I-Eukaryotic mRNA Sequencing-NGS
Iimbonakalo
● Ulungiselelo lwethala leencwadi le-mRNA elinokuzikhethela ukwenza ukuba kufunyanwe idatha yolandelelwano lwestrand-specific
● Uhlalutyo olwahlukileyo lwe-BI lugubungela zonke iimfuno zabaxhasi bethu
Iingenelo
●UbuGcisa obuBanzi: Senze ngaphezulu kweesampulu ezingama-600,000, ezithatha iindidi ezahlukeneyo zeesampulu ezifana neenkcubeko zeeseli, izicubu kunye nolwelo lomzimba. Sizisa ubutyebi bobuchule kwiprojekthi nganye.
●Ulawulo loMgangatho olungqongqo: Sisebenzisa iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kulungiselelo lwesampulu ukuya kulungiselelo lwethala leencwadi, ulandelelwano kunye ne-bioinformatics. Ukubeka iliso kwethu ngononophelo kuqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.
●Isichasiselo esiBanzi: Sisebenzisa i-database ezininzi ukuze sichaze ngokusebenzayo i-Differentially Expressed Genes (DEGs) kwaye senze uhlalutyo oluhambelanayo lokutyebisa. Le ndlela ibanzi ibonelela ngengqiqo kwiinkqubo zeselula kunye neemolekyuli eziphantsi kwempendulo ye-transcriptome, eqinisekisa ukuba ufumana lonke ulwazi olunokwenzeka malunga nedatha yovavanyo lwakho.
●Inkxaso yePost-Sales: Siyayiqonda indlela okubaluleke ngayo ukubakho, yiyo loo nto ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi ngeenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno zeSampulu kunye nokuhanjiswa
| Ithala leencwadi | Isicwangciso sokulandelelana | Idatha iyacetyiswa | Ulawulo lwemeko |
| UPoly A utyebile | Illumina PE150 I-DNBSEQ-T7 | 6 -10 Gb | Q30≥85% |
IiMfuno zeSampulu:
IiNucleotides:
IiMfuno zeSampuli zeMisimum:
| Conc.(ng/μl) | Imali (μg) | Ubunyulu | Ingqibelelo | |
| Ithala leencwadi elisemgangathweni | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | Kwizityalo: RIN≥4.0; Kwizilwanyana: RIN≥4.5; 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
| Ithala leencwadi elingumkhombandlela | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | Kwizityalo: RIN≥4.0; Kwizilwanyana: RIN≥4.5; 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
| Izityalo | Isilwanyana | IiArthropods | Igazi elipheleleyo | Iiseli |
| Ingcambu, Isiqu okanye iPetal: 450 mg Igqabi okanye Imbewu: 300 mg Isiqhamo: 1.2 g | Intliziyo okanye Amathumbu: 300 mg Viscera okanye Brain: 240 mg Izihlunu: 450 mg Amathambo, Iinwele okanye Ulusu: 1g | Izinambuzane: 6g I-crustacea: 300 mg | ityhubhu e-1 | 106 iiseli |
Unikezelo lweSampuli eCetyisiweyo
Isikhongozeli: 2 ml centrifuge ityhubhu (Ifoyile yeTin ayikhuthazwa)
Isampuli yokuleyibhela: Iqela+liphindaphinda umz. A1, A2, A3; B1, B2, B3... ...
Ukuthunyelwa ngenqanawa:
1. Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa kwaye zingcwatywe kumkhenkce owomileyo.
2. Iityhubhu zetafile ze-RNAs: Iisampulu ze-RNA zingomiswa kwi-tube ye-RNA yokuzinzisa (umzekelo, i-RNAstable®) kwaye ihanjiswe kwiqondo lokushisa.
IiMfuno zeSampulu kunye nokuhanjiswa
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukukhutshwa kwe-RNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Bioinformatics
Kwiisampulu ezinereferensi yejenome ekhoyo, uhlalutyo lweBI lungolu lulandelayo:
ØUlawulo lomgangatho wedatha ekrwada
ØReference genome ulungelelwaniso
ØUhlalutyo lwesakhiwo esibhaliweyo
ØUbungakanani benkcazo
ØUhlalutyo lwentetho eyahlukileyo
ØInkcazo yomsebenzi kunye nokutyebisa
Kwiisampulu ezingenayo i-genome yesalathiso, olu lukhetho olukhoyo:
• Ulawulo lomgangatho wedatha ekrwada
•INdibano kunye nokulungelelaniswa kwesakhiwo
•Uhlalutyo lwentetho eyahlukileyo
•Inkcazo yomsebenzi kunye nokutyebisa
Reference Genome Ulungelelwaniso
Ukugcinwa kwedatha
Ulungelelwaniso lwesampulu kunye novavanyo lweereplicate zebhayoloji
Iimfuza ezivezwe ngokwahlukileyo (DEGs)
Inkcazo esebenzayo yee-DEGs
Uphuculo oluSebenzayo lweeDEGs
Phonononga inkqubela phambili eququzelelwa yi-BMKGene's eukaryotic NGS mRNA iinkonzo zolandelelwano ngengqokelela egciniweyo yopapasho.
Huang, L. et al. (2023) 'I-Triclosan kunye ne-triclocarban zenza buthathaka umthamo wokunuka kwe-goldfish ngokunyanzelela ukuqondwa kwevumba elimnandi, ukuphazamisa ukuhanjiswa komqondiso onukayo, kunye nokuphazamiseka kokusetyenzwa kolwazi olunukayo';Uphando lwamanzi, 233, iphe. 119736. doi: 10.1016/J.WATRES.2023.119736.
Jia, LJ et al. (2023) 'I-Aspergillus fumigatus iqweqwedisa i-p11 yomntu ukuze ibuyisele i-phagosomes equlethe umngundo kwindlela engonakalisi',Umamkeli weSeli kunye neMicrobe, 31(3), iphepha 373-388.e10. doi: 10.1016/J.CHOM.2023.02.002.
Jin, K. et al. (2022) 'Iizinto ze-TCP zokuBhala eziBandakanyiweyo kuPhuhliso lwe-Shoot ye-Ma Bamboo (Dendrocalamus latiflorus Munro)',Imida kwiNzululwazi yeZityalo, 13, iphe. 884443. doi: 10.3389/FPLS.2022.884443/BIBTEX.
Wen, X. et al. (2022) 'I-Chrysanthemum lavandulifolium genome kunye nendlela yemolekyuli ephantsi kweentlobo ezahlukeneyo ze-capitulum',Uphando lweHorticulture, 9. doi: 10.1093/HR/UHAB022.
UZhang, uYujie et al. (2023) 'I-cascade nanoreactor yokuphucula unyango lwe-sonodynamic kumhlaza we-colorectal nge-synergistic ROS augment kunye nokuvalwa kwe-autophagy',Nano Namhlanje, 49, iphe. 101798. doi: 10.1016/J.NANTOD.2023.101798.
