Hidde-sidayaasha Horumarka
Faa'iidooyinka Adeegga
Takagi iyo kuwa kale,Joornaalka dhirta, 2013
●Falanqaynta dhammaystiran ee bioinformatics:awood u siineysa qiyaasta kala duwanaanshaha hidde-sidaha, kaas oo ka tarjumaya awoodda isbeddelka ee noocyada, iyo muujinta xiriirka phylogenetic ee la isku halleyn karo ee u dhexeeya noocyada leh saameyn yar oo ku saabsan horumarka isku-dhafan iyo horumarka isbarbar socda
●Falanqayn la habeeyey oo ikhtiyaari ah: sida qiyaasta waqtiga iyo xawaaraha kala duwanaanshaha iyadoo lagu saleynayo kala duwanaanshaha heerka nucleotide iyo amino acids.
●Khibrad ballaaran iyo diiwaanno daabacaadBMKGene waxay soo ururisay waayo-aragnimo ballaaran oo ku saabsan mashaariicda hidde-sidaha ee dadka iyo isbeddelka muddo ka badan 15 sano, iyadoo daboolaysa kumanaan nooc, iwm. waxayna gacan ka geysatay in ka badan 1000 mashruuc oo heer sare ah oo lagu daabacay Isgaarsiinta Dabeecadda, Dhirta Molecular, Joornaalka Bayotechnoolajiyadda Dhirta, iwm.
● Kooxda bioinformatics oo xirfad sare leh iyo wareegga falanqaynta gaaban: Iyadoo khibrad weyn u leh falanqaynta genomics-ka horumarsan, kooxda BMKGene waxay bixisaa falanqayn dhammaystiran oo leh waqti degdeg ah oo wax laga beddelayo.
● Taageerada Iibka Kadib:Ballanqaadkeennu wuxuu ka baxsan yahay dhammaystirka mashruuca iyadoo la adeegsanayo muddo 3 bilood ah oo adeeg ah ka dib iibka. Inta lagu jiro waqtigan, waxaan bixinnaa dabagalka mashruuca, caawinta xallinta dhibaatooyinka, iyo fadhiyada su'aalaha iyo jawaabaha si wax looga qabto su'aalaha la xiriira natiijooyinka.
Qeexitaannada Adeegga iyo shuruudaha
| Nooca taxanaha | Tirada dadka lagu taliyay | Istaraatiijiyadda taxanaha | Shuruudaha Nukliyeerka |
| Kala-soocidda Hidde-sidayaasha oo Dhan | ≥ 30 qof, oo leh ≥ 10 qof oo ka kala socda koox kasta
| 10x | Xoog-saarid: ≥ 1 ng/µL Wadarta tirada≥ 30ng Hoos u dhac ama wasakheyn xaddidan ama aan jirin |
| Jajab la xoojiyay oo gaar ah (SLAF) | Qoto dheer ee calaamadda: 10x Tirada calaamadaha: <400 Mb: WGS ayaa lagu talinayaa <1Gb: 100K oo calaamado ah 1Gb >2Gb: 300K oo calaamado ah Ugu badnaan 500k oo tags ah | Xoog-saarid ≥ 5 ng/µL Wadarta guud ≥ 80 ng Nanodrop OD260/280=1.6-2.5 Jel Agarose ah: burbur ama wasakh aan lahayn ama xaddidan
|
Socodka Shaqada Adeegga
Naqshadeynta tijaabada
Bixinta muunad
Dhismaha maktabadda
Taxanaha
Falanqaynta Xogta
Adeegyada iibka kadib
Adeeggu wuxuu ka kooban yahay falanqaynta qaab-dhismeedka dadka (geedka phylogenetic-ga, PCA, jaantuska kala-soocidda dadka), kala duwanaanshaha dadka, iyo xulashada dadka (isku-xidhka aan sinnayn, xulashada xulashada goobaha faa'iidada leh). Adeeggu wuxuu sidoo kale ku jiri karaa falanqayn la habeeyey (tusaale ahaan waqtiga kala-soocidda, socodka hidda-wadaha).
*Natiijooyinka demo ee halkan lagu muujiyay dhammaantood waa hidde-sideyaal lagu daabacay BMKGENE
1. Falanqaynta Horumarku waxay ka kooban tahay dhismaha geedka phylogenetic-ga, qaab-dhismeedka dadweynaha iyo PCA oo ku salaysan kala duwanaanshaha hidde-sidaha.
Geedka phylogenetic wuxuu matalaa xiriirro taxonomic iyo isbeddel ah oo ka dhexeeya noocyada leh awoowe caadi ah.
PCA waxay higsaneysaa inay sawirto isku soo dhawaansho u dhaxaysa dadka hoose.
Qaab-dhismeedka dadweynaha wuxuu muujinayaa joogitaanka dad-hoosaadyo kala duwan oo hidde ahaan ah marka loo eego soo noqnoqoshada allele.
Chen, iwm.,PNAS, 2020
2. Xariiq xulasho leh
Xaraashka xulashada ah waxaa loola jeedaa habka lagu doorto goob faa'iido leh oo la kordhiyo soo noqnoqoshada goobaha dhexdhexaadka ah ee isku xiran iyo kuwa goobaha aan isku xirnayn la dhimo, taasoo keenta hoos u dhac ku yimaada gobolka.
Ogaanshaha hidde-sideyaasha ee gobollada xulashada leh waxaa lagu farsameeyaa iyadoo la xisaabinayo tusmada hidde-sidaha dadweynaha (π, Fst, Tajima's D) ee dhammaan SNP-yada ku jira daaqad simbiriirixan (100 Kb) tallaabo gaar ah (10 Kb).
Kala duwanaanshaha Nukliyeerka (π)
Tajima's D
Tusmada hagaajinta (Fst)
Wu, iwm.,Warshad Molecular ah, 2018
3. Socodka Hidde-sidaha
Wu, iwm.,Warshad Molecular ah, 2018
4. Taariikhda dadka
Zhang, iwm.,Deegaanka iyo Horumarka Dabeecadda, 2021
5. Waqtiga kala-guurka
Zhang, iwm.,Deegaanka iyo Horumarka Dabeecadda, 2021
Sahamin horumarka ay fududeeyeen adeegyada hidde-sidaha ee isbeddelka BMKGene iyada oo loo marayo ururin la soo xulay oo daabacado ah:
Hassanyar, AK et al. (2023) 'Soo saarista Calaamadaha Molecular-ka SNP iyo Hidde-sidayaasha Musharrixiinta ee la xiriira iska caabbinta fayraska Sacbrood ee Apis cerana cerana Larvae iyadoo la adeegsanayo Dib-u-eegista Hidde-sidayaasha oo Dhan',Joornaalka caalamiga ah ee cilmiga molecular-ka, 24 (7). doi: 10.3390/IJMS24076238.
Chai, J. et al. (2022) 'Soo helitaanka salamander weyn oo Shiinees ah oo duurjoogta ah oo hidde ahaan saafi ah waxay abuurtaa fursado cusub oo ilaalin ah',Cilmi-baarista Xoolaha, 2022, Vol. 43, Cadadka 3aad, Bogagga: 469-480, 43(3), boggaga 469–480. doi: 10.24272/J.ISSN.2095-8137.2022.101.
Han, M. et al. (2022) 'Qaab-dhismeedka Fiisiyoolajiyadeed iyo Taariikhda Horumarka Dadweynaha ee Dadka Asaliga ah ee Elymus sibiricus L. oo ku taal Buurta Qinghai-Tibetan',Xuduudaha Sayniska Dhirta, 13, boggaga 882601. doi: 10.3389/FPLS.2022.882601/BIBTEX.
Wang, J. et al. (2022) 'Fikradaha hidde-sideyaasha ee ku saabsan horumarka longan ee ka imanaya isu-imaatinka hidde-sideyaasha heerka koromosoomka iyo hidde-sideyaasha dadweynaha ee isku-darka longan',Cilmi-baarista Beeraha, 9. doi: 10.1093/HR/UHAC021.
