Ho Latela Botjha Dijini Tsohle tsa Baktheria le tsa Fungus
Merero ea ho hlophisa bocha liphatsa tsa lefutso tsa baktheria le fungus ke ea bohlokoa bakeng sa ho ntšetsa pele liphatsa tsa lefutso tsa likokoana-hloko ka ho nolofalletsa ho phethoa le ho bapisoa ha liphatsa tsa lefutso tsa likokoana-hloko. Sena se nolofalletsa boenjiniere ba ho belisa, ntlafatso ea lits'ebetso tsa indasteri, le ho hlahloba litsela tsa bobeli tsa metabolism. Ho feta moo, ho hlophisa bocha liphatsa tsa lefutso le baktheria ho bohlokoa bakeng sa ho utloisisa ho ikamahanya le tikoloho, ho ntlafatsa mefuta, le ho senola matla a ho iphetola ha liphatsa tsa lefutso, ka litlamorao tse pharaletseng bongaka, temo le saense ea tikoloho.
Melemo ea Tšebeletso
●Tlhahlobo ea Bioinformatic e kenyelletsa ho bitsa ho fapaneng:Ho fana ka temohisiso e sebetsang mabapi le li-genome tse hlophisitsoeng bocha.
● Bokgoni bo Boholo: Ka merero e likete-kete ea ho hlophisa bocha likokoana-hloko e etsoang selemo le selemo, re tlisa boiphihlelo ba lilemo tse fetang leshome, sehlopha sa tlhahlobo se nang le boiphihlelo bo phahameng, litaba tse felletseng, le tšehetso e ntle ea kamora thekiso.
●Tšehetso ea Kamora Thekiso:Boitlamo ba rona bo fetela ka nģ'ane ho ho phethoa ha projeke ka nako ea tšebeletso ea likhoeli tse 3 kamora thekiso. Nakong ena, re fana ka tlhahlobo ea projeke, thuso ea ho rarolla mathata, le lipuisano tsa lipotso le likarabo ho araba lipotso life kapa life tse amanang le liphetho.
Litlhaloso tsa Tšebeletso
| Sethala sa tatellano | Leano la Tatellano | Lintlha tse khothaletsoang | Taolo ea boleng |
| Illumina NovaSeq | PE150 | Botebo ba 100x | Q30≥85% |
Litlhoko tsa Sampole
| Ho tsepamisa maikutlo (ng/µL) | Kakaretso ea chelete (ng) | Bophahamo ba modumo (µL) |
| ≥1 | ≥60 | ≥20 |
Libaktheria: ≥1x107 lisele
Li-fungus tse nang le sele e le 'ngoe: ≥5x106-1x107 lisele
Li-fungus tse kholo: ≥4g
Phallo ea Mosebetsi oa Tšebeletso
Phano ea mohlala
Kaho ea laeborari
Tatellano
Tlhahlobo ea lintlha
Litšebeletso tsa kamora thekiso
E kenyelletsa Tlhahlobo e Latelang:
- Taolo ea Boleng ba Lintlha e Latellanang
- Ho lumellana le Genome ea Reference
- Ho bitsa ho fapaneng: SNP le InDel
- Tlhaloso e fapaneng
Mefuta e fapaneng ea ho bitsa: Mefuta ea SNP
Mohala o fapaneng: Kabo ea bolelele ba InDel
Hlahloba tsoelo-pele e tsamaisitsoeng ke lits'ebeletso tsa BMKGene tsa ho hlophisa bocha liphatsa tsa lefutso tsa likokoana-hloko ka pokello ea lingoliloeng tse hlophisitsoeng.
Jia, Y. et al. (2023) 'Ho Kopanya Transcriptome le Genome Eohle ho Latela Diphatsa tsa lefutso tsa ho Hanela Mafu a Sekoaelo bakeng sa Koro e Nyenyane',Koranta ea machaba ea saense ea limolek'hule, 24(24). doi: 10.3390/IJMS242417356.
Jiang, M. et al. (2023) 'Metabolism ea tsoekere e laoloang ke Ampicillin e laola phetoho ho tloha mamellong ho ea ho hanyetsa libaktheria',Tsoelo-pele ea Saense, 9(10). doi: 10.1126/SCIADV.ADE8582/SUPPL_FILE/SCIADV.ADE8582_SM.PDF.
Yang, M. et al. (2022) 'Aliidiomarina halalkaliphila sp. nov., baktheria ea haloalkaliphilic e arohileng letšeng la soda sebakeng se ikemetseng sa Inner Mongolia, Chaena', Int. J. Syst. Evol.Microbiol, 72, leq. 5263. doi: 10.1099/ijsem.0.005263.
Zhu, Z., Wu, R. le Wang, G.-H. (2024) 'Tatellano ea liphatsa tsa lefutso tsa Staphylococcus nepalensis ZZ-2023a, e arotsoeng ho tsoa ho Nasonia vitipennis',Litsebiso tsa Lisebelisoa tsa Microbiology.doi: 10.1128/MRA.00802-23.
