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条形 ibhanile-03

Iimveliso

I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

I-Chromatin Immunoprecipitation Sequencing (I-ChIP-seq) Umfanekiso obonakalayo
  • I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)
  • I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

I-ChIP-Seq inikezela ngeprofayili ye-genome-wide-wide ye-DNA ekujoliswe kuyo yokuguqulwa kwe-histone, izinto ezibhaliweyo, kunye nezinye iiprotheni ezinxulumene ne-DNA.Idibanisa ukukhethwa kwe-chromatin immuno-precipitation (i-ChIP) ukubuyisela i-protein-DNA complexes ethile, kunye namandla okulandelelanisa isizukulwana esilandelayo (NGS) ukulandelelana kwe-high-throughput ye-DNA efunyenweyo.Ukongeza, ngenxa yokuba iiprotheyini ze-DNA zifunyenwe kwiiseli eziphilayo, iindawo zokubopha zinokuthelekiswa kwiintlobo zeeseli ezahlukeneyo kunye nezicubu, okanye phantsi kweemeko ezahlukeneyo.Izicelo zisusela kummiselo wokubhaliweyo ukuya kwiindlela zophuhliso kwiindlela zokugula nangaphaya.

Iqonga: I-Illumina NovaSeq Platform


Iinkcukacha zeNkonzo

Bioinformatics

Iziphumo zeDemo

Iinkonzo eziluncedo

● Iqonga: I-Illumina NovaSeq Platform, PE150

● Uhlobo lwethala leencwadi: 100-500bp faka ithala leencwadi leDNA (kuxhomekeke kwincopho yosasazo)

● Isicwangciso sokulandelelana: I-Paired-End 150 bp

● Cebisa imveliso yedatha: 6 Gb idatha eluhlaza / isampuli.

IiMfuno zeSampulu

Ubuninzi beDNA: ≥ 70 ng, incopho ephambili ye-100 ukuya ku-500 bp

Umthamo wesampuli: ≥ 10 μl

I-OD260/280 = 1.8 ukuya kwi-2.0 ngaphandle kokuthotywa okanye ukungcoliswa kwe-RNA

Ukuqhutywa komsebenzi wokulinga

图片1

Ukuhamba komsebenzi weNkonzo

ukuhanjiswa kwesampuli

Ukuhanjiswa kwesampuli

ULungiselelo lweThala leeNcwadi

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Ukulandelelanisa

Uhlalutyo lwedatha

Uhlalutyo lwedatha

Emva kweeNkonzo zentengiso

Iinkonzo emva kokuthengisa

  • Ngaphambili:
  • Okulandelayo:

    • 表观-01

    1. Incopho yosasazo lokutyebisa

    官网图1

     

    2. Uyilo lwe-MA lwencopho yokwahlukana

    官网图2

     

    3.Inkcazo ye-KEGG

    官网图3

     

    4. GO ukuhlelwa

    官网图4

    fumana isicatshulwa

    Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    Imvelisoiindidi

    Thumela umyalezo wakho kuthi:

    Cofa u-enter ukukhangela okanye i-ESC ukuvala