti lncRNA inobatanidzwa zvakanyanya mu epigenetic regulation, transcription regulation uye post-transcription regulation.
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Diki RNA kutevedzana-Illumina
Diki RNA inoreva kirasi yeasiri-coding RNA mamorekuru anowanzo asingasviki 200nt pakureba, anosanganisira micro RNA (miRNA), kupindira kudiki RNA (siRNA), uye piwi-inopindirana RNA (piRNA).
MicroRNA (miRNA) ikirasi ye endogenous diki RNA ine hurefu hunosvika 20-24nt, iyo inotamba akasiyana akakosha ekutonga mabasa mumaseru.miRNA inosanganisirwa mumaitiro mazhinji ehupenyu ayo anoburitsa matishu - chaiwo uye nhanho - chaiyo kutaura uye yakachengetedzwa zvakanyanya mumhando dzakasiyana.
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circRNA sequencing-Illumina
Yese transcriptome sequencing yakagadzirirwa kuratidza marudzi ese emamorekuru eRNA, anosanganisira coding (mRNA) uye asiri-coding RNAs (kusanganisira lncRNA, circRNA uye miRNA) ayo anonyorwa nemaseru chaiwo pane imwe nguva.Yese transcriptome sequencing, inozivikanwawo se "total RNA sequencing" ine chinangwa chekuburitsa akazara ekutonga network padanho rekunyora.Kutora mukana weNGS tekinoroji, kutevedzana kwezvese zvinyorwa zvinyorwa zviripo kune ceRNA ongororo uye yemubatanidzwa RNA kuongororwa, iyo inopa nhanho yekutanga kune inoshanda hunhu.Kuzivisa regulatory network ye circRNA-miRNA-mRNA yakavakirwa ceRNA.
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Yese transcriptome sequencing - Illumina
Yese transcriptome sequencing yakagadzirirwa kuratidza marudzi ese emamorekuru eRNA, anosanganisira coding (mRNA) uye asiri-coding RNAs (kusanganisira lncRNA, circRNA uye miRNA) ayo anonyorwa nemaseru chaiwo pane imwe nguva.Yese transcriptome sequencing, inozivikanwawo se "total RNA sequencing" ine chinangwa chekuburitsa akazara ekutonga network padanho rekunyora.Kutora mukana weNGS tekinoroji, kutevedzana kwezvese zvinyorwa zvinyorwa zviripo kune ceRNA ongororo uye yemubatanidzwa RNA kuongororwa, iyo inopa nhanho yekutanga kune inoshanda hunhu.Kuzivisa regulatory network ye circRNA-miRNA-mRNA yakavakirwa ceRNA.
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Prokaryotic RNA kutevedzana
Prokaryotic RNA sequencing inoshandisa chizvarwa chinotevera kutevedzana (NGS) kuratidza huvepo uye huwandu hweRNA panguva yakapihwa, nekuongorora shanduko yemaserura transcriptome.Kambani yedu prokaryotic RNA sequencing, yakanangana nemaprokaryotes ane referensi genomes, ichikupa transcriptome profiling, geni chimiro chekuongorora, nezvimwewo. Yakashandiswa zvakanyanya kutsvagurudzo yesainzi, tsvakiridzo yezvinodhaka nekusimudzira, nezvimwe.
Platform: Illumina NovaSeq 6000
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Metatranscriptome Sequencing
Metatranscriptome sequencing inozivisa gene expression yemicrobes (zvese eukaryotes uye prokaryotes) mukati mezvakatipoteredza (kureva ivhu, mvura, gungwa, tsvina, uye ura.) Kunyanya, masevhisi aya anobvumidza iwe kuwana yakazara gene kutaura profiling yenharaunda dzakaoma hutachiona, taxonomic analysis. yezvipenyu, ongororo yekusimudzira mashandiro emajini akasiyana anoratidzwa, nezvimwe.
Platform: Illumina NovaSeq 6000
