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Semela/Phoofolo De novo Genome Sequencing
Ho fihlela joaletatellano e bolela kaho ea mofuta oohle oa lefutso ho sebelisoa theknoloji ea tatelano, mohlala, PacBio, Nanopore, NGS, joalo-joalo, ntle le genome ea referense.Ntlafatso e tsotehang ea bolelele ba ho bala ba theknoloji ea tatellano ea moloko oa boraro e tlisitse menyetla e mecha ea ho bokella liphatsa tsa lefutso tse rarahaneng, tse kang tse nang le heterozygosity e phahameng, karo-karolelano e phahameng ea libaka tse pheta-phetoang, li-polyploid, joalo-joalo. ho rarolla lintlha tse ipheta-phetang, libaka tse nang le litaba tsa GC tse sa tloaelehang le libaka tse ling tse rarahaneng haholo.
Sethala: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq6000
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Hi-C e thehiloeng ho Genome Assembly
Hi-C ke mokhoa o etselitsoeng ho ts'oara tlhophiso ea chromosome ka ho kopanya litšebelisano tse thehiloeng ho proximity le tatellano ea maemo a holimo.Ho lumeloa hore matla a litšebelisano tsena a amana hampe le sebaka sa 'mele ho li-chromosome.Ka hona, lintlha tsa Hi-C li ka tataisa ho kopanya, ho hlophisa le ho tsamaisa tatellano e kopaneng ho genome e hlophisitsoeng le ho tiisa tseo palo e itseng ea li-chromosome.Theknoloji ena e matlafatsa kopano ea liphatsa tsa lefutso tsa boemo ba chromosome ha ho se na 'mapa oa lefutso o thehiloeng ho baahi.Genome e 'ngoe le e' ngoe e hloka Hi-C.
Sethala: Illumina NovaSeq6000 / DNBSEQ
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Liphatsa tsa lefutso tsa ho iphetola ha lintho
Evolutionary genetics ke ts'ebeletso e hlophisitsoeng ea tatelano e etselitsoeng ho fana ka tlhaloso e felletseng mabapi le tlhaiso-leseling ea ho iphetola ha lintho ea lisebelisoa tse fanoeng tse ipapisitseng le mefuta ea liphatsa tsa lefutso, ho kenyeletsoa SNPs, InDels, SVs le CNVs.E fana ka litlhahlobo tsohle tsa motheo tse hlokahalang bakeng sa ho hlalosa liphetoho tsa ho iphetola ha lintho le likarolo tsa lefutso tsa baahi, joalo ka sebopeho sa baahi, mefuta-futa ea liphatsa tsa lefutso, likamano tsa phylogeny, joalo-joalo. E boetse e na le lithuto tsa phallo ea liphatsa tsa lefutso, tse matlafatsang khakanyo ea boholo bo sebetsang ba baahi, nako ea ho fapana.
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Papiso ea Genomics
Papiso ea genomics ha e le hantle e bolela ho bapisa tatellano e felletseng ea liphatsa tsa lefutso le libopeho tsa mefuta e fapaneng.Taeo ena e ikemiselitse ho senola phetoho ea mefuta, ts'ebetso ea liphatsa tsa lefutso, mokhoa oa taolo ea liphatsa tsa lefutso maemong a genome ka ho tsebahatsa libopeho tsa tatelano le likarolo tse bolokileng kapa tse arohanyang mefuta e fapaneng.Boithuto bo tloaelehileng ba ho bapisa liphatsa tsa lefutso bo kenyelletsa tlhahlobo ea liphatsa tsa lefutso, nts'etsopele ea ho iphetola ha lintho, phetiso e felletseng ea genome, khatello ea khetho, jj.
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Bulked Segregant analysis
Bulked segregant analysis (BSA) ke mokhoa o sebelisoang ho tsebahatsa matšoao a lefutso a amanang le phenotype.Ts'ebetso ea mantlha ea BSA e kenyelletsa lihlopha tse peli tsa batho ba nang le phenotypes tse hanyetsanang haholo, ba kopanya DNA ea batho bohle ho etsa bongata ba DNA, e khethollang tatellano e fapaneng lipakeng tsa matamo a mabeli.Mokhoa ona o sebelisitsoe haholo ho khetholla matšoao a lefutso a amanang haholo le liphatsa tsa lefutso tse lebisitsoeng ho liphatsa tsa lefutso tsa limela/phoofolo.
