Iimveliso

I-Fungal Genome

I-Biomarker Technologies ibonelela ngesaveyi ye-genome, i-genome entle kunye ne-pene-complete genome ye-fungal ngokuxhomekeke kwinjongo ethile yophando.Ukulandelelana kweGenome, indibano kunye nenkcazo esebenzayo inokufezekiswa ngokudibanisa isizukulwana esilandelayo + Ukulandelelana kwesizukulwana sesithathu ukuphumeza indibano yegenome yenqanaba eliphezulu.Itekhnoloji ye-Hi-C inokuqeshwa ukuququzelela ukudibanisa i-genome kwinqanaba le-chromosome.

Iqonga:I-PacBio Sequel II

Nanopore PromethION P48

I-Illumina NovaSeq Platform

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Iziphumo zeDemo

Iinkonzo eziluncedo

● Izicwangciso ezininzi zolandelelwano ezikhoyo kwiinjongo zophando ezahlukeneyo

● Unamava kakhulu ekudityanisweni kwegenome yefungi ene-pene-complete genome engaphezu kwe-10,000 edityanisiweyo.

● Iqela lenkxaso yobugcisa emva kokuthengiswa lizalisekisa iimfuno ezithe ngqo zophando.

IiNgcaciso zeNkonzo

Inkonzo	Isicwangciso solandelelwano	Umgangatho uqinisekisiwe	Ixesha eliqikelelweyo lokujika
Imephu entle yokungunda	Illumina 50X+Nanopore 100X	Contig N50≥2 Mb	Iintsuku ezingama-35 zokusebenza
Imephu entle yokungunda	I-PacBio HiFi 30X	Contig N50≥2 Mb	Iintsuku ezingama-35 zokusebenza
Imaphu yokungunda ipene-gqibezela	Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	I-Chromosome anchoring ratio >90%	Iintsuku ezingama-45 zokusebenza

Uhlalutyo lwe-Bioinformatics

● Ulawulo lomgangatho wedatha ekrwada

● Indibano yeGenome

● Uhlalutyo lwecandelo leGenome

● Inkcazo yomsebenzi wemfuza

● Uhlalutyo lokuthelekisa i-genomic

IiMfuno zeSampulu kunye nokuhanjiswa

IiMfuno zeSampulu:

KubaIzicatshulwa zeDNA:

Uhlobo lweSampuli	Isixa	Ukugxininisa	Ubunyulu
Izicatshulwa zeDNA	＞ 1.2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Ngeisampulu zethishu:

Uhlobo lwesampuli

Unyango olucetyiswayo lwesampulu

Ukugcinwa kwesampuli kunye nokuthunyelwa

Unicellular fungus

Jonga igwele phantsi kwemicroscope kwaye uziqokelele kwisigaba sazo sokubonisa

Dlulisa inkcubeko (equlethe malunga neeseli ze-3-4.5e9) kwi-1.5 okanye i-2 ml eppendorf.(Gcina emkhenkceni)

I-Centrifuge ityhubhu ye-1 min kwi-14000 g ukuqokelela ibhaktheriya kunye nokususa i-supernatant ngononophelo.

Vala ityhubhu kwaye ukhenkceze iibhaktheriya kwi-nitrogen engamanzi malunga ne-1-3 h.Gcina ityhubhu kwi -80 ℃ efrijini.

Umkhenkce iisampulu kulwelo nitrogen iiyure 3-4 kwaye ugcine kulwelo nitrogen okanye -80 degree ukuya ugcino ixesha elide.Isampulu yokuthumela ngenqanawe eyomileyo iyafuneka.

Ifungus ezinkulu

Kunconywa izicubu kwisigaba sokukhula.

Hlanza izicubu ngamanzi angenayo i-endotoxin, emva koko i-70% ye-ethonal.

Gcina isampuli kwi-cryo-tubes.