Iimveliso

I-BMKMANU S1000 i-Spatial Transcriptome

BMKMANU S1000 Spatial Transcriptome Featured Image

Ukulungelelaniswa kwendawo yeeseli kudlala indima ebalulekileyo kwiinkqubo ezahlukeneyo zebhayoloji, ezifana nokungena komzimba, ukuphuhliswa kwe-embryo, njl.Ngophuhliso lwetekhnoloji, i-ultra-clear-clear tissue morphology kunye nomahluko wokwenyani wesakhiwo sokubonakaliswa kwemolekyuli yendawo kufuneka ifundwe ngesisombululo esiphezulu.I-BMKGENE ibonelela ngenkonzo ebanzi, yokumisa enye yolandelelwano lwe-spatial transcriptome ukusuka kwiisampulu ukuya kwiimbono zebhayoloji.

Itekhnoloji ye-Spatial transcriptomics ixhobisa iimbono zenoveli kwibala lophando olwahlukeneyo ngokusombulula iprofayile yegenesis enomxholo wesithuba kwiisampulu ezingafaniyo.

Itshiphu ye-Spatial transcriptome: BMKMANU S1000

Iqonga: I-Illumina NovaSeq Platform

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Bioinformatics

Iziphumo zeDemo

BMKMANU S1000 Spatial Transcriptome Technical Scheme

Izinto ezilungileyo ze-BMKMANU S1000

1) Isisombululo se-Sub-cellular: Indawo nganye yokubanjwa iqulathe >2 yezigidi ezi-2 zeeNdawo ezineBarcoded ezisisithuba ezinobubanzi obuyi-2.5 µm kunye nesithuba se-5 µm phakathi kwamaziko amabala, okwenza ukuba uhlalutyo lwe-transcriptome lwesithuba lube nesisombululo se-sub-cellular (5 µm).

2) Uhlalutyo lwesisombululo samanqanaba amaninzi: Uhlalutyo oluguquguqukayo lwamanqanaba amaninzi ukusuka kwi-100 μm ukuya kwi-5 μm ukusombulula iimpawu zezicubu ezahlukeneyo kwisisombululo esisiso.

I-3) Iprofayili ye-transcriptome ebanzi: Imibhalo ethathwe kuyo yonke i-slide ye-tissue ingahlalutywa, ngaphandle kokuthintelwa kwinani leejene ezijoliswe kuyo kunye nommandla ojoliswe kuwo.

IiNgcaciso zeNkonzo

Ithala leencwadi	Isicwangciso sokulandelelana	Isiphumo seDatha siyacetyiswa
S1000 cDNA ilayibrari	BMKMANU S1000-Illumina PE150	I-60Gb / isampuli

IiMfuno zeSampulu

Isampulu	Inani	Ubungakanani	Umgangatho weRNA
Ibhloko yethishu efakwe kwi-OCT	2-3 iibhloko / isampuli	Malunga.6.8x6.8x6.8 mm³	I-RIN≥7

Ukufumana iinkcukacha ezithe vetshe malunga nesikhokelo sokulungiselela isampula kunye nokuhamba komsebenzi wenkonzo, nceda uzive ukhululekile ukuthetha nayeBMKGENE ingcaphephe

Ukuhamba komsebenzi weNkonzo

Uyilo lovavanyo

Ukuhanjiswa kwesampuli

Uvavanyo lwangaphambili

Ibarcodes yendawo

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Uhlalutyo lwedatha

Iinkonzo emva kokuthengisa

Ngaphambili: I-10x ye-Genomics yeVisium ye-Spatial Transcriptome

Okulandelayo: Ulandelelwano lweGenome yeSityalo/yeSilwanyana

Idatha eveliswe yi-BMKMANU S1000 ihlalutywa kusetyenziswa i-software "BSTMatrix", eyilwe ngokuzimeleyo yi-BMKGENE, kuquka:

1) Ukuveliswa kwe-matrix ye-Gene expression
2) Ukulungiswa komfanekiso we-HE
3) Iyahambelana nesoftware yomntu wesithathu esezantsi yokuhlalutya
4) Kwi-Intanethi "i-BSTViewer" inceda ukufumana iziphumo zokubonwayo kwizisombululo ezahlukeneyo.

1.Spot clustering

I-s1000 (4)