BMKMANU S1000 Spatial Transcriptome
BMKMANU S1000 Spatial Transcriptome Technical Scheme
Melemo ea BMKMANU S1000
1) Qeto ea li-sub-cellular: Sebaka se seng le se seng se nkiloeng se na le > Li-Barcoded Spots tse limilione tse 2 tse bophara ba 2.5 µm le sebaka sa 5 µm lipakeng tsa litsi tsa matheba, se nolofalletsang tlhahlobo ea transcriptome ea sebaka ka qeto ea sub-cellular (5 µm).
2) Tlhahlobo ea tharollo ea maemo a mangata: Tlhahlobo e feto-fetohang ea maemo a mangata ho tloha ho 100 μm ho ea ho 5 μm ho rarolla likarolo tse fapaneng tsa lisele ka tharollo e nepahetseng.
3) Phatlalatso e felletseng ea li-transcriptome: Lingoloa tse hapiloeng ho slide eohle ea lithane li ka hlahlojoa, ntle le thibelo ea palo ea liphatsa tsa lefutso le sebaka se shebiloeng.
Litlhaloso tsa Tšebeletso
| Laebrari | Leano la tatellano | Data Output e khothalelitsoe |
| S1000 cDNA library | BMKMANU S1000-Illumina PE150 | 60Gb / mohlala |
Litlhoko tsa Mohlala
| Mohlala | Nomoro | Boholo | RNA Boleng |
| OCT e kentsoeng ka lithane tse thibelang | 2-3 liboloko/ sampole | Hoo e ka bang.6.8x6.8x6.8 limilimithara3 | RIN≥7 |
Bakeng sa lintlha tse ling mabapi le tataiso ea boitokisetso ea mohlala le ts'ebetso ea ts'ebeletso, ka kopo ikutloe u lokolohile ho bua le aBMKGENE setsebi
Phallo ea Mosebetsi oa Ts'ebeletso
Moqapi oa liteko
Mohlala oa thomello
Teko pele
Sebaka sa barcoding
Kaho ea laebrari
Tatelano
Tlhahlobo ea lintlha
Litšebeletso tsa ka mor'a thekiso
Lintlha tse hlahisitsoeng ke BMKMANU S1000 li hlahlojoa ho sebelisoa software "BSTMatrix", e entsoeng ka boikemelo ke BMKGENE, ho kenyelletsa:
1) Ho hlahisa liphatsa tsa lefutso tsa matrix
2) Ho sebetsana le litšoantšo tsa HE
3) E lumellana le software ea motho oa boraro bakeng sa tlhahlobo
4) Online "BSTViewer" e thusa ho fumana liphetho tsa pono ka liqeto tse fapaneng.
1.Spot clustering
2.Kabo ea sebaka
Note: Qetoboemo=13 (100 µm, le letšehali); 7 (50 µm, ka ho le letona)
3.Marker expression abundance clustering heatmap
4.Inter-sample data analysis
