Isku xigxiga RNA-yar-Illumina
Astaamaha
● Xulashada cabbirka RNA ka hor diyaarinta maktabadda
● Falanqaynta bioinformatics waxay ku salaysan tahay saadaasha miRNA iyo bartilmaameedyadooda
Faa'iidooyinka Adeegga
●Falanqaynta bioinformatics oo dhammaystiran:suurtagelinta aqoonsiga miRNA-yada cusub ee la yaqaan iyo kuwa cusub, aqoonsiga bartilmaameedyada miRNA-yada iyo sharraxaadda shaqada ee u dhiganta iyo kobcinta xog ururin badan (KEGG, GO)
●Xakamaynta tayada adagWaxaan hirgelineynaa qodobbada kontoroolka asaasiga ah ee dhammaan heerarka, laga bilaabo muunad iyo diyaarinta maktabadda ilaa isku xigxiga iyo bioinformatics.Kormeerkan taxaddar leh wuxuu xaqiijinayaa gaarsiinta natiijooyin tayo sare leh oo joogto ah.
●Taageerada Iibka KadibBallanqaadkayaga waxa uu dhaafsiisan yahay dhamaystirka mashruuca oo leh 3 bilood oo adeega iibka kadib.Inta lagu jiro wakhtigan, waxaan bixinaa dabagalka mashruuca, caawinta cilad-raadinta, iyo fadhiyada Q&A si wax looga qabto su'aalo kasta oo la xidhiidha natiijooyinka.
●Khibrad Balaadhan: iyada oo si guul leh loo xirayo mashaariic badan oo sRNA ah oo daboolaya in ka badan 100 nooc oo cilmi-baarisyo kala duwan ah, kooxdeenu waxay u keentaa khibrad badan mashruuc kasta.
Tusaalaha Shuruudaha iyo Bixinta
| Maktabadda | Platform | Xogta lagu taliyay | Xogta QC |
| Cabbirka la doortay | Illumina SE50 | 10M-20M ayaa akhriya | Q30≥85% |
Tusaalaha Shuruudaha:
Nucleotides:
| Conc.(ng/μl) | Qadarka (μg) | daahirnimo | Daacadnimo |
| ≥ 80 | ≥ 0.5 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Xaddidan ama aan lahayn borotiin ama wasakh DNA oo lagu muujiyey jel. | RIN≥6.5; 5.0≥28S/18S≥1.0; xaddidan ama aan lahayn sare u qaadis aasaasi ah |
● Dhirta:
Xididka, stem ama Petal: 450 mg
Caleen ama abuur: 300 mg
Miraha: 1.2 g
● Xoolaha:
Wadnaha ama mindhicirka: 450 mg
Viscera ama Maskaxda: 240 mg
Murqaha: 600 mg
Lafaha, timaha ama maqaarka: 1.5g
● Arthropods:
Cayayaanka: 9g
Crustacea: 450 mg
● Dhiig dhan: 2 tuubbo
Unugyada: 106 unugyo
● Serum iyo Plasma:6 ml
Tusaalaha keenista lagu taliyay
Konteenar:
2 ml oo tuubo sentrifuge ah (Faylada qasacadaysan laguma talinayo)
Tusaalaha calaamadaynta: Kooxda+ku celcelinta tusaale A1, A2, A3;B1, B2, B3......
Raritaanka:
1.Baraf qallalan: Tijaabooyinku waxay u baahan yihiin in lagu xidho bacaha oo lagu aaso baraf qallalan.
Tubooyinka RNAstable: Muunada RNA waxaa lagu qallajin karaa tuubada xasilinta RNA (tusaale RNAstable®) waxaana lagu rari karaa heerkulka qolka.
Socodka Shaqada Adeegga
Naqshadaynta tijaabada
Tusaalaha keenista
soo saarida RNA
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
Bioinformatics
Aqoonsiga miRNA: qaab dhismeedka iyo qoto dheer
Tibaaxaha kala duwan ee miRNA - ururinta kala sareynta
Sharaxaadda shaqaynaysa ee bartilmaameedka miRNA-yada kala duwan ee la soo bandhigay
Sahami horumarka cilmi-baarista ee ay fududeeyeen adeegyada isku xigxiga ee BMKGene sRNA iyada oo loo marayo ururinta daabacaado la soo xulay.
Chen, H. iyo al.(2023) 'Infekshannada fayrasku waxay joojiyaan biosynthesis saponin iyo photosynthesis ee Panax notoginseng', Fiisioloji Dhirta iyo Biochemistry, 203, p.108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. iyo al.(2023) Geedka FYVE domain-ka ku jira borotiinka FREE1 wuxuu la xidhiidhaa qaybaha microprocessor si loo cadaadiyo miRNA biogenesis', warbixinnada EMBO, 24(1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. iyo al.(2023) 'MicroRNA Ame-Bantam-3p' waxay xakameysaa Horumarinta Pupal Larval iyadoo la beegsanayo Xurmada Kobaca Epidermal-ka ee Kala Duwan ee Domains 8 Gene (megf8) ee Honeybee, Apis mellifera', Joornaalka Caalamiga ah ee Sayniska Molecular, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. iyo al.(2018) 'Falanqaynta Isku-dhafka ah ee MiRNA iyo Hidde-yaasha Ku Xidhan Tayada Hilibka Waxay Muujinaysaa in Gga-MiR-140-5p Saamayso Dhigista Dufanka Intramuscular ee Digaagga', Physiology Cellular iyo Biochemistry, 46(6), pp. 2421-2433.doi: 10.1159/000489649.
