Ngena kwi-BMKCloud

Iimveliso

I-Exosomal mRNA/LncRNA/CirrcRNA Sequencing-Illumina

Umfanekiso oBonisiweyo we-mRNA/LncRNA/CirrcRNA Sequencing-Illumina

Ii-Exosomes zii-vesicles ezincinci ezikhutshwa ziiseli, zihlala zinobubanzi obuphakathi kwee-nanometers ezingama-30 ukuya kwi-100. Ezi vesicles ziqulathe ii-RNA ezahlukeneyo. Ii-Exosomes zikholelwa ukuba zidlala indima ebalulekileyo kunxibelelwano phakathi kweeseli, iimpendulo zomzimba, kunye nophuhliso lwezifo, kwaye zinokusasazwa kwezinye iindawo zomzimba ngolwelo lomzimba olufana ne-plasma, amathe, kunye nomchamo. Ziphethe ii-biomolecules ezithile ukulawula imisebenzi yeeseli ezamkelayo, zichaphazela imeko ye-physiological yeseli. Ii-Exosomes zikwathathwa njengezidlala indima ebalulekileyo ekuphuhlisweni kwesifo, kubandakanya umhlaza, izifo ze-neurodegenerative, kunye neemeko zokudumba. Uphando ngee-exosomes lubonelela ngengqiqo entsha kunye neendlela zokuxilonga, unyango, kunye nokuthintela izifo.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

I-Bioinformatics

Iziphumo zeDemo

Iimbonakalo

● Iiprobe ezikhethekileyo zisetyenziselwa ukuphelisa i-ribosomal cDNA.

● Ukwakhiwa kwethala leencwadi elikhethekileyo.

● Umsebenzi omnye wokufumana idatha ye-mRNA, i-lncRNA kunye ne-circRNA ngaxeshanye.

Iingenelo zeNkonzo

● Ulawulo Oluqinileyo Lomgangatho: Sisebenzisa amanqaku olawulo oluphambili kuzo zonke izigaba, ukusuka ekulungiseleleni iisampulu kunye nethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics. Olu hlolo olucokisekileyo luqinisekisa ukunikezelwa kweziphumo ezisemgangathweni ophezulu rhoqo.

● Inkxaso Emva Kokuthengisa: Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye nexesha leenkonzo emva kokuthengisa leenyanga ezi-3. Ngeli xesha, sinikezela ngokulandela iprojekthi, uncedo lokusombulula iingxaki, kunye neeseshoni ze-Q&A ukuphendula nayiphi na imibuzo enxulumene neziphumo.

Iimfuno zeSampuli kunye nokuHanjiswa

Ithala leeNcwadi	Iqonga	Idatha ecetyiswayo	Idatha ye-QC
ilayibrari yesikhokelo ephelileyo ye-rRNA	I-Illumina PE150	10-16 Gb	Q30≥85%

Iimfuno zeSampuli

Iinukleotide:

Isixa (ng)	Umthamo (μL)
40	20

Izicubu:

I-Exosome: 100 μL; 40 μg; >10^9 amasuntswana/mL
I-serum/plasma: 5 mL
I-supernatant yenkcubeko yeseli: 100 mL
Umchamo: 100 mL
Amathe: 15 mL
Ukukhupha iimpumlo: 15 mL
Ulwelo lweCerebrospinal: 15 mL
Ulwelo lwe-Amniotic: 50 mL
Amadlozi: 5 mL
Inyongo: 5 mL
Ii-Ascites: 50 mL
Ubisi: 15 mL
Ulwelo lweFollicular: 15 mL
I-supernatant yelindle: 50 mL
I-supernatant yenkcubeko ye-microbial: 100 mL
Izicubu zezilwanyana: 3 g
Izicubu zesityalo: 1 g

Ukuhanjiswa kweSampuli okuCetyisiweyo

Isikhongozeli: ityhubhu ye-centrifuge ye-2 mL (Akukhuthazwa ukusebenzisa i-tin foil)
Iilebheli zesampulu: Iqela + ikopi umz. A1, A2, A3; B1, B2, B3.
Ukuthunyelwa:
1. Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa zize zingcwatywe kumkhenkce owomileyo.
2. Iityhubhu ze-RNAstable: Iisampulu ze-RNA zingomiswa kwityhubhu yokuzinzisa i-RNA (umz. i-RNAstable®) kwaye zithunyelwe kubushushu begumbi.