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Yese transcriptome sequencing - Illumina

Yese transcriptome sequencing inopa yakazara nzira yekunyora akasiyana mamorekuru eRNA, anosanganisira ese macoding (mRNA) uye asiri-coding RNAs (lncRNA, circRNA, uye miRNA).Iyi tekinoroji inobata iyo yakazara transcriptome yemaseru chaiwo panguva yakapihwa, ichibvumira kunzwisisa kwakazara kwemaserura maitiro.Iyo inozivikanwawo se "yakazara RNA kutevedzana," ine chinangwa chekufumura akaomesesa ekutonga network padanho rekunyora, ichigonesa kuongororwa kwakadzama sekukwikwidza endo native RNA (ceRNA) uye yemubatanidzwa RNA kuongororwa.Izvi zvinoratidzira danho rekutanga rekuita hunhu hwekuita, kunyanya mukufumura mataneti ekudzora anosanganisira circRNA-miRNA-mRNA-yakavakirwa ceRNA kusangana.


Service Details

Bioinformatics

Demo Results

Featured Publications

Features

● Dual raibhurari yekuteedzera iyo yakazara transcriptome: rRNA depletion inoteverwa nePE150 raibhurari kugadzirira uye saizi sarudzo inoteverwa neSE50 raibhurari kugadzirira.

● Zadzisa bioinformatics ongororo yemRNA, lncRNA, circRNA uye miRNA mune akasiyana bioinformatics mishumo.

● Kuongororwa kwakabatana kwese RNA kutaura mushumo yakasanganiswa, kusanganisira ceRNA network analysis.

Basa Rinobatsira

Kuongorora kwakadzama kwema network network: ceRNA network yekuongorora inogoneswa nekutevedzana kwakabatana kwemRNA, lncRNA, circRNA uye miRNA uye nekupedza bioinformatic workflow.

Comprehensive Annotation: Isu tinoshandisa akawanda dhatabhesi kuti tinyatso tsanangura iyo Differentially Inoratidzwa Genes (DEGs) uye kuita inoenderana ongororo yekuvandudza, ichipa ruzivo pane maserura uye mamorekuru maitiro ari pasi pemhinduro yezvinyorwa.

Hunyanzvi Hwakawanda: ine rekodhi rekodhi yekuvhara zvakabudirira pamusoro pe2000 yakazara mapurojekiti ezvinyorwa munzvimbo dzakasiyana siyana dzekutsvagisa, timu yedu inounza hupfumi hweruzivo kupurojekiti yega yega.

Rigorous Quality Control: Isu tinoshandisa epakati kudzora mapoinzi pamatanho ese, kubva kumuenzaniso uye kugadzirira raibhurari kune kutevedzana uye bioinformatics.Uku kutarisisa kwakadzama kunovimbisa kuburitswa kwezvinogara zvemhando yepamusoro mhedzisiro.

● Chirevo Chakakwana: Isu tinoshandisa akawanda dhatabhesi kuti tinyatso tsanangura iyo Differentially Inoratidzwa Genes (DEGs) uye kuita inoenderana ongororo yekuvandudza, ichipa ruzivo pane maserura uye mamorekuru maitiro ari pasi pemhinduro yezvinyorwa.

Post-Sales Support: Kuzvipira kwedu kunowedzera kupfuura kupera kweprojekiti nemwedzi mitatu mushure mekutengesa sevhisi nguva.Munguva ino, tinopa kutevedza kweprojekiti, rubatsiro rwekugadzirisa matambudziko, uye maQ&A masesheni kugadzirisa chero mibvunzo ine chekuita nemhedzisiro.

Sample Zvinodiwa uye Delivery

Library

Sequencing strategy

Data inokurudzirwa

Quality Control

rRNA yakapera

Illumina PE150

16 Gb

Q30≥85%

Saizi yakasarudzwa

Illumina SE50

10-20M inoverengwa

 

Sample Zvinodiwa:

Nucleotides:

Conc.(ng/μl)

Mari (μg)

Kuchena

Kuperera

≥ 100

≥ 1

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Yakaganhurirwa kana isina mapuroteni kana kusvibiswa kweDNA kunoratidzwa pagel.

Zvirimwa: RIN≥6.5

Mhuka: RIN≥7.0

5.0≥28S/18S≥1.0;

kukwirira kwakadzika kana kusina kwekutanga

Yakakurudzirwa Sample Delivery

Container:
2 ml centrifuge chubhu (Tin foil haina kukurudzirwa)
Sample labeling: Boka+ replicate eg A1, A2, A3;B1, B2, B3...

Shipment:
1.Dry-ice: Samples dzinoda kuiswa mumabhegi uye kuvigwa muoma-ice.
2.RNAstable tubes: RNA samples inogona kuomeswa muRNA stabilization tube (eg RNAstable®) uye inotumirwa mukupisa kwekamuri.

Kuyerera Kwebasa Rebasa

Muenzaniso QC

Kuedza kugadzira

sampuli yekuendesa

Muenzaniso wekutumira

Pilot experiment

RNA kubviswa

Kugadzirira Raibhurari

Kuvakwa kweraibhurari

Sequencing

Sequencing

Data analysis

Data analysis

Mushure mekutengesa Services

Mushure mekutengesa masevhisi


  • Zvakapfuura:
  • Zvinotevera:

  • Bioinformatics

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    RNA kutaura muchidimbu

     图片41

    Differentially Akaratidza majini

    图片42

     

     

    ceRNA kuongorora

     图片43Yakasiyana inoratidzwa miRNAs uye ine hukama RNAs

    图片44 

     Ongorora kufambira mberi kwekutsvagisa kunofambiswa neBMKGene 'yose transcriptome sequencing masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.

     

    Dai, Y. et al.(2022) 'Comprehensive expression profiles of mRNAs, lncRNAs uye miRNAs muKashin-Beck chirwere chakaonekwa neRNA-sequencing', Molecular Omics, 18 (2), pp. 154-166.doi: 10.1039/D1MO00370D.

    Liu, N. nan et al.(2022) 'Full urefu transcriptomes wongororo yekutonhora-kuramba kweApis cerana muChangbai Mountain panguva yechando.', Gene, 830, pp. 146503–146503.doi: 10.1016/J.GENE.2022.146503.

    Wang, XJ nevamwe.(2022) 'Multi-Omics Kubatanidzwa-Yakavakirwa Kutungamira Kwekukwikwidza Endogenous RNA Regulation Networks muDiki Cell Lung Cancer: Molecular Characteristics uye Drug Candidates', Frontiers muOncology, 12, p.904865. doi: 10.3389/FONC.2022.904865/BIBTEX.

    Xu, P. et al.(2022) 'Kuongorora kwakabatanidzwa kwelncRNA/circRNA-miRNA-mRNA kutaura kweprofiles kunoratidza ruzivo rutsva mune zvingangoita nzira mukupindura midzi-knot nematodes munzungu', BMC Genomics, 23 (1), mapeji 1-12.doi: 10.1186/S12864-022-08470-3/FIGURES/7.

    Yan, Z. et al.(2022) 'Whole-transcriptome RNA sequencing inosimbisa nzira dzemamorekuru dzine chekuita nekuchengetedzwa kwemhando yepostharvest mu broccoli nered LED irradiation', Postharvest Biology and Technology, 188, p.111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.

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