circRNA ulandelelwano-Illumina
Iimbonakalo
● Ukuncipha kwe-rRNA okulandelwa lulungiselelo lwethala leencwadi eliqondisayo, okwenza kube lula ukulandelelana kwedatha ethe ngqo kwi-strand.
● I-Bioinformamatic workflow yenza ingqikelelo ye-circRNA kunye nenkcazo yobungakanani
Iinkonzo eziluncedo
●Amathala eencwadi e-RNA abanzi:sisebenzisa i-rRNA depletion endaweni yokunciphisa i-RNA yomgca kulungiselelo lwethu lwangaphambili lwelayibrari, siqinisekisa ukuba idatha yolandelelwano ibandakanya i-circRNA kuphela kodwa i-mRNA kunye ne-lncRNA, eyenza uhlalutyo oludibeneyo kwezi datha.
●Uhlalutyo olukhethwayo lothungelwano lwe-RNA (ceRNA) olukhuphisanayo: Ukubonelela ngengqiqo enzulu kwiindlela zokulawula iiselula
●UbuGcisa obuBanzi: ngerekhodi yokusetyenzwa kweesampulu ezingaphezu kwama-20,000 kwi-BMK, ethatha iintlobo ngeentlobo zeesampulu kunye neeprojekthi ze-lncRNA, iqela lethu lizisa ubutyebi bamava kwiprojekthi nganye.
●Ulawulo loMgangatho olungqongqo: siphumeza iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kwisampulu kunye nolungiselelo lwethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics.Olu hlolo lucokisekileyo luqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.
● Inkxaso yePost-Sales: Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo.Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno zeSampulu kunye nokuhanjiswa
| Ithala leencwadi | Iqonga | Idatha ecetyiswayo | Idatha ye-QC |
| UPoly A utyebile | Illumina PE150 | 16-20 Gb | Q30≥85% |
IiMfuno zeSampulu:
IiNucleotides:
| Conc.(ng/μl) | Imali (μg) | Ubunyulu | Ingqibelelo |
| ≥ 100 | ≥ 0.5 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | Kwizityalo: RIN≥6.5; Kwizilwanyana: RIN≥7.0; 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
● Izityalo:
Ingcambu, Isiqu okanye iPetal: 450 mg
Igqabi okanye Imbewu: 300 mg
Isiqhamo: 1.2 g
● Isilwanyana:
Intliziyo okanye Amathumbu: 450 mg
Viscera okanye Brain: 240 mg
Izihlunu: 600 mg
Amathambo, Iinwele okanye Ulusu: 1.5g
● IiArthropods:
Izinambuzane: 9g
I-crustacea: 450 mg
● Igazi elipheleleyo:2 iityhubhu
● Iiseli: 106 iiseli
● ISerum kunye nePlasmayena: 6 ml
Unikezelo lweSampuli eCetyisiweyo
Isikhongozeli: 2 ml centrifuge ityhubhu (Ifoyile yeTin ayikhuthazwa)
Isampuli yokuleyibhela: Iqela+liphindaphinda umz. A1, A2, A3;B1, B2, B3... ...
Ukuthunyelwa ngenqanawa:
1. Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa kwaye zingcwatywe kumkhenkce owomileyo.
2. Iityhubhu ze-RNAstable: Iisampulu ze-RNA zingomiswa kwi-tube ye-RNA yokuzinzisa (umzekelo, i-RNAstable®) kwaye ithunyelwe kwiqondo lokushisa.
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukukhutshwa kwe-RNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Bioinformatics
uqikelelo lwe-circRNA: usasazo lwechromosomal
Ii-circRNA ezibonakaliswe ngokwahlukileyo-isakhiwo se-Volcano
Ii-circRNAs ezibonakaliswe ngokwahlukileyo – ukudityaniswa ngokwemigangatho
Ukutyetyiswa okusebenzayo kweejene ze-circRNA's host
Phonononga inkqubela phambili yophando eququzelelwa yi-BMKGene'inkonzo zolandelelwano lwe-circRNA ngengqokelela egcinwe yopapasho.
Wang, X. et al.(2021) 'i-CPSF4 ilawula ukubunjwa kwe-circRNA kunye ne-microRNA mediated gene silence in hepatocellular carcinoma', i-Oncogene 2021 40: 25, 40 (25), iphe. 4338-4351.doi: 10.1038/s41388-021-01867-6.
Xia, K. et al.(2023) 'i-X oo-responsive transcriptome ibonisa indima ye-RNA133 yesetyhula ekuxhathiseni izifo ngokulawula ukubonakaliswa kwe-OsARAB kwirayisi', iPhytopathology Research, 5 (1), iphe. 1-14.doi: 10.1186/S42483-023-00188-8/AMAFANI/6.
Y, H. et al.(2023) 'i-CPSF3 imodareyitha ibhalansi yeesetyhula kunye nemigca yemigca kwi-hepatocellular carcinoma'.doi: 10.21203/RS.3.RS-2418311/V1.
Zhang, Y. et al.(2023) 'Uvavanyo olupheleleyo lwe-circRNAs kwi-cardiomyopathy ye-cirrhotic ngaphambi nangemva kokutshintshwa kwesibindi', i-International Immunopharmacology, i-114, iphe.109495. doi: 10.1016/J.INTIMP.2022.109495.
