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条形 ibhanile-03

Iimveliso

I-Assay ye-Chromatin eFikelelekayo yeTransposase kunye noSetyenziso lwe-High throughput (ATAC-seq)

Uvavanyo lweChromatin eFumanekayo-Transposase ene-High throughput Sequencing (ATAC-seq) Umfanekiso ofakiweyo
  • I-Assay ye-Chromatin eFikelelekayo yeTransposase kunye noSetyenziso lwe-High throughput (ATAC-seq)
  • I-Assay ye-Chromatin eFikelelekayo yeTransposase kunye noSetyenziso lwe-High throughput (ATAC-seq)
  • I-Assay ye-Chromatin eFikelelekayo yeTransposase kunye noSetyenziso lwe-High throughput (ATAC-seq)
  • I-Assay ye-Chromatin eFikelelekayo yeTransposase kunye noSetyenziso lwe-High throughput (ATAC-seq)
  • I-Assay ye-Chromatin eFikelelekayo yeTransposase kunye noSetyenziso lwe-High throughput (ATAC-seq)

I-ATAC-seq yindlela ephezulu yokulandelelanisa i-genome-wide-wide chromatin accessibility, ebaluleke kakhulu kulawulo lwe-epigenetic lwehlabathi jikelele lwe-gene expression.Iiadaptha zolandelelwano zifakwe kwimimandla yechromatin evulekileyo nge-hyperactive Tn5 transposase.Emva kokwandiswa kwePCR, ithala leencwadi lolandelelwano liyakhiwa.Yonke imimandla ye-chromatin evulekileyo inokufumaneka phantsi kwemeko ethile yexesha lesithuba, ingaphelelanga kuphela kwiindawo ezibophelelayo ze-transcription factor, okanye ummandla othile we-histone modified.


Iinkcukacha zeNkonzo

Bioinformatics

Iziphumo zeDemo

Iinkonzo eziluncedo

● Uhlalutyo oluBanzi: Umbhobho wokuhlalutya kobuchwephesha ukuhlangabezana neemfuno ezahlukeneyo zokuhlalutya ngokwezifiso;

● Amava aBanzi: Amakhulu eeprojekthi agqitywe ngempumelelo ukuza kuthi ga ngoku, kubandakanywa iisampuli zezilwanyana nezityalo.

IiNgcaciso zeNkonzo

Iqonga: Illumina NovaSeq iqonga

Uhlobo lwethala leencwadi: ATAC-seq

Imveliso yedatha ecetyiswayo: ≥20M iyafundwa

IiMfuno zeSampulu

Uhlobo lwesampulu: Izicubu, iiseli eziphilayo, okanye iiseli ezikhenkcezisiweyo njalo njalo

Inombolo yeSeli: ≥ 5×105iiseli, inombolo yeseli echanekileyo ngundoqo kwimpumelelo yolingelo;

Ubunzima bezicubu: ≥ 200mg izicubu ezitsha;

Igazi: ≥ 2 mL

Ukuhamba komsebenzi weNkonzo

Isampulu ye-QC

Uyilo lovavanyo

ukuhanjiswa kwesampuli

Ukuhanjiswa kwesampuli

Ulingo lokulinga

Ukukhutshwa kwe-DNA

ULungiselelo lweThala leeNcwadi

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Ukulandelelanisa

Uhlalutyo lwedatha

Uhlalutyo lwedatha

Emva kweeNkonzo zentengiso

Iinkonzo emva kokuthengisa

  • Ngaphambili:
  • Okulandelayo:

    • 表观-02

    I-1.I-Heatmap kwi-ATAC ifundeka ngokusasazwa kwi-TSS nakwimimandla ekufutshane (±3 kb)

    3 (1)2.Ukusasazwa kwendawo evulekileyo yechromatin kumacandelo ahlukeneyo ejenome

    4 (1)

    3.Incopho yokufowuna phakathi kwamaqela

    5 (1)

    fumana isicatshulwa

    Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    Imvelisoiindidi

    Thumela umyalezo wakho kuthi:

    Cofa u-enter ukukhangela okanye i-ESC ukuvala