Taxanaha RNA-yar-Illumina
Astaamaha
● Diyaarinta cabbirka xulashada maktabadda.
● Falanqaynta bioinformatic waxay ku salaysan tahay saadaasha miRNA iyo bartilmaameedyada.
Faa'iidooyinka Adeegga
●Khibrad Balaadhan: Waxaan farsameynay in ka badan 300 oo muunado, oo ka kooban noocyo kala duwan oo muunado ah. Mashruuc kasta waxa aanu keenaynaa hodantinimada khibradda.
●Xakamaynta tayada adagWaxaan hirgelinnaa qodobbada kontoroolka asaasiga ah ee dhammaan heerarka, laga bilaabo diyaarinta muunad ilaa diyaarinta maktabadda, isku xigxiga iyo bioinformatics. Korjoogteyna taxadar leh waxay hubisaa gaarsiinta natiijooyin tayo sare leh oo joogto ah.
●Falanqaynta bioinformatics oo dhammaystiran:Waxay awood u siinaysaa aqoonsiga miRNA-yada cusub ee la yaqaan iyo kuwa cusub, aqoonsiga bartilmaameedyada miRNA-yada, iyo sharraxaadda shaqaynaysa ee u dhiganta iyo kobcinta xog ururin badan (KEGG, GO).
●Taageerada Iibka Kadib: Waxaan fahamsanahay sida ay muhiim u tahay in la jooggo, taasina waa sababta ballan-qaadkeennu uu dhaafsiisan yahay dhammaystirka mashruuca oo leh 3 bilood ka dib adeegga iibka. Inta lagu jiro wakhtigan, waxaanu bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto wixii su'aalo ah ee la xidhiidha natiijooyinka.
Tusaalaha Shuruudaha iyo Bixinta
| Maktabadda | Platform | Xogta lagu taliyay | Xogta QC |
| Cabbirka la doortay | Illumina SE50 | 10M-20M ayaa akhriya | Q30≥85% |
Tusaalaha Shuruudaha:
Nucleotides:
| Conc.(ng/μl) | Qadarka (μg) | daahirnimo | Daacadnimo |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Xaddidan ama aan lahayn borotiin ama wasakh DNA oo lagu muujiyey jel. | RIN≥6.0; 5.0≥28S/18S≥1.0; xaddidan ama aan lahayn sare u qaadis aasaasi ah |
● Dhirta:
Xididka, stem ama Petal: 450 mg
Caleen ama Iniin: 300 mg
Miraha: 1.2 g
● Xoolaha:
Wadnaha ama mindhicirka: 450 mg
Viscera ama Maskaxda: 240 mg
Murqaha: 600 mg
Lafaha, timaha ama maqaarka: 1.5g
● Arthropods:
Cayayaanka: 9g
Crustacea: 450 mg
● Dhiig dhan: 2 tuubbo
Unugyada: 106 unugyo
● Serum iyo Plasma:6 ml
Tusaalaha keenista lagu taliyay
Weel: 2 ml oo tuubo sentrifuge ah (Faylada qasacadaysan laguma talinayo)
Tusaalaha calaamadaynta: Kooxda+ku celcelinta tusaale A1, A2, A3; B1, B2, B3.
Raritaanka:
1. Barafka qallalan: Muunadodu waxay u baahan yihiin in lagu xidho bac oo lagu aaso baraf qallalan.
2. Tuubooyinka RNAstable: Muunada RNA waxaa lagu qallajin karaa tuubada xasilinta RNA (tusaale RNAstable®) waxaana lagu rari karaa heerkulka qolka.
Socodka Shaqada Adeegga
Naqshadaynta tijaabada
Tusaalaha keenista
soo saarida RNA
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
Bioinformatics
● Xakamaynta tayada xogta ceeriin
● Kala soocida RNA yar
● MiRNA muujinta iyo falanqaynta muujinta kala duwanaanta
● Hidde-sidaha bartilmaameedka ee miRNA iyo sharraxaad hidde-sidaha bartilmaameedka miRNA oo si kala duwan loo muujiyay
● Faahfaahinta hiddo-wadaha bartilmaameedka ah iyo kobcinta shaqada hidda-wadaha miRNA oo si kala duwan loo muujiyay
Aqoonsiga miRNA: qaab dhismeedka iyo qoto dheer
Tibaaxaha kala duwan ee miRNA - ururinta kala sareynta
Sharaxaadda shaqaynaysa ee bartilmaameedka miRNA-yada kala duwan ee la soo bandhigay
Sahami horumarka cilmi-baarista ee ay fududeeyeen adeegyada isku xigxiga ee BMKGene sRNA iyada oo loo marayo ururinta daabacaado la soo xulay.
Chen, H. iyo al. (2023) 'Infekshannada fayrasku waxay joojiyaan biosynthesis saponin iyo photosynthesis ee Panax notoginseng',Physiology Plant iyo Biochemistry, 203, p. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. iyo al. (2023) 'Warshada FYVE domain-ka ka kooban borotiin FREE1 waxay la xidhiidhaa qaybaha microprocessor si loo cadaadiyo miRNA biogenesis',Warbixinta EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. iyo al. (2023) 'MicroRNA Ame-Bantam-3p' waxay xakameysaa horumarinta Pupal Larval iyadoo la beegsanayo Xurmada Kobaca Epidermal-ka ee u eg Domains 8 Gene (megf8) ee Honeybee, Apis mellifera',Joornaalka Caalamiga ah ee Cilmiga Molecular, 24(6), b. 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. iyo al. (2018) 'Falanqaynta Isku-dhafka ah ee MiRNA iyo Hidde-yaasha Ku Xidhan Tayada Hilibka Waxay Muujinaysaa in Gga-MiR-140-5p Ay Saamayso Dhigista Dufanka Muruqa ee Digaagga',Physiology Cellular iyo Biochemistry, 46(6), bogga 2421–2433. doi: 10.1159/000489649.
