Super Lowest Price Transcriptome Sequencing Service - Eukaryotic mRNA sequencing – Illumina – Biomarker
Super Lowest Price Transcriptome Sequencing Service - Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
| Library | Sequencing strategy | Data recommended | Quality Control |
| Poly A enriched | Illumina PE150 |
6 Gb |
Q30≥85% |
Sample Requirements:
Nucleotides:
| Purity | Integrity | Amount |
| OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. | For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation | Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
We rely upon strategic thinking, constant modernisation in all segments, technological advances and of course upon our employees that directly participate inside our success for Super Lowest Price Transcriptome Sequencing Service - Eukaryotic mRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Greenland , Paris , Poland , We aim to build a famous brand which can influence a certain group of people and light up the whole world. We want our staff to realize self-reliance, then achieve financial freedom, lastly obtain time and spiritual freedom. We do not focus on how much fortune we can make, instead we aim to obtain high reputation and be recognized for our products. As a result, our happiness comes from our clients satisfaction rather than how much money we earn. Ours team will do best for you always.
By Ivy
from Rotterdam
- 2017.03.28 16:34
The goods we received and the sample sales staff display to us have the same quality, it is really a creditable manufacturer.
By Frances
from Switzerland
- 2017.04.08 14:55
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