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Iimveliso

IBacterial kunye neFungal Whole Genome Re-Sequencing

I-Bacterial kunye neFungal Whole Genome Ukulandelelanisa kwakhona umfanekiso obonakalayo

Ukulandelelana kwakhona kwejenome yebhaktiriya kunye nomngundo sisixhobo esibalulekileyo sokugqiba iigenomes zebhaktiriya eyaziwayo kunye nomngundo, kunye nokuthelekisa iigenomes ezininzi okanye ukwenza imephu yejenomi yezinto ezitsha.Kubaluleke kakhulu ukulandelelanisa iigenomes zonke zebhaktiriya kunye nomngundo ukuze kuveliswe iigenome zesalathiso ezichanekileyo, ukuchongwa kwe-microbial kunye nolunye uphononongo lothelekiso lwe-genome.

Iqonga: Iqonga le-Illumina NovaSeq

Qhagamshelana nathi

Iinkcukacha zeNkonzo

IiNgcaciso zeNkonzo

Iqonga: Iqonga leNovaSeq 6000, PE150

Uhlobo lwethala leencwadi: 350bp faka ithala leencwadi le-cDNA (kuxhomekeke kwincopho yosasazo)

Isicwangciso sokulandelelana: I-Paired-End 150 bp

Cebisa ulandelelwano lugubungela: ≥100×

IiMfuno zeSampulu

Isixa seGenomic DNA:≥ 3ug

IGenomic DNA Concentration:≥ 20ng/μL

Ukuhamba komsebenzi weNkonzo

ukuhanjiswa kwesampuli

Ukuhanjiswa kwesampuli

ULungiselelo lweThala leeNcwadi

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Ukulandelelanisa

Uhlalutyo lwedatha

Uhlalutyo lwedatha

Emva kweeNkonzo zentengiso

Iinkonzo emva kokuthengisa

Ngaphambili: PacBio-Full-length 16S/18S/ITS Amplicon Sequencing

Okulandelayo: 16S/18S/ITS Amplicon Sequencing-NGS

fumana isicatshulwa

Bhala umyalezo wakho apha kwaye uwuthumele kuthi

Imvelisoiindidi

Thumela umyalezo wakho kuthi: