Lihlahisoa

Genome e Felletseng Libaktheria

Baktheria e Feletseng Genome Featured Image

Biomarker Technologies e fana ka ts'ebeletso ea tatellano mabapi le ho aha genome e felletseng ea libaktheria tse nang le lekhalo le zero.Ts'ebetso ea mantlha ea libaktheria kaho e felletseng ea liphatsa tsa lefutso e kenyelletsa tatellano ea moloko oa boraro, kopano, tlhaloso e sebetsang le tlhahlobo e tsoetseng pele ea bioinformatics e phethahatsang lipheo tse khethehileng tsa lipatlisiso.Profil e felletseng ea libaktheria tsa genome e fana ka matla a ho senola mekhoa ea mantlha ea ts'ebetso ea bona ea tlhaho, e ka fanang ka leseli la bohlokoa bakeng sa lipatlisiso tsa genomic mefuteng e phahameng ea eukaryotic.

Sethala:Nanopore PromethION P48 + Illumina NovaSeq Platform

PacBio Sequel II

Iteanye le rona

Lintlha tsa Tšebeletso

Demo Results

Melemo ea Tšebeletso

● Mekhoa e mengata ea tatellano e teng bakeng sa lipakane tse fapaneng tsa lipatlisiso

● Baktheria e feletseng ea genome e nang le 0 Gap e tiisitsoeng.

● E na le phihlelo e ngata ea ho kopanya liphatsa tsa lefutso tsa baktheria 'me liphatsa tsa lefutso tse fetang 10,000 li kopantsoe.

● Sehlopha sa tšehetso sa botekgeniki ka mor'a thekiso se phethahatsang litlhoko tse khethehileng tsa lipatlisiso.

Litlhaloso tsa Tšebeletso

TatelanoLeano

Laebrari

Boleng bo tiisitsoe

Khakanyo ea nako ea ho fetoha

Nanopore 100X + Illumina 50X

Nanopore 20K

PE150

0 Lekhalo

Matsatsi a 30

PacBio HiFi 30X

PacBio 10K

Litlhahlobo tsa Bioinformatics

● Taolo ea boleng ba data e tala

● Kopano ea Genome

● Tlhahlobo ea likarolo tsa genome

● Tlhaloso ea tšebetso ea liphatsa tsa lefutso

● Ho bapisa genomic analysis

Litlhoko tsa Mohlala le Phano

Litlhoko tsa Mohlala:

Bakeng saLintlha tsa DNA:

Mofuta oa Mohlala	Chelete	Ho tsepamisa maikutlo	Bohloeki
Lintlha tsa DNA	＞ 2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Bakeng sa mehlala ea li-tissue:

Mofuta oa mohlala

Phekolo ea mohlala e khothalelitsoeng

Mohlala oa polokelo le thomello

Baktheria

Sheba libaktheria ka tlas'a microscope 'me u bokelle libaktheria sebakeng sa tsona sa exponential

Fetisetsa setso sa baktheria (e nang le lisele tse ka bang 3-4.5e9) ho eppendorf ea 1.5 kapa 2 ml.(Keep on ice)

Centrifuge tube bakeng sa 1 min ho 14000 g ho bokella libaktheria le ho tlosa supernatant ka hloko.

Koala tube 'me u hatsele libaktheria ka naetrojene e metsi bonyane metsotso e 30.Boloka tube ka sehatsetsing sa -80 ℃.

Emisa lisampole ka har'a naetrojene e metsi bakeng sa lihora tse 3-4 'me u li boloke ka har'a metsi a naetrojene kapa -80 degree ho boloka nako e telele.Ho hlokahala ho romelloa ka sekepe se nang le leqhoa le omileng.