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Special Price for Next Generation Sequencing Cost - Fungal Genome – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
üRaw data quality control
üGenome assembly
üGenome component analysis
üGene function annotation
üComparative genomic analysis

Sample Requirements and Delivery
Sample Requirements:
For DNA extracts:
|
Sample Type
|
Amount
|
Concentration
|
Purity
|
|
DNA extracts
|
> 3 μg
|
> 20 ng/μl
|
OD260/280= 1.6-2.5
|
For tissue samples:
| Sample type |
Recommended sample treatment |
Sample storage and shipment |
| Unicellular Fungus |
Observe yeast under microscope and collect them in their exponential phase
Transfer the culture (containing approx. 3-4.5e9 cells) into a 1.5 or 2 ml eppendorf. (Keep on ice)
Centrifuge the tube for 1 min at 14000 g to collect bacteria and remove the supernatant carefully
Seal the tube and freeze the bacteria in liquid nitrogen for at least 30 min. Store the tube in -80 ℃ fridge. |
Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required. |
| Macro Fungus |
Tissue in actively growing phase is recommended.
Rinse the tissue with endotoxin-free water, then 70% ethonal.
Save the sample in cryo-tubes. |
Service Work Flow

Sample delivery

Library construction

Sequencing

Data analysis

After-sale services
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