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I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

I-Chromatin Immunoprecipitation Sequencing (i-ChIP-seq) Umfanekiso okhoyo

I-Chromatin Immunoprecipitation (CHIP) bubuchule obusebenzisa izilwa-buhlungu ukuba zityebise ngokukhetha iiproteni ezibophelela i-DNA kunye neethagethi zazo ezihambelana ne-genomics. Ukuhlanganiswa kwayo kunye ne-NGS kwenza ukuba i-genome-wide profiling yeethagethi ze-DNA ezinxulumene nokuguqulwa kwe-histone, izinto ezibhaliweyo, kunye nezinye iiprotheni ezibophelelayo ze-DNA. Le ndlela iguquguqukayo yenza uthelekiso lweendawo ezibophelelayo kuzo zonke iintlobo zeeseli ezahlukeneyo, izicubu, okanye iimeko. Izicelo ze-ChIP-Seq zisusela ekufundeni umgaqo woshicilelo kunye neendlela zophuhliso ukuya ekucaciseni iindlela zesifo, oko kuyenza ibe sisixhobo esibalulekileyo sokuqonda ubume bolawulo lwe-genomic kunye nokuqhubela phambili kweembono zonyango.

Iqonga: Illumina NovaSeq

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Bioinformatics

Iziphumo zeDemo

Iinkonzo eziluncedo

●Uhlalutyo oluPhezulu lwe-Bioinformatic kunye ne-Comprehensive Annotation:Sisebenzisa ii-database ezininzi ukuze sichaze ngokusebenzayo iijene ezihambelana nemimandla yeprotheyini-DNA ebophelelayo, inikezela ngengqiqo kwiinkqubo zeselula kunye neemolekyuli eziphantsi kwentsebenziswano.

●Inkxaso yasemva kwentengiso:Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.

●Amava Abanzi:Ngerekhodi yokugqiba ngempumelelo iiprojekthi ezininzi ze-ChIP-Seq, inkampani yethu izisa ngaphezulu kweminyaka elishumi yobuchule etafileni. Iqela lethu lohlalutyo lwezakhono eziphezulu, lidityaniswa nomxholo obanzi kunye nenkxaso yasemva kokuthengisa, iqinisekisa impumelelo yeeprojekthi zakho.

● ULawulo loMgangatho olungqongqo: Sisebenzisa iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kwisampulu kunye nokulungiswa kwethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics. Olu hlolo lucokisekileyo luqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.

IiNgcaciso zeNkonzo

Ithala leencwadi	Isicwangciso solandelelwano	Imveliso yedatha ecetyiswayo	Ulawulo lwemeko
I-DNA ecocekileyo emva kwe-Immunoprecipitation	Illumina PE150	I-10Gb	Q30≥85%