Lihlahisoa

Genome ea fungal

Biomarker Technologies e fana ka tlhahlobo ea genome, genome e ntle le genome e felletseng ea fungal ho latela sepheo se itseng sa lipatlisiso.Tatelano ea liphatsa tsa lefutso, kopano le tlhaloso e sebetsang e ka finyelloa ka ho kopanya tatellano ea moloko o latelang + Tatelano ea moloko oa boraro ho finyella kopano ea boemo bo phahameng ba genome.Theknoloji ea Hi-C e ka boela ea sebelisoa ho tsamaisa kopano ea genome boemong ba chromosome.

Sethala:PacBio Sequel II

Nanopore PromethION P48

Illumina NovaSeq Platform

Iteanye le rona

Lintlha tsa Tšebeletso

Demo Results

Melemo ea Tšebeletso

● Mekhoa e mengata ea tatellano e teng bakeng sa lipakane tse fapaneng tsa lipatlisiso

● E na le phihlelo e ngata ea ho kopanya liphatsa tsa lefutso tsa li-fungus tse fetang 10,000 tsa pene-complete genome tse kopantsoeng.

● Sehlopha sa tšehetso sa botekgeniki ka mor'a thekiso se phethahatsang litlhoko tse khethehileng tsa lipatlisiso.

Litlhaloso tsa Tšebeletso

Tshebeletso	Leano la Tatelano	Boleng bo tiisitsoe	Khakanyo ea nako ea ho fetoha
'Mapa o motle oa fungal	Illumina 50X+Nanopore 100X	Contig N50≥2 Mb	35 matsatsi a ho sebetsa
'Mapa o motle oa fungal	PacBio HiFi 30X	Contig N50≥2 Mb	35 matsatsi a ho sebetsa
'Mapa o felletseng oa pene ea fungal	Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Chromosome anchoring ratio>90%	45 matsatsi a ho sebetsa

Litlhahlobo tsa Bioinformatics

● Taolo ea boleng ba data e tala

● Kopano ea Genome

● Tlhahlobo ea likarolo tsa genome

● Tlhaloso ea tšebetso ea liphatsa tsa lefutso

● Ho bapisa genomic analysis

Litlhoko tsa Mohlala le Phano

Litlhoko tsa Mohlala:

Bakeng saLintlha tsa DNA:

Mofuta oa Mohlala	Chelete	Ho tsepamisa maikutlo	Bohloeki
Lintlha tsa DNA	＞ 1.2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Bakeng sa mehlala ea li-tissue:

Mofuta oa mohlala

Phekolo ea mohlala e khothalelitsoeng

Mohlala oa polokelo le thomello

Li-fungus tsa Unicellular

Sheba tomoso tlas'a microscope 'me u li bokelle karolong ea tsona ea exponential

Fetisetsa setso (e nang le lisele tse ka bang 3-4.5e9) ho 1.5 kapa 2 ml eppendorf.(Keep on ice)

Centrifuge tube bakeng sa 1 min ho 14000 g ho bokella libaktheria le ho tlosa supernatant ka hloko.

Koala tube 'me u hatsele libaktheria ka har'a metsi a naetrojene bonyane lihora tse 1-3.Boloka tube ka sehatsetsing sa -80 ℃.

Emisa lisampole ka har'a naetrojene e metsi bakeng sa lihora tse 3-4 'me u li boloke ka har'a metsi a naetrojene kapa -80 degree ho boloka nako e telele.Ho hlokahala ho romelloa ka sekepe se nang le leqhoa le omileng.

Macro Fungus

Ho kgothaletswa hore ho be le mesifa e ntseng e hola ka mafolofolo.

Hlatsoa lisele ka metsi a se nang endotoxin, ebe 70% ethonal.

Boloka sampole ho cryo-tubes.