Ukulandelelana kwe-mRNA ye-Eukaryotic-NGS
Iimbonakalo
● Ukulungiselela ilayibrari ye-mRNA enokukhokela ukuze ukwazi ukufumana idatha yokulandelelana kwemigca ethile
● Uhlalutyo lwe-BI olwahlukileyo lugubungela zonke iimfuno zabathengi bethu
Iingenelo
●Ubuchule Obubanzi: Siye sacubungula iisampulu ezingaphezu kwama-600,000, sigubungela iintlobo ezahlukeneyo zesampulu ezifana neenkcubeko zeeseli, izicubu zomzimba, kunye nolwelo lomzimba. Sizisa ubutyebi bobuchule kuyo yonke iprojekthi.
●Ulawulo Oluqinileyo Lomgangatho: Sisebenzisa amanqaku olawulo oluphambili kuzo zonke izigaba, ukusuka ekulungiseleleni iisampulu ukuya ekulungiseleleni ithala leencwadi, ukulandelelana kunye ne-bioinformatics. Ukubeka iliso kwethu ngononophelo kuqinisekisa ukunikezelwa kweziphumo ezisemgangathweni ophezulu rhoqo.
●Ingcaciso ebanzi: Sisebenzisa ii-database ezininzi ukuze sichaze ngokusebenzayo ii-Differentially Expressed Genes (DEGs) kwaye senze uhlalutyo lokufumisa oluhambelanayo. Le ndlela ibanzi inika ulwazi kwiinkqubo zeseli kunye neemolekyuli eziphantsi kwempendulo ye-transcriptome, ukuqinisekisa ukuba ufumana lonke ulwazi olunokwenzeka malunga nedatha yovavanyo lwakho.
●Inkxaso Emva koThengiso: Siyaqonda ukubaluleka kokubakho, yiyo loo nto ukuzibophelela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye nexesha lenkonzo emva kokuthengiswa kweenyanga ezi-3. Ngeli xesha, sinikezela ngokulandela iprojekthi, uncedo lokusombulula iingxaki, kunye neeseshoni ze-Q&A ukuphendula nayiphi na imibuzo enxulumene neziphumo.
Iimfuno zeSampuli kunye nokuHanjiswa
| Ithala leeNcwadi | Icebo lokulandelelanisa izinto | Idatha iyacetyiswa | Ulawulo lwemeko |
| I-Poly A etyebileyo | I-Illumina PE150 I-DNBSEQ-T7 | 6 -10 Gb | Q30≥85% |
Iimfuno zeSampuli:
Iinukleotide:
Iimfuno zeSampuli eziNcinci:
| I-Conc.(ng/μl) | Ubungakanani (μg) | Ubunyulu | Ingqibelelo | |
| Ithala leeNcwadi eliQhelekileyo | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Ungcoliseko lweproteni okanye lwe-DNA oluncinci okanye alubonakali kwijeli. | Kwizityalo: I-RIN≥4.0; Kwizilwanyana: I-RIN≥4.5; 5.0≥28S/18S≥1.0; ukuphakama okulinganiselweyo okanye okungekho siseko |
| Ithala leeNcwadi eliLawulayo | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Ungcoliseko lweproteni okanye lwe-DNA oluncinci okanye alubonakali kwijeli. | Kwizityalo: I-RIN≥4.0; Kwizilwanyana: I-RIN≥4.5; 5.0≥28S/18S≥1.0; ukuphakama okulinganiselweyo okanye okungekho siseko |
| Izityalo | Isilwanyana | Ii-arthropod | Igazi elipheleleyo | Iiseli |
| Ingcambu, Isiqu okanye iPetal: 450 mg Igqabi okanye Imbewu: 300 mg Isiqhamo: 1.2 g | Intliziyo okanye Amathumbu: 300 mg I-Viscera okanye ubuchopho: 240 mg Izihlunu: 450 mg Amathambo, Iinwele okanye Ulusu: 1g | Izinambuzane: 6g I-Crustacea: 300 mg | Ityhubhu e-1 | Iiseli ezili-106 |
Ukuhanjiswa kweSampuli okuCetyisiweyo
Isikhongozeli: Ityhubhu ye-centrifuge ye-2 ml (Akukhuthazwa ukusebenzisa i-tin foil)
Ilebheli yesampulu: Iqela + ikopi umz. A1, A2, A3; B1, B2, B3... ...
Ukuthunyelwa:
1. Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa zize zingcwatywe kumkhenkce owomileyo.
2. Iityhubhu zetafile ze-RNA: Iisampulu ze-RNA zingomiswa kwityhubhu yokuzinzisa i-RNA (umz. i-RNAstable®) kwaye zithunyelwe kubushushu begumbi.
Iimfuno zeSampuli kunye nokuHanjiswa
Uyilo lovavanyo
Ukuhanjiswa kwesampulu
Ukukhupha i-RNA
Ukwakhiwa kweThala leeNcwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zasemva kokuthengisa
I-Bioinformatics
Kwiisampuli ezine-genome yesalathiso ekhoyo, uhlalutyo lwe-BI lulandelayo:
ØUlawulo lomgangatho wedatha eluhlaza
ØUlungelelwaniso lwe-genome yesalathiso
ØUhlalutyo lwesakhiwo semibhalo ebhaliweyo
ØUbungakanani bokuchaza
ØUhlalutyo lokubonakaliswa kokwahluka
ØIsichazi-magama somsebenzi kunye nokuphucula
Kwiisampuli ezingenayo i-genome ebhekisayo, olu khetho lufumanekayo:
• Ulawulo lomgangatho wedatha eluhlaza
•Ukudibanisa ngokulungiswa kwesakhiwo
•Uhlalutyo lokubonakaliswa kokwahluka
•Isichazi-magama somsebenzi kunye nokuphucula
Ulungelelwaniso lweeGenome ezibhekiselele kuzo
Ukwanda kwedatha
Ulwalamano lwesampulu kunye novavanyo lweekopi zebhayoloji
IiGene ezichazwe ngokwahlukileyo (ii-DEG)
Ingcaciso esebenzayo yee-DEG
Ukuphuculwa kokusebenza kwe-DEGs
Hlola inkqubela phambili eququzelelwe ziinkonzo ze-BMKGene zokulandelelana kwe-eukaryotic NGS mRNA ngokusebenzisa ingqokelela yeempapasho ezikhethiweyo.
UHuang, L. et al. (2023) 'I-Triclosan kunye ne-triclocarban zibuthathaka amandla okuhombisa e-goldfish ngokuthintela ukuqatshelwa kwevumba, ukuphazamisa ukudluliselwa kwesignali yokuhombisa, kunye nokuphazamisa ukucutshungulwa kolwazi lokuhombisa',Uphando lwaManzi, 233, iphe. 119736. doi: 10.1016/J.WATRES.2023.119736.
UJia, LJ et al. (2023) 'I-Aspergillus fumigatus ithatha i-p11 yomntu ukuze iqondise ii-phagosomes ezinefungi kwindlela engonakalisiyo',Umphathi weseli kunye neMicrobe, 31(3), iphepha 373-388.e10. doi: 10.1016/J.CHOM.2023.02.002.
UJin, K. et al. (2022) 'Izinto zeTCP Transcription ezibandakanyekayo ekuphuhlisweni kweZihluma zeMa Bamboo (Dendrocalamus latiflorus Munro)',Imida kwiSayensi yeZityalo, 13, iphe. 884443. doi: 10.3389/FPLS.2022.884443/BIBTEX.
UWen, X. et al. (2022) 'I-genome ye-Chrysanthemum lavandulifolium kunye nendlela esebenza ngayo i-molecular phantsi kweentlobo ezahlukeneyo ze-capitulum',Uphando lwezoLimo, 9. doi: 10.1093/HR/UHAB022.
UZhang, uYujie et al. (2023) 'I-cascade nanoreactor yokuphucula unyango lwe-sonodynamic kumhlaza we-colorectal ngokusebenzisa i-synergistic ROS augment kunye ne-autophagy blockage',I-Nano Namhlanje, 49, iphe. 101798. doi: 10.1016/J.NANTOD.2023.101798.
