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Iimveliso

Iilayibrari ezenziwe kwangaphambili ze-DNBSEQ

I-DNBSEQ, eyaphuhliswa yi-MGI, yitekhnoloji entsha ye-NGS eye yakwazi ukunciphisa iindleko zokulandelelana nokunyusa umthamo. Ukulungiswa kweelayibrari ze-DNBSEQ kubandakanya ukuqhekeka kwe-DNA, ukulungiswa kwe-ssDNA, kunye nokwandiswa kwesangqa esijikelezayo ukuze kufunyanwe ii-nanoballs ze-DNA (DNB). Ezi emva koko zilayishwa kumphezulu oqinileyo kwaye emva koko zilandelelaniswe yi-combinatorial Probe-Anchor Synthesis (cPAS). Itekhnoloji ye-DNBSEQ idibanisa iingenelo zokuba nesantya esiphantsi sempazamo yokukhulisa kunye nokusebenzisa iipateni zempazamo ezinoxinano oluphezulu kunye nee-nanoballs, nto leyo ekhokelela kulandelelwano olune-throughput ephezulu kunye nokuchaneka.

Inkonzo yethu yokulandelelanisa iilayibrari eyenziwe kwangaphambili ivumela abathengi ukuba balungiselele iilayibrari zokulandelelanisa ii-Illumina ezivela kwimithombo eyahlukeneyo (i-mRNA, i-genome epheleleyo, i-amplicon, iilayibrari ezili-10x, phakathi kwezinye), eziguqulwa zibe ziilayibrari ze-MGI kwiilabhoratri zethu ukuze zilandelelaniswe kwi-DNBSEQ-T7, nto leyo evumela ukuba kubekho idatha ephezulu ngeendleko eziphantsi.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Isiphumo seDemo

Iimbonakalo

●Iqonga:I-MGI-DNBSEQ-T7

●Iindlela zokulandelelanisa:PE150

●Ukudluliselwa kweelayibrari ze-IlluminaI-MGI:okuvumela ukulandelelana kwenani eliphezulu ledatha ngexabiso eliphantsi.

●Ulawulo lomgangatho weelayibrari ngaphambi kokulandelelana.

●Ukulandelelanisa idatha ye-QC kunye nokuhanjiswa kwayo:ukuhanjiswa kwengxelo ye-QC kunye nedatha eluhlaza kwifomathi ye-fastq emva kokususa i-multiplexing kunye nokucoca i-Q30 reads.

Iingenelo

●Ukuguquguquka kweenkonzo zokulandelelanisa:umthengi angakhetha ukulandelana ngomgca okanye ubungakanani bedatha.

●Imveliso ephezulu yedatha:1500 Gb/umgca

●Ukuhanjiswa kwengxelo ye-QC yokulandelelana:ngemilinganiselo yomgangatho, ukuchaneka kwedatha kunye nokusebenza ngokubanzi kweprojekthi yokulandelelana.

●Inkqubo yokulandelelanisa abantu abadala:ngexesha elifutshane lokubuyela umva.

●Ulawulo Oluqinileyo Lomgangatho: Sisebenzisa iimfuno ze-QC ezingqongqo ukuqinisekisa ukunikezelwa kweziphumo ezisemgangathweni ophezulu rhoqo.

Iimfuno zeSampuli

	Isixa seDatha (X)	Uxinaniso (qPCR/nM)	Umqulu
Indlela Engaphelelanga	X ≤ 10 Gb	≥ 1nM	≥ 25 μl
	10 Gb < X ≤ 50 Gb	≥ 2 nM	≥ 25 μl
	50 Gb < X ≤ 100 Gb	≥ 3 nM	≥ 25 μl
	X > 100 Gb	≥ 4 nM
Indlela enye	NgokweNdlela nganye	≥ 1.5 nM / Ichibi leThala leeNcwadi	≥ 25 μl / Ichibi leThala leeNcwadi

Ukongeza kuxinzelelo kunye nobungakanani obupheleleyo, kufuneka iphethini efanelekileyo yokuphakama.

Qaphela: Ukulandelelana kweendlela zeelayibrari ezinazo iintlobo ngeentlobo kufuna iPhiX inyuswe ukuze kuqinisekiswe ukuba isiseko sisebenza kakuhle.

Sicebisa ukuba singenise iilayibrari ezidityaniswe kwangaphambili njengeesampuli. Ukuba ufuna i-BMKGENE ukuba yenze i-library pooling, nceda ujonge kwi

iimfuno zethala leencwadi zokulandelelana kweendlela ezingaphelelanga.

Ubungakanani beThala leeNcwadi (Imephu yeNcam)

Incopho ephambili kufuneka ibe phakathi kwe-300-450 bp.

Amathala eencwadi kufuneka abe ne-peak enye ephambili, kungabikho ungcoliseko lwe-adaptha kwaye kungabikho zi-primer dimers.

Ukuhamba komsebenzi wenkonzo

Ngaphambili: Ukusebenzisana kweChromatin okusekelwe kwiHi-C

Okulandelayo: I-BMKMANU S3000_Spatial Transcriptome

Ingxelo yeQC yeThala leeNcwadi

Ingxelo ngomgangatho wethala leencwadi inikwa ngaphambi kokuba kulandelelaniswe, kuhlolwe ubungakanani bethala leencwadi, kwaye kuqhekeke.

Ingxelo ye-QC elandelelanayo

Itheyibhile 1. Izibalo malunga nedatha yokulandelelana.

Isazisi seSampuli	I-BMKID	Ukufunda okungavuthwanga	Idatha eluhlaza (bp)	Ukufundwa okucocekileyo (%)	Q20(%)	Q30(%)	I-GC(%)
C_01	BMK_01	22,870,120	6,861,036,000	96.48	99.14	94.85	36.67
C_02	BMK_02	14,717,867	4,415,360,100	96.00	98.95	93.89	37.08