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Iimveliso

Ulandelelwano lweChromatin Immunoprecipitation (ChIP-seq)

Umfanekiso obonisiweyo weChromatin Immunoprecipitation Sequencing (ChIP-seq)

I-Chromatin Immunoprecipitation (CHIP) yindlela esebenzisa ii-antibodies ukuze ityebise ngokukhethekileyo iiproteni ezibopha i-DNA kunye neethagethi zazo ze-genomics ezihambelanayo. Ukudibana kwayo ne-NGS kwenza ukuba iprofayili ye-genome ngokubanzi yeethagethi ze-DNA ezinxulumene nokuguqulwa kwe-histone, izinto ezibangela ukubhalwa kwe-transcription, kunye nezinye iiproteni ezibopha i-DNA. Le ndlela inamandla ivumela ukuthelekiswa kweendawo zokubopha kwiintlobo ezahlukeneyo zeeseli, izicubu, okanye iimeko. Ukusetyenziswa kwe-ChIP-Seq kuqala ekufundeni ummiselo wokubhalwa kwe-transcription kunye neendlela zophuhliso ukuya ekucaciseni iindlela zesifo, okwenza kube sisixhobo esibalulekileyo sokuqonda iindawo zommiselo we-genomic kunye nokuqhubela phambili ukuqonda ngonyango.

Iqonga: Illumina NovaSeq

Qhagamshelana nathi

Iinkcukacha zeNkonzo

I-Bioinformatics

Iziphumo zeDemo

Iingenelo zeNkonzo

●Uhlalutyo oluPhambili lweBioinformatiki kunye neNkcazo eNzulu:Sisebenzisa iidathabheyisi ezininzi ukuze sichaze ngokusebenzayo iijini ezinxulumene neendawo zokubopha iproteni-DNA, sinike ulwazi malunga neenkqubo zeseli kunye neemolekyuli ezisisiseko sokusebenzisana.

●Inkxaso emva kokuthengisa:Ukuzibophelela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye nexesha leenkonzo emva kokuthengiswa kweenyanga ezi-3. Ngeli xesha, sinikezela ngokulandela iprojekthi, uncedo lokusombulula iingxaki, kunye neeseshoni ze-Q&A ukuphendula nayiphi na imibuzo enxulumene neziphumo.

●Amava amaninzi:Njengoko inkampani yethu inerekhodi yokugqiba ngempumelelo iiprojekthi ezininzi zeChIP-Seq, izisa ubuchule obungaphezulu kweminyaka elishumi etafileni. Iqela lethu lohlalutyo elinobuchule obuphezulu, kunye nomxholo opheleleyo kunye nenkxaso emva kokuthengisa, liqinisekisa impumelelo yeeprojekthi zakho.

● Ulawulo Oluqinileyo Lomgangatho: Sisebenzisa amanqaku olawulo oluphambili kuzo zonke izigaba, ukusuka ekulungiseleleni iisampulu kunye nethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics. Olu hlolo olucokisekileyo luqinisekisa ukunikezelwa kweziphumo ezisemgangathweni ophezulu rhoqo.

Iinkcukacha zeNkonzo

Ithala leeNcwadi	Icebo Lokulandelelanisa	Imveliso yedatha ecetyiswayo	Ulawulo lwemeko
I-DNA ecocekileyo emva kwe-Immunoprecipitation	I-Illumina PE150	10Gb	Q30≥85%