Ho Latelana ha RNA e Nyenyane-Illumina
Likaroloana
● Tokisetso ea laeborari ea khetho ea boholo.
● Tlhahlobo ea Bioinformatic e shebane le ho bolela esale pele ha miRNA le lipheo.
Melemo ea Tšebeletso
●Bokgoni bo Pharaletseng: Re sebelitse disampole tse fetang 300, tse akaretsang mefuta e fapaneng ea disampole. Re tlisa tsebo e ngata morerong o mong le o mong.
●Taolo e Matla ea Boleng: Re kenya tshebetsong dintlha tsa taolo ya motheo mehatong yohle, ho tloha ho lokiseng mehlala ho isa ho lokiseng laeborari, tatellano le bioinformatics. Tlhokomelo ya rona e hlokolosi e netefatsa hore ho tla ba le diphetho tsa boleng bo hodimo ka dinako tsohle.
●Tlhahlobo e felletseng ea bioinformatics:E nolofalletsa ho tsebahatsa li-miRNA tse tsejoang le tse ncha, ho tsebahatsa lipheo tsa li-miRNA, le tlhaloso e sebetsang e tsamaellanang le ntlafatso ka li-database tse ngata (KEGG, GO).
●Tšehetso ea Kamora Thekiso: Rea utloisisa hore na ho ba teng ho bohlokoa hakae, ke ka lebaka leo boitlamo ba rona bo fetang ho phethela projeke ka nako ea tšebeletso ea likhoeli tse 3 kamora thekiso. Nakong ena, re fana ka tlhahlobo ea projeke, thuso ea ho rarolla mathata, le lipuisano tsa lipotso le likarabo ho araba lipotso life kapa life tse amanang le liphetho.
Litlhoko tsa Sampole le Phano
| Laeborari | Sethala | Lintlha tse khothaletsoang | QC ea Lintlha |
| Boholo bo khethiloe | Illumina SE50 | Lipalo tse 10M-20M | Q30≥85% |
Litlhoko tsa Sampole:
Li-nucleotide:
| Qetello(ng/μl) | Bongata (μg) | Bohloeki | Botšepehi |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Tšilafalo ea protheine kapa DNA e lekanyelitsoe kapa ha e eo ho gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; bophahamo ba motheo bo lekanyelitsoeng kapa ha bo na letho |
● Limela:
Motso, Kutu kapa Lehlaku: 450 mg
Lekhasi kapa Peo: 300 mg
Litholoana: 1.2 g
● Phoofolo:
Pelo kapa Mala: 450 mg
Viscera kapa Boko: 240 mg
Mesifa: 600 mg
Masapo, Moriri kapa Letlalo: 1.5g
● Li-arthropod:
Likokoanyana: 9g
Sekotwana sa crustacea: 450 mg
● Mali a felletseng: Liphaephe tse 2
● Lisele: 106 lisele
● Serum le Plasma:6 mL
Thomello e Khothaletsoang ea Sampole
Setshelo: 2 ml tube ea centrifuge (Ha ho kgothaletswe ho kenya tin foil)
Ho ngola dileiboleng tsa mohlala: Sehlopha + kopi mohlala A1, A2, A3; B1, B2, B3.
Thomello:
1. Leqhoa le omileng: Disampole di lokela ho pakwa ka mekotleng mme di patwe ka hara leqhoa le omileng.
2. Liphaephe tse tsitsitseng tsa RNA: Disampole tsa RNA di ka omiswa ka hara phaephe e tsitsitseng ya RNA (mohlala, RNAstable®) mme tsa romelwa mochesong wa kamore.
Phallo ea Mosebetsi oa Tšebeletso
Moralo oa liteko
Phano ea mohlala
Ho ntšoa ha RNA
Kaho ea laeborari
Tatellano
Tlhahlobo ea lintlha
Litšebeletso tsa kamora thekiso
Litsebi tsa Bioinformatics
● Taolo ea boleng ba data e tala
● Sehlopha sa RNA e nyane
● tlhahlobo ea polelo ea miRNA le tlhaloso e fapaneng
● gene e reretsoeng miRNA le tlhaloso ea gene e reretsoeng miRNA e hlahisitsoeng ka tsela e fapaneng
● Tlhaloso ea liphatsa tsa lefutso tse reretsoeng le ntlafatso ea mosebetsi oa liphatsa tsa lefutso tsa miRNA tse hlalositsoeng ka tsela e fapaneng
Tlhaloso ea miRNA: sebopeho le botebo
Tlhaloso e fapaneng ea miRNA - ho bokellana ha lihlopha ka tatellano ea maemo
Tlhaloso e sebetsang ea sepheo sa li-miRNA tse hlahisoang ka tsela e fapaneng
Hlahloba tsoelo-pele ea lipatlisiso e tsamaisoang ke lits'ebeletso tsa tatellano ea sRNA tsa BMKGene ka pokello ea lingoliloeng tse hlophisitsoeng hantle.
Chen, H. et al. (2023) 'Tšoaetso ea vaerase e thibela saponin biosynthesis le photosynthesis ho Panax notoginseng',Fisioloji ea Limela le Biokhemistri, 203, leq. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) 'Semela se nang le protheine ea FYVE FREE1 se amahanya le likarolo tsa microprocessor ho thibela biogenesis ea miRNA',Litlaleho tsa EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'MicroRNA Ame-Bantam-3p e Laola Nts'etsopele ea Li-Larval Pupal ka ho Shebana le Li-Domains tse ngata tsa Epidermal Growth-like Domains 8 Gene (megf8) ho Honeybee, Apis mellifera',Koranta ea Machaba ea Saense ea Limolek'hule, 24(6), leq. 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al. (2018) 'Tlhahlobo e Kopantsoeng ea MiRNA le Liphatsa tsa lefutso tse Amanang le Boleng ba Nama e Senola Hore Gga-MiR-140-5p e Ama ho Kenngoa ha Mafura ka Litšusumetsong tsa 'Mele ho Likhoho',Fisioloji ea Lisele le Biokhemistri, 46(6), leqepheng la 2421–2433. doi: 10.1159/000489649.
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