TGuide Smart Mali/Cell/Tissue RNA Kit
Nomoro ea lethathamo / sephutheloana
| Katse. che | ID | No. ea preps |
| 4995043(plate) | DP661-E | 96 |
| 4993551(khatriji) | DP661-DE | 48 |
Tataiso ea khetho ea reagent bakeng sa TGuide S16
| Asiti ea Nuclei | Mofuta oa Mohlala | Lebitso la Sehlahiswa | Boholo ba ho paka (preps) Cartridge/ poleiti | Nomoro ea katse. Cartridge/ poleiti |
| DNA | Lithapo tsa Liphoofolo | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Semela & Peō | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Mobu/Sentle | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| DNA | Lihlahisoa tsa Gel & PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Madi Kaofela | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Dry Spots, joalo-joalo | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Mali/Seleng/Mahlapa | TGuide Smart Mali/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Semela & Peō | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Mekelikeli ea Mahala ea Lisele (Serum, joalo-joalo) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Kenyelletso ea sehlahisoa
Hloekisa RNA ea boleng bo holimo, bohloeki bo phahameng, boleng ba boleng bo holimo ho tsoa maling / liseleng / liseleng.
Ha ho li-reagents tse chefo tse kang phenol/chloroform tse nang le tsona
RNA e fumanoeng e na le bohloeki bo phahameng 'me e ka sebelisoa ka ho toba bakeng sa ho lemoha chip, tatellano ea maemo a holimo le liteko tse ling.
Likaroloana
Ho bonolo haholo ho e sebelisa
Ha ho na mosebetsi o eketsehileng oa ho penta.
Bolumo ea elution e ka fetoloa kamoo ho hlokahalang.
Ho kenya le ho sebetsa ho hloka koetliso e fokolang. Ka mananeo a kentsoeng pele, bula cartridge, khetha protocol 'me u tsamaise liteko tsa hau. Mananeo a se a loketse ho sebelisoa, hape a ka etsoa ka mokhoa o ikhethileng.
Li-reagents tse kentsoeng esale pele le lisebelisoa tse ka sebelisoang tse tsamaisanang.
Ho sebetsana le lik'hemik'hale ho felisoa ka tekanyo e kholo ka ho fetisisa ho netefatsa ts'ireletso ea ts'ebetso.
Sephetho sa Demo
Agilent 2100 e ne e sebelisetsoa ho lemoha botšepehi.
Mohloli oa mohloli: litoeba tsa CD-1
Boemo ba mohlala: mali a foreshe
Boholo ba mohlala: 200 μl
Sephetho sa teko: kakaretso ea RNA e ntšitsoeng maling ke TGuide S16 e nang le reagent 4993551 e na le chai e ntle le bohloeki bo phahameng, 'me e ka finyella litlhoko tsa ho theoha tlaase.
tatellano ya maemo a hodimo.
Mohloli oa mohloli: litoeba tsa CD-1
Bophahamo ba ho kenya: 1 μl. Sebete se ile sa hlapolloa ka makhetlo a 5 'me sa kenngoa.
Khatiso ea gel ea Agarose: 1%. Electrophoresis bakeng sa metsotso e 20 ho 6 V / cm;
M:TIANGEN Lesupa III 1-4: pelo 5-8:
sebete 9-12: matšoafo 13-16: liphio
Mehlala ea pele e 'meli ea lesela le leng le le leng e ile ea ntšoa ke "spin-column based extraction kit", 'me tse peli tsa ho qetela li ile tsa ntšoa ka bo eona ke TGuide S16.
Sephetho sa liteko: kakaretso ea RNA e ntšitsoeng ka bo eona ke TGuide S16 e nang le reagent 4993551 e na le chai e ngata le bohloeki, e lekanang le RNA e ntšitsoeng ke protocol ea "spin-column based extraction". Bohloeki bo tšoana hantle, ka OD260/OD280 ho pota 2.0 ~ 2.1 le OD260/OD230> 2.0. Qetellong, TGuide S16 e ka sebelisoa ho ntša RNA eohle ho tloha liseleng tsa liphoofolo ho nkela sebaka sa "spin-column based extraction solution".
