BMKCloud gal

Alaabta

Taxanaha Metatranscriptome

Ka faa'iidaysiga tignoolajiyada isku xigxiga ee Illumina, adeegga isku xigxiga ee metatranscriptome ee BMKGENE ayaa daaha ka qaadaya muujinta firfircoonida hidda-wadaha ee noocyo kala duwan oo microbes ah, oo u dhexeeya eukaryotes ilaa prokaryotes iyo fayrasyada, gudaha deegaannada dabiiciga ah sida ciidda, biyaha, badda, saxarada, iyo xiidmaha. Adeegeena dhamaystiran waxa uu awooda saarayaa cilmi-baarayaasha in ay dhexgalaan bulshooyinka microbial-ka adag ee muuqaalada muujinta hidda-wadaha. Marka laga soo tago falanqaynta taxonomic, adeegeena isku xigxiga ee metatranscriptome waxa uu fududeeyaa sahaminta kobcinta shaqaynta, iftiiminta hiddo-wadaha si kala duwan loo muujiyay iyo doorkooda. Soo hel fikrado badan oo bayooloji ah markaad dhex socoto muuqaalada kakan ee muujinta hidde-sidaha, kala duwanaanta taxonomiga, iyo firfircoonida shaqaynaysa gudaha meelahan kala duwan ee deegaanka.

Nala Soo Xidhiidh

Faahfaahinta Adeegga

Bioinformatics

Natiijooyinka Demo

Daabacaada sifada

Tilmaamaha Adeegga

● Dhimasho rRNA ah oo ay ku xigto diyaarinta maktabadda mRNA jihada.

● Taxanaha Illumina NovaSeq.

Faa'iidooyinka Adeegga

●Baro Isbeddelada Bulshooyinka Microbial-ka ee Isku-dhafan:Tani waxay ku dhacdaa heerka qoraalka waxayna sahamisaa hiddo-wadaha cusub ee suurtagalka ah.

●Sharaxaada isdhexgalka Bulshada Microbial ee martida loo yahay ama deegaanka.

●Falanqaynta Bioinformatic ComprehensiveTani waxay ku siinaysaa aragtiyo ku saabsan cashuurta bulshada iyo halabuurka shaqaynaysa, iyo sidoo kale falanqaynta muujinta hidde-sidaha kala duwan.

●Tilmaanta Hidde-wadaagaha oo Balaaran:Isticmaalka xogta shaqada hidda-wadaha ee casriga ah ee macluumaadka muujinta hidda-wadaha ee bulshooyinka microbial-ka ah.

●Taageerada Iibka Kadib:Ballanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib. Inta lagu jiro wakhtigan, waxaan bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto su'aalo kasta oo la xidhiidha natiijooyinka.

Tilmaamaha Adeegga

Madal isku xigxiga	Istaraatiijiyada Taxanaha	Xogta lagu taliyay	Xakamaynta tayada xogta
Illumina NovaSeq	PE150	12Gb	Q30≥85%

Shuruudaha Tusaalaha

Xooga saarida (ng/µL)	Tirada guud (µg)	Mugga (µL)	OD260/280	OD260/230	RIN
≥50	≥1.0	≥20	1.8-2.0	1.0-2.5	≥6.5

Socodka Shaqada Adeegga

Tusaalaha keenista

Dhismaha maktabadda

Isku xigxiga

Falanqaynta xogta

Adeegyada iibka kadib

Kii hore: De novo Fungal Genome Assembly

Xiga: Taxanaha RNA Prokaryotic

Waxa ku jira falanqaynta soo socota:

● Ilaalinta tayada xogta

● Qoraalka qoraalka

● Taxonomic Taxonomic iyo Badan

● Sharaxaad Waxqabad iyo Badin

● Tirakoobka Muujinta iyo Falanqaynta Kala Duwanaanshaha

Qaybinta Taxonomic ee muunad kasta:

Falanqaynta kala duwanaanta beta: UPGMA

Tilmaanta shaqaynaysa - GO badnaan

Baaxadda cashuuraha kala duwan - LEFSE

Sahami horumarka ay fududaysay BMKGene adeegyadeeda isku xigxiga ee transcriptomics-ka iyada oo loo marayo ururinta daabacaado la soo xulay.

Lu, Z. iyo al. (2023) 'Dulqaadka aysiidhka ee bakteeriyada-lactate-isticmaalka nidaamka Bacteroidal waxay gacan ka geysataa ka hortagga ruminal acidosis ee riyaha oo la qabsaday cunto diirad sare leh',Nafaqada Xoolaha, 14, bogga 130-140. doi: 10.1016/J.ANINU.2023.05.006.

Hees, Z. iyo al. (2017) 'Foojinta microbiota asaasiga ah ee shaqaynaysa halsano-dhaqmeed adag oo lagu sameeyay amplicon-ka sare iyo isku xigxiga metatranscriptomics',Xuduudaha Microbiology, 8 (July). doi: 10.3389/FMICB.2017.01294/FULL.

Wang, W. iyo al. (2022) 'Novel Mycoviruses ayaa laga helay Sahan Metatranscriptomics ee Fungus Alternaria Phytopathogenic',Fayrasyada, 14(11), b. 2552. doi: 10.3390/V14112552/S1.

Wei, J. iyo al. (2022) 'Falanqaynta metatranscriptome isbarbar socda ayaa daaha ka qaadaysa hoos u dhaca dheef-shiid kiimikaad sare ee dhirta ee kuwa lamid ah iyo calaacalaha mindhicirka',Molecular Ecology, 31 (15), bogga 3999-4016. doi: 10.1111/MEC.16557.