Ngena kwi-BMKCloud

Iimveliso

I-TGuide Smart Magnetic Tissue DNA Kit

Umfanekiso oBonisiweyo we-TGuide Smart Magnetic Tissue DNA Kit

Ikhithi ye-cartridge/plate reagent ezalisiweyo kwangaphambili yokukhupha i-genomic DNA kwizicubu zezilwanyana

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Inombolo yekhathalogu / ukupakisha

Hayi ikati	ID	Inani lamalungiselelo
4995038(ipleyiti)	DP602-E	96
4993547 (ikhatriji)	I-DP602-DE	48

Isikhokelo sokukhetha i-reagent ye-TGuide S16

I-Nuclei Acid	Uhlobo lweSampuli	Igama lemveliso	Ubungakanani bokupakisha (ukulungiselela) Ikhatriji/ ipleyiti	Inombolo yekati. Ikhatriji/ipleyiti
i-DNA	Izicubu zezilwanyana	I-TGuide Smart Magnetic Tissue DNA Kit	48/96	4993547/4995038
i-DNA	Isityalo kunye neMbewu	I-TGuide Smart Magnetic Plant DNA Kit	48	4993548
i-DNA	Umhlaba/Isitulo	I-TGuide Smart Soil/Stool DNA Kit	48	4993549
i-DNA	Iimveliso zeGel kunye nePCR	Ikhithi yokuCoca i-DNA ehlakaniphileyo ye-TGuide	48	4993550
i-DNA	Igazi Elipheleleyo	I-TGuide Smart Blood Genomic DNA Kit	48/96	4993703/4995206
i-DNA	Amabala eSeli/eSwab/omileyo, njl.	I-TGuide Smart Universal DNA Kit	48/96	4993704/4995040
I-RNA	Igazi/Iseli/Izicubu	I-TGuide Smart Blood/Cell/Tissue RNA Kit	48/96	4993551/4995043
I-RNA	Isityalo kunye neMbewu	I-TGuide Smart Magnetic Plant RNA Kit	48	4993552
I-DNA/RNA	Ii-Fluids ezingenamaSeli (i-Serum, njl.njl.)	I-TGuide Smart Viral DNA/RNA Kit	48/96	4993702/4995207

Intshayelelo yemveliso

Coca i-DNA kwi-tissue ye-10-50 mg ngaphantsi kwemizuzu engama-50.

I-DNA ecociweyo ngale khithi iyasebenza kwiindlela ezahlukeneyo zokusetyenziswa, kubandakanya ukugaya, i-PCR, ukwakhiwa kwethala leencwadi, i-Southern hybridization, kunye nezinye iimvavanyo.

Umthamo we-flexible elution ukuvumela ukuba ufumane uxinaniso olukhulu okanye isivuno esikhulu.

Iimbonakalo

Kulula kakhulu ukuyisebenzisa

Akukho msebenzi wongezelelweyo wokufaka imibhobho.

Umthamo we-elution ungalungiswa njengoko kufuneka.

Ukufakela nokusebenza kufuna uqeqesho oluncinci. Ngeenkqubo ezifakwe kwangaphambili, khupha ikhatriji, khetha iprotocol kwaye usebenzise uvavanyo lwakho. Iinkqubo zilungele ukusetyenziswa, kwaye ziyakwazi ukuhlengahlengiswa.

Ii-reagents ezilayishwe kwangaphambili kunye nezinto ezisetyenziswayo ezilahlwayo ezifanayo.

Ukuphathwa kweekhemikhali kuyasuswa kakhulu ukuqinisekisa ukhuseleko lokusebenza.

Isiphumo seDemo

Ukukhupha iDNA yezilwanyana

Isiphumo sovavanyo: i-DNA ye-genomic yezicubu zezilwanyana ekhutshwa ngokuzenzekelayo yi-TGuide S16 exhotyiswe nge-reagent TGuide Smart Magnetic Tissue DNA Kit (4993547) inemveliso ephezulu kunye nobumsulwa, okulingana ne-DNA ekhutshwe yi-spin column-based extraction protocol. Ubumsulwa buyafana, nge-OD260/OD280 malunga ne-1.7~1.9 kunye ne-OD260/OD230 > 1.7. Ukuqukumbela, i-TGuide S16 ingasetyenziselwa ukukhupha i-genomic DNA kwizicubu zezilwanyana endaweni yesisombululo sokukhupha esisekelwe kwi-spin-column.

Umthamo wokulayisha: 2 μl.

Uxinzelelo lwejeli ye-Agarose: 1%. I-Electrophoresis imizuzu engama-20 kwi-6 V/cm;

M:TIANGEN Uphawu lwesithathu

1-4: intliziyo 5-8: i-spleen 9-12: imiphunga 13-16: izintso 17-20: ubuchopho

Iisampuli ezimbini zokuqala zethishu nganye zikhutshwe yikhithi yokukhupha esekwe kwi-spin-column, kwaye ezimbini zokugqibela zikhutshwe ngokuzenzekelayo yi-TGuide S16.

Umthombo wesampulu: iimpuku ze-CD-1

Ngaphambili: I-TGuide Smart Universal DNA Kit

Okulandelayo: I-TGuide Smart Blood Genomic DNA Kit