Kala-soocidda RNA-yar-Illumina
Astaamaha
● Diyaarinta maktabadda xulashada cabbirka.
● Falanqaynta bayoolojiga ee ku salaysan saadaasha miRNA iyo bartilmaameedyada.
Faa'iidooyinka Adeegga
●Khibrad Ballaaran: Waxaan ka shaqeynay in ka badan 300 oo muunado, oo ku kala duwan noocyada muunado kala duwan. Waxaan khibrad badan u keenaynaa mashruuc kasta.
●Xakamaynta Tayada Adag: Waxaan hirgelinnaa qodobbada xakamaynta asaasiga ah dhammaan marxaladaha, laga bilaabo diyaarinta muunadda ilaa diyaarinta maktabadda, taxanaha iyo bioinformatics. Kormeerkayaga taxaddarka leh wuxuu hubinayaa bixinta natiijooyin tayo sare leh oo joogto ah.
●Falanqaynta dhammaystiran ee bioinformatics:Waxay awood u siineysaa aqoonsiga miRNA-yada la yaqaan iyo kuwa cusub labadaba, aqoonsashada bartilmaameedyada miRNA-yada, iyo sharraxaadda shaqada ee u dhiganta iyo kobcinta iyadoo la adeegsanayo xog ururin badan (KEGG, GO).
●Taageerada Iibka Kadib: Waxaan fahamsanahay sida ay muhiim u tahay in la joogo, waana sababta ballanqaadkeennu uu uga dheeraado dhammaystirka mashruuca iyadoo la adeegsanayo muddo 3 bilood ah oo adeegga iibka kadib ah. Inta lagu jiro waqtigan, waxaan bixinnaa dabagalka mashruuca, caawinta xallinta dhibaatooyinka, iyo fadhiyada su'aalaha iyo jawaabaha si wax looga qabto su'aalaha la xiriira natiijooyinka.
Shuruudaha Muunadda iyo Bixinta
| Maktabad | Madal | Xogta lagu taliyay | Xogta QC |
| Cabbirka la doortay | Illumina SE50 | Akhrinta 10M-20M | Q30≥85% |
Shuruudaha Muunadda:
Nukliyeeydh:
| Conc. (ng/μl) | Caddadka (μg) | Daahirnimo | Daacadnimo |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Wasakhowga borotiinka ama DNA-da oo xaddidan ama aan lahayn ayaa lagu muujiyay jelka. | RIN≥6.0; 5.0≥28S/18S≥1.0; kor u kac aasaasi ah oo xaddidan ama aan lahayn |
● Dhirta:
Xidid, Jirrid ama Ubax: 450 mg
Caleen ama Iniinta: 300 mg
Miro: 1.2 g
● Xayawaanka:
Wadnaha ama Mindhicirka: 450 mg
Xididdada ama Maskaxda: 240 mg
Muruq: 600 mg
Lafo, Timo ama Maqaarka: 1.5g
● Arthropods:
Cayayaanka: 9g
Crustacea: 450 mg
● Dhiig dhan: 2 tuubo
● Unugyada: 106 unugyada
● Serum iyo Balaasma:6 mL
Bixinta Muunadda ee lagu taliyay
Weel: 2 ml tuubo centrifuge ah (Daasadda laguma talinayo)
Calaamadaynta tusaalaha ah: Koox+nuqul tusaale ahaan A1, A2, A3; B1, B2, B3.
Rarista:
1. Baraf Qallalan: Muunadaha waa in lagu xidhaa bacaha oo lagu aaso baraf qalalan.
2. Tuubooyinka RNA-ga ee la hagaajin karo: Muunadaha RNA-ga waxaa lagu qallajin karaa tuubada xasilinta RNA (tusaale ahaan RNAstable®) waxaana loo rari karaa heerkulka qolka.
Socodka Shaqada Adeegga
Naqshadeynta tijaabada
Bixinta muunad
Soo saarista RNA
Dhismaha maktabadda
Taxanaha
Falanqaynta Xogta
Adeegyada iibka kadib
Bayoinformatics
● Xakamaynta tayada xogta ceeriin
● Kala soocidda RNA-da yar
● muujinta miRNA iyo falanqaynta muujinta kala duwan
● Hiddo-wadaha bartilmaameedka miRNA iyo sharraxaadda hiddo-wadaha bartilmaameedka miRNA ee si kala duwan loo muujiyey
● Sharaxaadda hidda-wadaha bartilmaameedka ah iyo kobcinta shaqada hidda-wadaha miRNA ee si kala duwan loo muujiyey
Aqoonsiga miRNA: qaab-dhismeedka iyo qoto dheeraanta
Muujinta kala duwanaanshaha miRNA - kooxaynta kala sarreynta
Faahfaahinta shaqaynta ee bartilmaameedka miRNA-yada si kala duwan loo muujiyey
Sahamin horumarka cilmi-baarista ee ay fududeeyeen adeegyada taxanaha sRNA ee BMKGene iyada oo loo marayo ururin la soo xulay oo daabacado ah.
Chen, H. et al. (2023) 'Caabuqyada fayrasku waxay joojiyaan biosynthesis-ka saponin iyo sawir-qaadista Panax notoginseng',Fiisiyoolajiyadda Dhirta iyo Bayoolajiyadda, 203, boggaga 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) 'Warshadda FYVE domain-ka ka kooban borotiinka FREE1 waxay la xiriirtaa qaybaha microprocessor-ka si loo xakameeyo bayogenesis-ka miRNA',Warbixinnada EMBO, 24 (1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'MicroRNA Ame-Bantam-3p waxay xakameysaa horumarka dirxiga ee carruurta iyadoo la bartilmaameedsanayo 8-da hiddo-wadaha (megf8) ee shinnida malabka, Apis mellifera',Joornaalka Caalamiga ah ee Sayniska Molekulelka, 24(6), bogga 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al. (2018) 'Falanqaynta Isku-dhafan ee MiRNA iyo Hidde-sidayaasha La Xiriira Tayada Hilibka ayaa Muujinaya in Gga-MiR-140-5p ay Saameyso Kala-goynta Dufanka Muruqyada Dhexdooda ee Digaagga',Fiisiyoolajiyada Unugga iyo Bayoolajiyada, 46(6), bogga 2421–2433. doi: 10.1159/000489649.
