Libaktheria le Fungus Whole Genome Re-Sequencing
Merero ea tatellano ea liphatsa tsa lefutso tsa baktheria le fungal kaofela ke ea bohlokoa bakeng sa ho ntšetsa pele liphatsa tsa lefutso tsa likokoana-hloko ka ho etsa hore ho phetheloe le ho bapisa liphatsa tsa lefutso tsa microbial. Sena se nolofatsa boenjiniere ba ho belisoa, ho ntlafatsa mekhoa ea indasteri, le ho hlahloba mekhoa ea bobeli ea metabolism. Ho feta moo, tatellano e ncha ea fungal le baktheria e bohlokoa bakeng sa ho utloisisa phetoho ea tikoloho, ho ntlafatsa mefuta, le ho senola matla a ho iphetola ha liphatsa tsa lefutso, ka litlamorao tse pharaletseng ho tsa bongaka, temo le mahlale a tikoloho.
Melemo ea Tšebeletso
●Tlhahlobo ea Bioinformatiki e kenyelletsa ho letsa ho fapaneng:Ho fana ka lintlha tse sebetsang ho liphatsa tsa lefutso tse latellanang hape.
● Tsebo e Pharalletseng: Ka likete tsa merero ea tatellano e ncha ea likokoana-hloko e etsoang selemo le selemo, re tlisa lilemo tse fetang leshome tsa phihlelo, sehlopha sa batho ba nang le tsebo e phahameng ea ho hlahloba, litaba tse feletseng, le tšehetso e babatsehang ea ka mor'a thekiso.
●Tšehetso ea Ka mor'a Thekiso:Boitlamo ba rona bo fetela ka nģ'ane ho ho phethoa ha morero ka nako ea tšebeletso ea likhoeli tse 3 ka mor'a thekiso. Nakong ena, re fana ka tatellano ea projeke, thuso ea ho rarolla mathata, le linako tsa Q&A ho araba lipotso life kapa life tse amanang le sephetho.
Litlhaloso tsa Tšebeletso
| Sethala sa tatellano | Leano la Tatelano | Lintlha li khothalelitsoe | Taolo ea boleng |
| Illumina NovaSeq | PE150 | Botebo ba 100x | Q30≥85% |
Litlhoko tsa Mohlala
| Khokahano (ng/µL) | Kakaretso (ng) | Bolumo (µL) |
| ≥1 | ≥60 | ≥20 |
Baktheria: ≥1x107 lisele
Li-fungus tsa Unicellular: ≥5x106-1x107 lisele
Macro Fung: ≥4g
Phallo ea Mosebetsi oa Ts'ebeletso
Mohlala oa thomello
Kaho ea laebrari
Tatelano
Tlhahlobo ea lintlha
Litšebeletso tsa ka mor'a thekiso
E kenyelletsa Tlhahlobo e Latelang:
- Ho Sequencing Taolo ea Boleng ba Boitsebiso
- Ho tsamaisana le Reference Genome
- Ho letsa ho fapaneng: SNP le InDel
- Phapang Annotation
Mehala e fapaneng: Mefuta ea SNP
Ho letsa ho fapaneng: Kabo ea bolelele ba InDel
Lekola tsoelo-pele e tsamaisitsoeng ke BMKGene's microbial genome re-sequencing services ka pokello e hlophisitsoeng ea likhatiso.
Jia, Y. et al. (2023) 'Ho kopanya Transcriptome le Whole Genome Re-Sequencing to Screen Disease Resistance Genes for Wheat Dwarf Bunt',Koranta ea machaba ea saense ea limolek'hule, 24(24). doi: 10.3390/IJMS242417356.
Jiang, M. et al. (2023) 'Ampicillin-controlled glucose metabolism e laola phetoho ho tloha mamellong ho ea ho hanyetsa libaktheria',Tsoelo-pele ea Saense, 9(10). doi: 10.1126/SCIADV.ADE8582/SUPPL_FILE/SCIADV.ADE8582_SM.PDF.
Yang, M. et al. (2022) 'Aliidiomarina halalkaliphila sp. nov., baktheria ea haloalkaliphilic e thōko letšeng la soda ho Inner Mongolia Autonomous Region, Chaena', Int. J. Syst. Evol.Microbiol, 72, leq. 5263. doi: 10.1099/ijsem.0.005263.
Zhu, Z., Wu, R. le Wang, G.-H. (2024) 'Genome sequence of Staphylococcus nepalensis ZZ-2023a, e arohaneng le Nasonia vitripennis',Liphatlalatso tsa Mohloli oa Microbiology. doi: 10.1128/MRA.00802-23.
