TGuide Smart Blood/Cutubka RNA Kit
Lambarka buugaagta / baakadaha
| Bisad maya | ID | Tirada diyaarinta |
| 4995043 (taariko) | DP661-E | 96 |
| 4993551 (kartridge) | DP661-DE | 48 |
Hagaha xulashada Reagent ee TGuide S16
| Nuclei Acid | Tusaalaha Nooca | Magaca Alaabta | Cabbirka baakadka (diyaarinta) Kartuuji/ saxan | Bisadda maya. Kartuun/saanad |
| DNA | Nudaha Xoolaha | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Dhirta & Abuurka | Qalabka DNA-da ee Dhirta Birlabka leh ee TGuide | 48 | 4993548 |
| DNA | Ciid/ Saxaro | TGuide Smart Ciid / Qalabka DNA ee saxarada | 48 | 4993549 |
| DNA | Alaabooyinka jel & PCR | Qalabka Sifaynta DNA ee casriga ah ee TGuide | 48 | 4993550 |
| DNA | Dhiig dhan | TGuide Smart Dhiigga Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Unug/Swab/Meelaha Qalalan, iwm | TGuide Smart Universal Qalabka DNA | 48/96 | 4993704/4995040 |
| RNA | Dhiiga / Unugyada | TGuide Smart Blood/Cutubka RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Dhirta & Abuurka | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Dheecaannada Unugyada Xorta ah (Serum, iwm) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
hordhaca alaabta
Ka nadiifi wax-soo-saarka sare, nadiif-sare, wadarta guud ee RNA-ga tayada sare leh ee dhiigga/unugga/unugga.
Ma jiraan wax sun ah sida phenol/chloroform oo ku jira
RNA-da la helay waxay leedahay daahirnimo sare waxaana si toos ah loogu isticmaali karaa ogaanshaha chips, isku xigxiga-sare iyo tijaabooyin kale.
Astaamaha
Aad u fudud in la isticmaalo
Ma jirto shaqo tuubo oo dheeraad ah.
Mugga elution waxaa loo hagaajin karaa sida loo baahdo.
Rakibaadda iyo hawlgalku waxay u baahan yihiin tababar yar. Barnaamijyada horay loo rakibay, ka soo saar kartoonada, dooro borotokoolka oo socodsii tijaabadaada. Barnaamijyadu waa diyaar in la isticmaalo, sidoo kalena la beddeli karo.
Dib-u-dejiyayaal hore loo raray iyo agabka la tuuri karo
Maaraynta kiimikada waa la tirtiraa ilaa xadka ugu badan si loo xaqiijiyo badbaadada hawlgalka.
Natiijada Demo
Agilent 2100 ayaa loo isticmaalay ogaanshaha daacadnimada.
Tusaalaha isha: CD-1 jiirarka
Xaaladda tusaale: dhiig cusub oo dhan
Cabbirka Tusaalaha: 200 μl
Natiijada tijaabada: wadarta guud ee RNA laga soo saaray dhiiga TGuide S16 oo ku qalabaysan reagent 4993551 waxay leedahay wax soo saar wanaagsan iyo nadiif sare, waxayna dabooli kartaa baahiyaha dhinaca hoose.
isku xigxiga-sare.
Tusaalaha isha: CD-1 jiirarka
Mugga soo dejinta: 1 μl. Beerka ayaa la qasi jiray 5 jeer oo la raray.
Feejignaanta Gel Agarose: 1%. Electrophoresis 20 min at 6 V / cm;
M:TIANGEN calaamade III 1-4: wadnaha 5-8:
beerka 9-12: sambabada 13-16: kelyaha
Labada shay ee ugu horreeya ee unug kasta waxaa soo saaray xirmada soo saarista ku salaysan spin-column, iyo labadii ugu dambeeyay waxaa si toos ah u soo saaray TGuide S16.
Natiijada tijaabada: wadarta unugyada xayawaanka ee RNA si toos ah u soo saaray TGuide S16 oo ku qalabaysan reagent 4993551 waxay leedahay waxsoosaar sare iyo daahirnimo, taas oo u dhiganta RNA ay soo saartay nidaamka soo saarista lafdhabarta. Nadiifintu asal ahaan waa isku mid, oo leh OD260 / OD280 agagaarka 2.0 ~ 2.1 iyo OD260 / OD230> 2.0. Gebogebadii, TGuide S16 waxaa loo isticmaali karaa in laga soo saaro wadarta RNA ee unugyada xayawaanka beddelka xalka soo saarista ku salaysan lafdhabarta.
