Uhlalutyo loMbutho weGenome-wide
Ukuhamba komsebenzi
Iingenelo zeNkonzo
●Ubungcali obuninzi kunye neerekhodi zokupapasha: ngamava aqokelelweyo kwiGWAS, iBMKGene igqibe amakhulu eeprojekthi zeentlobo ngeentlobo kuphando lwe-GWAS lwabemi, yanceda abaphandi ukuba bapapashe amanqaku angaphezu kwe-100, kwaye impembelelo eqokelelweyo ifikelele kuma-500.
● Uhlalutyo olupheleleyo lwe-bioinformatics: indlela yokusebenza ibandakanya uhlalutyo lonxulumano lweempawu ze-SNP, olunikezela ngeseti yeejini ezikhethiweyo kunye nesichazi-magama esisebenzayo esihambelanayo.
●Iqela le-bioinformatics elinobuchule obuphezulu kunye nomjikelo omfutshane wohlalutyo: ngamava amahle kakhulu kuhlalutyo oluphambili lwe-genomics, iqela le-BMKGene linikezela ngohlalutyo olupheleleyo ngexesha elikhawulezayo lokutshintsha.
●Inkxaso emva kokuthengisa:Ukuzibophelela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye nexesha leenkonzo emva kokuthengiswa kweenyanga ezi-3. Ngeli xesha, sinikezela ngokulandela iprojekthi, uncedo lokusombulula iingxaki, kunye neeseshoni ze-Q&A ukuphendula nayiphi na imibuzo enxulumene neziphumo.
Iinkcukacha kunye neemfuno zenkonzo
| Uhlobo lokulandelelana | Isikali sabantu abacetyiswayo | Icebo lokulandelelanisa izinto | Iimfuno zeNucleotide |
| Ulandelelwano lweGenome olupheleleyo | Iisampulu ezingama-200 | 10x | Uxinzelelo: ≥ 1 ng/ µL Isixa sisonke ≥ 30ng Ukonakala okanye ukungcola okulinganiselweyo okanye okungekhoyo |
| Iqhekeza Elicacileyo Eliphakanyisiweyo (i-SLAF) | Ubunzulu bethegi: 10x Inani leethegi: < 400 Mb: Kucetyiswa i-WGS < 1Gb: Iithegi ze-100K 1Gb > 2Gb: Iithegi ezingama-300K Iithegi eziphezulu ze-500k | Uxinzelelo ≥ 5 ng/µL Isixa-mali sisonke ≥ 80 ng I-Nanodrop OD260/280=1.6-2.5 Ijeli ye-Agarose: akukho okanye akukho monakalo okanye ungcoliseko olulinganiselweyo
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Ukukhetha Izinto
Iintlobo ezahlukeneyo, iintlobo ezincinci, iindawo zokuhlala/ii-genebanks/iintsapho ezixubileyo/izixhobo zasendle
Iintlobo ezahlukeneyo, iintlobo ezincinci, iindawo zokuhlala
Usapho lomzali omnye/usapho lomzali omnye/izixhobo zasendle
Ukuhamba komsebenzi wenkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampulu
Ukukhutshwa kwe-RNA
Ukwakhiwa kweThala leeNcwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zasemva kokuthengisa
Kubandakanya uhlalutyo olulandelayo:
- Uhlalutyo lonxulumano lwe-genome ngokubanzi: I-LM, i-LMM, i-EMMAX, imodeli ye-FASTLMM
- Isichazi-magama esisebenzayo se-genes ezikhethiweyo
Uhlalutyo lonxulumano lweempawu ze-SNP – iploti yaseManhattan
Uhlalutyo lonxulumano lweempawu ze-SNP – iploti ye-QQ
Hlola inkqubela phambili eququzelelwa ziinkonzo ze-BMKGene's de GWAS ngokusebenzisa ingqokelela yeempapasho ezikhethiweyo:
Lv, L. et al. (2023) 'Ukuqonda isiseko semfuza sokunyamezelana kwe-ammonia kwi-razor clam Sinonovacula constricta ngophando lobudlelwane be-genome',Ukulima iintlanzi zasemanzini, 569, iphe. 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
ULi, X. et al. (2022) 'Uhlalutyo lwe-Multi-omics lwee-398 foxtail millet accessions lubonisa iindawo ze-genomic ezinxulumene nokufakwa ekhaya, iimpawu ze-metabolite, kunye neziphumo zokulwa nokuvuvukala',Isityalo seMolekyuli, 15(8), iphepha 1367–1383. doi: 10.1016/j.molp.2022.07.003.
ULi, J. et al. (2022) 'Imephu yoManyano lweGenome-Wide yeHullless Barely Phenotypes kwiNdawo yeMbalela',Imida kwiSayensi yeZityalo, 13, iphe. 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. et al. (2021) 'I-GmST1, ebhala i-sulfotransferase, inika ukumelana neentlobo ze-soybean mosaic virus G2 kunye ne-G3',Isityalo, iSeli kunye neNdalo, 44(8), iphepha 2777-2792. doi: 10.1111/PCE.14066.
