Trending Products Bisulfite Sequencing - Eukaryotic mRNA sequencing – Illumina – Biomarker
Trending Products Bisulfite Sequencing - Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
| Library | Sequencing strategy | Data recommended | Quality Control |
| Poly A enriched | Illumina PE150 |
6 Gb |
Q30≥85% |
Sample Requirements:
Nucleotides:
| Purity | Integrity | Amount |
| OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. | For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation | Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
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Our team through qualified training. Skilled professional knowledge, powerful sense of support, to satisfy the support desires of consumers for Trending Products Bisulfite Sequencing - Eukaryotic mRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Cologne , Chicago , Luxembourg , Our company's main products are widely used all over the world; 80% of our products exported to the United States, Japan, Europe and other markets. All stuff sincerely welcome guests come to visit our factory.
By Susan
from Turkey
- 2017.12.09 14:01
This is a very professional and honest Chinese supplier, from now on we fell in love with the Chinese manufacturing.
By Ann
from Sudan
- 2018.03.03 13:09
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