Iimveliso

Iintsholongwane ziGqibezela iGenome

Ibhaktheriya Gqibezela iGenome Umfanekiso obonakalayo

I-Biomarker Technologies ibonelela ngenkonzo yolandelelwano ekwakheni i-genome epheleleyo yebhaktiriya ene-gap engu-zero.Ukuhamba komsebenzi okuphambili kwebhaktheriya ukwakhiwa kwegenome okupheleleyo kubandakanya ulandelelwano lwesizukulwana sesithathu, ukudibanisa, inkcazo esebenzayo kunye nohlalutyo oluphambili lwe-bioinformatic oluzalisekisa iinjongo ezithile zophando.Iprofayili ebanzi ngakumbi yeebhaktiriya genome ixhobisa ukutyhilwa kweendlela ezisisiseko ezisisiseko senkqubo yazo yebhayoloji, enokubonelela ngereferensi ebalulekileyo kuphando lwegenomic kwiintlobo eziphezulu ze-eukaryotic.

Iqonga:Nanopore PromethION P48 + Illumina NovaSeq Platform

I-PacBio Sequel II

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Iziphumo zeDemo

Iinkonzo eziluncedo

● Izicwangciso ezininzi zolandelelwano ezikhoyo kwiinjongo zophando ezahlukeneyo

● Ibhaktheriya epheleleyo igenome ene-0 Gap eqinisekisiwe.

● Unamava kakhulu ekudityanisweni kwegenome yebhaktiriya ene-genome engaphezu kwe-10,000 epheleleyo edityanisiweyo.

● Iqela lenkxaso yobugcisa emva kokuthengiswa lizalisekisa iimfuno ezithe ngqo zophando.

IiNgcaciso zeNkonzo

UkulandelelanisaIsicwangciso

Ithala leencwadi

Umgangatho uqinisekisiwe

Ixesha eliqikelelweyo lokujika

Nanopore 100X + Illumina 50X

Nanopore 20K

PE150

0 Umsantsa

Iintsuku ezingama-30

I-PacBio HiFi 30X

I-PacBio 10K

Uhlalutyo lwe-Bioinformatics

● Ulawulo lomgangatho wedatha ekrwada

● Indibano yeGenome

● Uhlalutyo lwecandelo leGenome

● Inkcazo yomsebenzi wemfuza

● Uhlalutyo lokuthelekisa i-genomic

IiMfuno zeSampulu kunye nokuhanjiswa

IiMfuno zeSampulu:

KubaIzicatshulwa zeDNA:

Uhlobo lweSampuli	Isixa	Ukugxininisa	Ubunyulu
Izicatshulwa zeDNA	＞ 2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Ngeisampulu zethishu:

Uhlobo lwesampuli

Unyango olucetyiswayo lwesampulu

Ukugcinwa kwesampuli kunye nokuthunyelwa

Iintsholongwane

Jonga iibhaktheriya phantsi kwe-microscope kwaye uqokelele ibhaktheriya kwisigaba sazo sokwandisa

Dlulisa inkcubeko yebhaktheriya (equlethe malunga neeseli ze-3-4.5e9) kwi-1.5 okanye i-2 ml eppendorf.(Gcina emkhenkceni)

I-Centrifuge ityhubhu ye-1 min kwi-14000 g ukuqokelela ibhaktheriya kunye nokususa i-supernatant ngononophelo.

Vala ityhubhu kwaye ukhenkce iibhaktheriya kwi-nitrogen engamanzi ubuncinane ubuncinane be-30 min.Gcina ityhubhu kwi -80 ℃ efrijini.

Umkhenkce iisampulu kulwelo nitrogen iiyure 3-4 kwaye ugcine kulwelo nitrogen okanye -80 degree ukuya ugcino ixesha elide.Isampulu yokuthumela ngenqanawe eyomileyo iyafuneka.