Ngena kwi-BMKCloud

Iimveliso

I-Genetics yokuvela kwezinto

Umfanekiso obonisiweyo we-Evolutionary Genetics

I-Evolutionary Genetics yinkonzo ebanzi yokulandelelanisa eyenzelwe ukubonelela ngengcaciso ecacileyo yenguquko ngaphakathi kweqela elikhulu labantu, ngokusekelwe kwiinguquko zemfuza, kuquka ii-SNP, ii-InDels, ii-SV, kunye nee-CNV. Le nkonzo ibandakanya lonke uhlalutyo olubalulekileyo olufunekayo ukucacisa utshintsho lwenguquko kunye neempawu zemfuza zabemi, kuquka uvavanyo lwesakhiwo sabemi, ulwahluko lwemfuza, kunye nobudlelwane be-phylogenetic. Ngaphezu koko, ijonga kwizifundo ngokuhamba kwemfuza, okwenza kube lula ukuqikelela ubungakanani boluntu obusebenzayo kunye nexesha lokwahlukana. Izifundo zemfuza zenguquko zivelisa ulwazi oluxabisekileyo malunga nemvelaphi kunye nohlengahlengiso lweentlobo.

Apha kwi-BMKGENE, sinikezela ngeendlela ezimbini zokwenza izifundo ze-genetics eziziindalo kubantu abaninzi: ukusebenzisa i-whole-genome sequencing (WGS) okanye ukukhetha indlela ye-reduced representation genome sequencing method, i-Specific-Locus Amplified Fragment (SLAF) ephuhliswe ngaphakathi endlwini. Ngelixa i-WGS ifanele ii-genomes ezincinci, i-SLAF ivela njengendlela engabizi kakhulu yokufunda abantu abakhulu abanee-genomes ezinde, inciphisa ngokufanelekileyo iindleko zokulandelelana.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

I-Bioinformatics

Iziphumo zeDemo

Iincwadi ezibalaseleyo

Iingenelo zeNkonzo

UTakagi nabanye.,Ijenali yezityalo, 2013

●Uhlalutyo olupheleleyo lwe-bioinformatics:ukuvumela ukuqikelela ukwahluka kwezakhi zofuzo, okubonisa amandla okuzivelela kweentlobo ngeentlobo, kunye nokuveza ubudlelwane obuthembekileyo be-phylogenetic phakathi kweentlobo ngeentlobo ngempembelelo encinci yokuzivelela okudibeneyo kunye nokuzivelela okuhambelanayo

●Uhlalutyo olukhethiweyo olukhethiweyo: njengoqikelelo lwexesha lokwahlukana kunye nesantya ngokusekelwe kutshintsho kwinqanaba le-nucleotide kunye ne-amino acids.

●Ubungcali obuninzi kunye neerekhodi zokupapasha: I-BMKGene iqokelele amava amaninzi kwiiprojekthi zemfuza zabantu kunye ne-evolutionary iminyaka engaphezu kwe-15, igubungela amawaka eentlobo zezilwanyana, njl. kwaye inegalelo kwiiprojekthi ezingaphezulu kwe-1000 ezikumgangatho ophezulu ezipapashwe kwi-Nature Communications, Molecular Plants, Plant Biotechnology Journal, njl.

● Iqela le-bioinformatics elinobuchule obuphezulu kunye nomjikelo omfutshane wohlalutyo: ngamava amahle kakhulu kuhlalutyo oluphambili lwe-genomics, iqela le-BMKGene linikezela ngohlalutyo olupheleleyo ngexesha elikhawulezayo lokutshintsha.

● Inkxaso Emva kokuthengisa:Ukuzibophelela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye nexesha leenkonzo emva kokuthengiswa kweenyanga ezi-3. Ngeli xesha, sinikezela ngokulandela iprojekthi, uncedo lokusombulula iingxaki, kunye neeseshoni ze-Q&A ukuphendula nayiphi na imibuzo enxulumene neziphumo.

Iinkcukacha zeNkonzo kunye neemfuno

Uhlobo lokulandelelana

Isikali sabantu abacetyiswayo

Icebo lokulandelelanisa izinto

Iimfuno zeNucleotide

Ulandelelwano lweGenome olupheleleyo

≥ Abantu abangama-30, kunye nabantu abali-10 kwiqela ngalinye

10x

Uxinzelelo: ≥ 1 ng/ µL

Isixa sisonke ≥ 30ng

Ukonakala okanye ukungcola okulinganiselweyo okanye okungekhoyo

Iqhekeza Elicacileyo Eliphakanyisiweyo (i-SLAF)

Ubunzulu bethegi:

10x

Inani leethegi:

<400 Mb: Kucetyiswa i-WGS

<1Gb: Iithegi ze-100K

1Gb

>2Gb: Iithegi ezingama-300K

Iithegi eziphezulu ze-500k

Uxinzelelo ≥ 5 ng/µL

Isixa-mali sisonke ≥ 80 ng

I-Nanodrop OD260/280=1.6-2.5

Ijeli ye-Agarose: akukho okanye akukho monakalo okanye ungcoliseko olulinganiselweyo

Ukuhamba komsebenzi wenkonzo

Uyilo lovavanyo

Ukuhanjiswa kwesampulu

Ukwakhiwa kweThala leeNcwadi

Ukulandelelana

Uhlalutyo lwedatha

Iinkonzo zasemva kokuthengisa

Ngaphambili: Uhlalutyo lweBulked Segregant

Okulandelayo: I-Metabolomics

Inkonzo ibandakanya uhlalutyo lwesakhiwo sabemi (umthi we-phylogenetic, i-PCA, itshathi yokwahlulwahlulwa kwabemi), ukwahlukana kwabemi, kunye nokukhethwa kwabemi (ukungalingani konxibelelwano, ukukhethwa kweendawo eziluncedo ngokukhetha). Inkonzo ingabandakanya nohlalutyo olulungiselelwe wena (umz. ixesha lokwahlukana, ukuhamba kwezakhi zofuzo).

*Iziphumo zedemo eziboniswe apha zonke zivela kwii-genomes ezipapashwe nge-BMKGENE

1. Uhlalutyo lwendaleko luqulathe ulwakhiwo lomthi we-phylogenetic, ulwakhiwo lwabemi kunye ne-PCA ngokusekelwe kutshintsho lwemfuza.

Umthi we-phylogenetic umele ubudlelwane be-taxonomic kunye nophuhliso phakathi kweentlobo ezinomzukulwana ofanayo.
I-PCA ijonge ukuqikelela ukusondelana phakathi kwabantu abangaphantsi.
Ulwakhiwo lwabemi lubonisa ukuba kukho inani elincinci labantu abahlukeneyo ngokwezakhi zofuzo ngokwee-allele frequency.

3-1 Umthi wePhylogenetic 3-2PCA 3-3Ulwakhiwo lwabemi

UChen, nabanye,I-PNAS, 2020

2. Ukutshiza okukhethayo

Ukucoca ngokukhetha kubhekisa kwinkqubo apho kukhethwa khona indawo enenzuzo kwaye amaxesha eendawo ezingenanto adibeneyo ayanda kwaye ezo zeendawo ezingadibaniyo ziyancipha, nto leyo ebangela ukuncipha kweendawo.

Ukufunyanwa kwe-genome ngokubanzi kwiindawo ezikhethiweyo zokuntywila kwenziwa ngokubala i-population genetic index (π，Fst, Tajima's D) yazo zonke ii-SNP ngaphakathi kwefestile etyibilikayo (100 Kb) kwinqanaba elithile (10 Kb).

Ukwahlukahlukana kweNucleotide(π）
4Ukwahluka kweNucleotide(π）

UD kaTajima
5Tajima's-D

Isalathisi sokulungiswa (Fst)

Isalathisi se-6Fixation (Fst)

UWu, nabanye.,Isityalo seMolekyuli, 2018

3. Ukuhamba kweGene

7Ukuhamba kwezakhi zofuzo

UWu, nabanye.,Isityalo seMolekyuli, 2018

4. Imbali yabantu

8Imbali yabantu

UZhang, nabanye.,I-Ecology yeNdalo kunye nokuZivela kwendalo, 2021

5. Ixesha lokwahlukana

9Ixesha lokwahlukana

UZhang, nabanye.,I-Ecology yeNdalo kunye nokuZivela kwendalo, 2021

Hlola inkqubela phambili eququzelelwe ziinkonzo ze-BMKGene ze-genetics eziguqukayo ngokusebenzisa ingqokelela yeempapasho ezikhethiweyo:

Hassanyar, AK et al. (2023) 'Ukufunyanwa kweeSNP Molecular Markers kunye neeCandidate Genes ezidityaniswe nokuxhathisa iintsholongwane zeSacbrood kwi-Apis cerana cerana Larvae yi-Whole-Genome Resequencing',Ijenali yamazwe ngamazwe yesayensi yeemolekyuli, 24(7). doi: 10.3390/IJMS24076238.

UChai, J. et al. (2022) 'Ukufunyanwa kwesalamander enkulu yaseTshayina, ecocekileyo ngokwemfuza, kudala amathuba amatsha olondolozo',Uphando lweZoology, 2022, uMqulu 43, Inqaku 3, Amaphepha: 469-480, 43(3), iphe. 469–480. doi: 10.24272/J.ISSN.2095-8137.2022.101.

UHan, M. et al. (2022) 'Iphethini yePhylogeographical kunye neMbali yoBume beNdalo ka-Elymus sibiricus L. kwiQinghai-Tibetan Plateau',Imida kwiSayensi yeZityalo, 13, iphe. 882601. doi: 10.3389/FPLS.2022.882601/BIBTEX.

UWang, J. et al. (2022) 'Iingcamango ze-genomic malunga nokuguquka kwe-longan ukusuka kwi-gene assembly ye-chromosome-level genome kunye ne-population genomics ye-longan accessions',Uphando lwezoLimo, 9. doi: 10.1093/HR/UHAC021.