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Alaabta

Bakteeriyada Genome oo Dhamaystiran

Teknolojiyadda Biomarker waxay bixisaa adeeg isku xigxig ah oo lagu dhisayo genome-ka bakteeriyada oo dhammaystiran oo aan lahayn farqi eber ah.Shaqada ugu muhiimsan ee bakteeriyadu waxay dhamaystiraysaa dhismaha genome-ga waxaa ka mid ah isku xigxiga jiilka saddexaad, isu-ururinta, sharraxaadda shaqeynta iyo falanqaynta bioinformatic-ka sare ee buuxinaysa yoolalka cilmi-baarista gaarka ah.Bakteeriyada genome-ka ee genome-ka ayaa awood u siinaya muujinta hababka aasaasiga ah ee ka hooseeya hababka bayoolojiga, taas oo sidoo kale siin karta tixraac qiimo leh cilmi-baarisyada genomic ee noocyada sare ee eukaryotic.

Platform:Nanopore PromethION P48 + Illumina NovaSeq Platform

Talaabada PacBio II


Faahfaahinta Adeegga

Natiijooyinka Demo

Faa'iidooyinka Adeegga

● Xeelado isku xigxig oo kala duwan oo loo heli karo ujeedooyin cilmi baaris oo kala duwan

● Bakteeriyadu waxay dhammaystiran tahay genome oo leh 0 Gap dammaanad ah.

● Khibrad sare u leh bakteeriyada genome-ga oo la isku keenay in ka badan 10,000 oo genom oo dhamaystiran.

● Kooxda taageerada farsamada iibka ka dib ee xirfadlayaasha ah oo buuxinaya baahiyo cilmi-baaris oo gaar ah.

Tilmaamaha Adeegga

Isku xigxigaXeeladda

Maktabadda

Tayada la damaanad qaaday

Waqtiga soo-jeedinta la qiyaasay

Nanopore 100X + Illumina 50X

Nanopore 20K

PE150

0 Farqiga

30 Maalmood

PacBio HiFi 30X

PacBio 10K

Falanqaynta bioinformatics

● Xakamaynta tayada xogta ceeriin

● Kulanka genome

● Falanqaynta qaybaha genome

● Sharaxaada shaqada hidda-wadaha

● Falanqaynta genomic isbarbardhigga

2

Tusaalaha Shuruudaha iyo Bixinta

Tusaalaha Shuruudaha:

WaayoSoosaarida DNA:

Tusaalaha Nooca

Qadarka

Xoog-saarid

daahirnimo

Soosaarida DNA

2 μg

> 20 ng/μl

OD260/280= 1.6-2.5

Shaybaarada unugyada:

Tusaalaha nooca daawaynta muunad ee lagu taliyay Tusaalaha kaydinta iyo rarista
Bakteeriyada Bakteeriyada ku fiiri mikroskoob ka dibna ku ururi bakteeriyada wejigeeda jibbaarada

U wareeji dhaqanka bakteeriyada (oo ka kooban qiyaastii 3-4.5e9 unug) una wareeji 1.5 ama 2 ml eppendorf.(Barafka ku hay)

Centrifuge tuubada 1 daqiiqo at 14000 g si aad u ururiso bakteeriyada oo aad si taxadar leh uga saartid dusha sare

Xidh tuubada oo ku qabooji bakteeriyada ku jirta nitrogen dareeraha ah ugu yaraan 30 daqiiqo.Ku kaydi tuubada qaboojiyaha -80 ℃.

Ku qabooji shaybaarada ku jira nitrogen dareeraha ah 3-4 saacadood kuna kaydi nitrogen dareeraha ah ama -80 digrii ilaa boos sii hayn muddo dheer ah.Tusaalaha rarida baraf qallalan ayaa loo baahan yahay.

Socodka Shaqada Adeegga

muunad keenid

Tusaalaha keenista

Diyaarinta Maktabadda

Dhismaha maktabadda

Isku xigxiga

Isku xigxiga

Falanqaynta xogta

Falanqaynta xogta

Adeegyada iibka kadib

Adeegyada iibka kadib


  • Hore:
  • Xiga:

  • 1.Circos of bakteeriyada genome

    3

    2.Coding saadaasha hiddaha

    4

    3.Falanqaynta genomics isbarbardhigga: geedka phylogenetic

    5

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