Nyenyane ea RNA Sequencing-Illumina
Likaroloana
● Tokiso ea laebrari ea khetho ea boholo.
● Tlhahlobo ea bioinformatiki e itšetlehile ka ho bolela esale pele ka miRNA le lipakane.
Melemo ea Tšebeletso
●Tsebo e Pharalletseng: Re sebelitse lisampole tse fetang 300, tse nkang mefuta e fapaneng ea sampole. Re tlisa leruo la botsebi morerong o mong le o mong.
●Taolo ea Boleng e Tiileng: Re kenya tšebetsong lintlha tsa mantlha tsa taolo methating eohle, ho tloha ho tokiso ea sampole ho isa ho boitokiso ba laeborari, tatelano le bioinformatics. Tlhokomelo ea rona e hlokolosi e netefatsa ho fana ka liphetho tsa boleng bo holimo kamehla.
●Tlhahlobo e felletseng ea bioinformatics:E thusa ho tsebahatsa li-miRNA tse tsebahalang le tse ncha, ho tsebahatsa lipehelo tsa miRNAs, le litlhaloso tse tsamaellanang tsa ts'ebetso le ntlafatso ka li-database tse ngata (KEGG, GO).
●Tšehetso ea Post-Sales: Re utloisisa bohlokoa ba ho ba teng, ke ka lebaka leo boitlamo ba rona bo fetelang ka nģ'ane ho ho phethoa ha morero ka nako ea tšebeletso ea likhoeli tse 3 ka mor'a thekiso. Nakong ena, re fana ka tatellano ea projeke, thuso ea ho rarolla mathata, le linako tsa Q&A ho araba lipotso life kapa life tse amanang le sephetho.
Litlhoko tsa Mohlala le Phano
| Laebrari | Sethala | Lintlha tse khothalelitsoeng | Lintlha tsa QC |
| Boholo bo khethiloeng | Illumina SE50 | 10M-20M e baloa | Q30≥85% |
Litlhoko tsa Mohlala:
Li-nucleotides:
| Conc.(ng/μl) | Chelete (μg) | Bohloeki | Botšepehi |
| ≥80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Tšoaetso e fokolang kapa ha e na protheine kapa DNA e bontšitsoeng ho gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; bophahamo bo fokolang kapa bo se nang motheo |
● Limela:
Motso, Stem kapa Petal: 450 mg
Lekhasi kapa Peō: 300 mg
Litholoana: 1.2 g
● Phoofolo:
Pelo kapa mala: 450 mg
Viscera kapa Boko: 240 mg
mesifa: 600 mg
Masapo, Moriri kapa Letlalo: 1.5g
● Arthropods:
Likokoanyana: 9g
Crustacea: 450 mg
● Mali a feletseng: li-tubes tse 2
● Lisele: 106 lisele
● Serum le Plasma:6 ml
Tlhahiso ea Mohlala e khothalelitsoeng
Setshelo: 2 ml centrifuge tube (Tin foil ha e khothalletsoe)
Mohlala oa ho ngola: Sehlopha+katiso mohlala A1, A2, A3; B1, B2, B3.
Thomello:
1. Leqhoa le omileng: lisampole li lokela ho kenngoa ka mekotleng ebe li patoa ka leqhoa le omileng.
2. Li-tubes tsa RNAstable: lisampole tsa RNA li ka omisoa ka har'a tube ea ho tsitsisa ea RNA (mohlala, RNAstable®) le ho romelloa ka mocheso oa kamore.
Phallo ea Mosebetsi oa Ts'ebeletso
Moqapi oa liteko
Mohlala oa thomello
Tlhahiso ea RNA
Kaho ea laebrari
Tatelano
Tlhahlobo ea lintlha
Litšebeletso tsa ka mor'a thekiso
Bioinformatics
● Taolo ea boleng ba data e tala
● Sehlopha se senyenyane sa RNA
● Polelo ea miRNA le tlhahlobo ea polelo e fapaneng
● miRNA target gene le tlhaloso e fapaneng ea miRNA target gene annotation
● Keletso ea liphatsa tsa lefutso le ntlafatso ea ts'ebetso ea liphatsa tsa lefutso tsa miRNA ka tsela e fapaneng
Ho tsebahatsa miRNA: sebopeho le botebo
Pontšo e fapaneng ea miRNA - lihlopha tsa hiearchical
Tlhaloso ea ts'ebetso ea sepheo sa li-miRNA tse hlalositsoeng ka mokhoa o fapaneng
Lekola tsoelopele ea lipatlisiso e tsamaisoang ke lits'ebeletso tsa tatellano ea BMKGene'sRNA ka pokello e hlophisitsoeng ea lingoliloeng.
Chen, H. et al. (2023) 'Mafu a tšoaetso ea kokoana-hloko a thibela saponin biosynthesis le photosynthesis ho Panax notoginseng',Semela Physiology le Biochemistry, 203, leq. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) ' Semela sa FYVE se nang le protheine FREE1 se amahanngoa le likaroloana tsa microprocessor ho hatella miRNA biogenesis ',EMBO e tlaleha, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. le ba bang. (2023) 'MicroRNA Ame-Bantam-3p e Laola Nts'etsopele ea Larval Pupal ka ho Taisisa Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) ho Honeybee, Apis mellifera',International Journal of Molecular Sciences, 24(6), leq. 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al. (2018) 'Analysis e Kopanetsoeng ea MiRNA le Genes e Amanang le Boleng ba Nama e Senola hore Gga-MiR-140-5p e Ama Mafura a Intramuscular ka Likhoho',Cellular Physiology le Biochemistry, 46(6), leqepheng la 2421–2433. doi: 10.1159/000489649.
