mRNA-seq (NGS) – De novo

Ho latellana ha mRNA ho dumella ho hlahlojwa ha dingolwa tsohle tsa mRNA diseleng tlasa maemo a itseng mme ke theknoloji e sebediswang haholo dibakeng tse fapaneng tsa dipatlisiso. Phaephe ya BMKCloud De novo mRNA-seq e etseditswe ho sekaseka dilaeborari tsa ho latellana tse ruisitsweng tsa poly-A ha ho se na genome ya referense e fumanehang. Phaephe e qala ka taolo ya boleng, e latelwe kede novoKopano ea mongolo le khetho ea sete ea unigene. Tlhahlobo ea sebopeho sa unigene e bolela esale pele tatellano ea ho ngola khoutu (CDS) le lipheta-pheto tse bonolo tsa tatellano (SSR). Ka mor'a moo, tlhahlobo ea polelo e fapaneng e fumana liphatsa tsa lefutso tse hlalositsoeng ka mokhoa o fapaneng (DEGs) har'a maemo a lekiloeng, e lateloa ke tlhaloso ea ts'ebetso le ntlafatso ea li-DEG ho ntša temohisiso ea baeloji.