Isbarbardhigga Genomics
Faa'iidooyinka Adeegga
● Xidhmada falanqaynta oo dhamaystiran, oo ka kooban siddeedda falanqayn ee inta badan loo baahan yahay
● Isku halaynta sare ee falanqaynta oo leh tafsiir faahfaahsan oo si fudud loo fahmi karo natiijooyinka
● Tirooyinka diyaarsan ee si wanaagsan loo qaabeeyey
● Kooxda bioinformatics ee xirfad sare leh waxay buuxisaa shuruudaha falanqaynta shakhsi ahaaneed ee kala duwan
● Wakhti gaaban oo soo-jeedin ah oo leh saxnaansho sare marka loo eego falanqaynta
● Khibrad badan oo leh in ka badan 90 kiis oo guul leh oo leh saameyn la daabacay oo la daabacay oo ka badan 900
Tilmaamaha Adeegga
| Waqtiga soo-jeedinta la qiyaasay | Tirada noocyada | Falanqeeya |
| 30 maalmood oo shaqo ah | 6 - 12 | Kooxda hidaha ee qoyska Hindaha qoyska balaadhinta iyo foosha Dhisidda geedka phylogenetic Qiyaasta wakhtiga kala duwanaanshaha (is-habaynta fosil ayaa loo baahan yahay) Waqtiga gelinta LTR (Dhirta) Ku duubida genome-ga oo dhan ( Dhirta) Cadaadis doorasho Falanqaynta synteny |
Falanqaynta bioinformatics
● Qoyska hidaha
● Filogenetics
● Wakhtiga kala duwanaanshaha
● Cadaadis doorasho
● Falanqaynta synteny
Tusaalaha Shuruudaha iyo Bixinta
Tusaalaha Shuruudaha:
Nudaha ama DNA-da ee isku xigxiga genome-ga iyo isu-ururinta
Wixii Nudaha
| Noocyada | Nudaha | Sahan | PacBio CCS |
| Xoolaha | Nudaha indhaha | 0.5 ~ 1g | ≥ 3.5g |
| Nudaha murqaha | |||
| ≥ 5.0g | |||
| ≥ 5.0ml | |||
| Dhiiga naasleyda | |||
| ≥ 0.5ml | |||
| Dhiiga digaaga/kalluunka | |||
| Dhirta | Caleen Cusub | 1 ~ 2g | ≥ 5.0g |
| Dufan/jirrid | 1 ~ 2g | ≥ 10.0g | |
| Xidid/Shiil | 1 ~ 2g | ≥ 20.0g | |
| Unugyada | Unug dhaqan | - | ≥ 1 x 108 |
Xogta
Faylasha isku xigxiga genome(.fasta) iyo faylalka annotation (.gff3) ee noocyada aad isugu dhow
Socodka Shaqada Adeegga
Naqshadaynta tijaabada
Tusaalaha keenista
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
*Natiijooyinka demo ee halkan lagu muujiyey waxay dhamaantood ka yimaaddeen genome-yada lagu daabacay Tignoolajiyada Biomarker
1.LTR gelin qiyaasta wakhtiga: Shaxanku waxa uu muujiyay qaybinta laba-geesoodka ah ee gaarka ah ee LTR-RTs wakhtiyada gelinta ee Weining galley genome, marka la barbar dhigo noocyada kale.Kii ugu sarreeyay wuxuu soo baxay qiyaastii 0.5 milyan sano ka hor.
Li Guang et al.,Hiddaha Dabeecada, 2021
2. Falanqaynta Phylogeny iyo hidda-wadaha qoyska ee chayote (Sechium edule): Marka la falanqeeyo chayote iyo 13ka nooc ee kale ee la xidhiidha qoyska hidda-wadaha, Chayote waxa la ogaaday in ay aad ugu dhawdahay in ay xidhiidh la leedahay geedka abeesada (Trichosanthes anguina).Chayote oo ka soo jeeda geedka abeesada qiyaastii 27-45 Mya iyo genome nuqul ka mid ah (WGD) ayaa lagu arkay chayote gudaha 25± 4 Mya, taas oo ah dhacdadii saddexaad ee WGD ee cucuibitaceae.
Fu A iyo al.,Cilmi-baarista Dhirta, 2021
3.Synteny falanqaynta: Qaar ka mid ah hiddo-wadaha la xidhiidha phytohormones ee horumarinta miraha ayaa laga helay chayote, geedka abeesada iyo squash.Xidhiidhka u dhexeeya chayote iyo squash ayaa waxyar ka sarreeya kan u dhexeeya chayote iyo geedka abeesada.
Fu A iyo al.,Cilmi-baarista Dhirta, 2021
Falanqaynta qoyska 4.Gene: kobcinta KEGG ee balaadhinta hidda-wadaha qoyska iyo foosha ee G.thurberi iyo G.davidsonii genomes waxay muujisay in biosynthesis steroid iyo brassinosteroid biosynthesis la xidhiidha hiddo-wadaha la ballaariyay.
Yang Z iyo al.,BMC Biology, 2021
5. Falanqaynta genome-ga oo dhan: 4DTV iyo falanqaynta qaybinta Ks ayaa muujisay dhacdo nuqul ka mid ah genome-ga oo dhan.Meesha ugu sarreysa ee noocyada intraspecies ayaa muujiyay dhacdooyin nuqul ka mid ah.Meelaha ugu sarreeya ee noocyada kala duwan ayaa muujiyay dhacdooyin gaar ah.Falanqaynta ayaa muujisay in marka la barbar dhigo saddexda nooc ee kale ee xidhiidhka dhow la leh, O. europaea waxay soo martay hiddo-wadareed baaxad weyn dhawaanahan.
Rao G iyo al.,Cilmi-baarista Dhirta, 2021
Kiiska BMK
Rose bilaa prickle: aragtida genomic ee ku xidhan la qabsiga qoyaanka
La daabacay: Dib u eegista Sayniska Qaranka, 2021
Istaraatiijiyada isku xigxiga:
'Baasye'sQodax la'aan(R.Wichurainangenome:
Qiyaastii93 X PacBio + qiyaastii.90 X Nanopore + 267 X Illumina
Natiijooyinka muhiimka ah
1.Tayada sare ee R.wichuraiana genome waxaa lagu dhisay iyadoo la isticmaalayo farsamooyinka isku xigxiga ee dheer-akhrinta, taas oo keentay kulan ah 530.07 Mb (Qiyaastii genome size ahaa ku dhawaad 525.9 Mb by socodka cytometry iyo 525.5 by genome sahanka； Heterozygosity ahaa agagaarka 1.03%).BUSCO waxay ku qiyaastay buundada 93.9%.Marka la barbar dhigo "Blush Old" (haploOB), tayada iyo dhammaystirka genome-kan waxaa lagu xaqiijiyay saxnaanta hal-saldhigga iyo tusmada kulanka LTR (LAI=20.03).R.wichuraiana genome waxa uu ka kooban yahay 32,674 hiddo-sidaha koodka borotiinka.
2.Multi-omics wadajir falanqeyn, oo ka kooban genomics isbarbardhigga, transcriptomics, QTL falanqaynta dadweynaha hidde, ayaa shaaca ka qaaday sifada muhiimka ah ee u dhexeeya R. wichuraiana iyo Rosa chinensis.Sidoo kale, kala duwanaanshiyaha muujinta hiddo-wadaha laxiriira ee QTL waxay u badan tahay in lala xiriiriyo qaabaynta asliga ah.
Genomics isbarbardhigga ah ee u dhexeeya Basye;s Thornless iyo Rosa chinensis oo ay ku jiraan falanqaynta synteny, kooxda hidde-sideyaasha qoyska, balaadhinta iyo falanqaynta foosha, ayaa shaaca ka qaaday tiro badan oo kala duwanaansho ah, kuwaas oo la xidhiidha sifooyinka muhiimka ah ee ubaxyada.Balaadhinta gaarka ah ee NAC iyo FAR1/FRS qoyska hiddaha waxay aad ugu dhowdahay inay la xiriirto iska caabinta barta madow.
Falanqaynta isbarbardhigga genomics ee u dhaxaysa BT iyo genomes haploOB.
Zhong, M., iyo al."Rose without prickle: aragtida genomic ee ku xiran la qabsiga qoyaanka"Dib u eegista Sayniska Qaranka, 2021;, nwab092.
