Alaabta

Tignoolajiyada isku xigxiga ee Illumina, oo ku salaysan Taxane by Synthesis (SBS), waa hal-abuur caalami ah oo la aqbalay NGS, oo mas'uul ka ah soo saarista in ka badan 90% xogta isku xigxiga ee adduunka.Mabda'a SBS waxa ay ku lug leedahay sawirida shucaaca leh ee lagu calaamadiyay joojinta dib loo rogi karo marka dNTP kasta lagu daro, ka dibna la kala qaado si loogu ogolaado ku darida saldhiga xiga.Iyada oo dhammaan afarta dNTP-yada ku xidhan ee dib loo rogi karo ay ku jiraan meerto kasta oo taxane ah, tartanka dabiiciga ahi waxa uu yareeyaa eexda ku darista.Tiknoolajiyadan wax-ku-oolka ah waxay taageertaa maktabadaha hal-akhriska ah iyo kuwa-dhamaadka-dhamaadka ah, iyada oo u adeegaysa noocyo badan oo codsiyo genomic ah.Awoodaha wax soo saarka sare ee Illumina iyo sida saxda ah waxay u dhigayaan inay tahay tiir udub dhexaadka cilmi baarista genomics, taasoo awood siinaysa saynisyahanada inay daaha ka rogaan qalafsanaanta genome-yada oo leh tafatir iyo hufnaan aan isbarbar dhigin.

DNBSEQ, oo ay soo saartay BGI, waa tignoolajiyada cusub ee NGS oo ku guulaysatay inay hoos u dhigto kharashyada isku xigxiga iyo kordhinta wax soo saarka.Diyaarinta maktabadaha DNBSEQ waxay ku lug leedahay kala-goynta DNA-da, diyaarinta ssDNA iyo wareegga wareegga wareegga si loo helo DNA nanoballs (DNB).Kuwan ayaa markaa lagu raraa dusha adag oo ka dibna la isku xigxigay isku-darka Baadhista-Anchor Synthesis (cPAS).

Adeegeena isku xigxiga maktabadda ee horay loo sameeyay waxa uu u sahlayaa macaamiisha diyaarinta isku xigxiga maktabadahooda ilo kala duwan (mRNA, genom dhan, amplicon, iyo kuwo kale).Ka dib, maktabadahan waxaa loo rari karaa xarumahayada isku xigxiga ee ilaalinta tayada iyo isku xigxiga ee goobaha Illumina ama BGI.