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Ulandelelwano lweGenome

  • Plant/Animal De novo Genome Sequencing

    Isityalo / isilwanyana De novo Genome Sequencing

    De novoulandelelwano lubhekisa kulwakhiwo lwegenome yohlobo lonke kusetyenziswa ubugcisa bokulandelelana, umz. iPacBio, iNanopore, iNGS, njl., ngokungabikho kwegenome yereferensi.Uphuculo oluphawulekayo kubude bokufunda besizukulwana sesithathu sobuchwephesha bokulandelelana buye bazisa amathuba amatsha ekuhlanganiseni i-genomes enzima, efana ne-heterozygosity ephezulu, umlinganiselo ophezulu wemimandla ephindaphindiweyo, i-polyploids, njl. ukusombulula izinto eziphindaphindwayo, imimandla enomxholo we-GC ongaqhelekanga kunye neminye imimandla entsonkothileyo kakhulu.

    Iqonga: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq6000

  • Hi-C based Genome Assembly

    INdibano yeGenome esekwe kwi-Hi-C

    I-Hi-C yindlela eyilelwe ukubamba ulungelelwaniso lwechromosome ngokudibanisa ukudibana okusekwe ngokusondeleyo kunye nolandelelwano oluphezulu.Ukuqina kolu nxibelelwano kukholelwa ukuba lunxulunyaniswa kakubi nomgama womzimba kwiichromosomes.Ke ngoko, idatha ye-Hi-C inokukhokela ukudityaniswa, ukulandelelana kunye nokuqhelaniswa kolandelelwano oludityanisiweyo kwi-genome eyidrafti kunye nokubethelela ezo kwinani elithile lekhromozomi.Obu buchwepheshe buxhobisa indibano yegenome yenqanaba lechromosome ngokungabikho kwemephu yemfuzo esekwe ngabantu.Yonke i-genome enye idinga i-Hi-C.

    Iqonga: Illumina NovaSeq6000 / DNBSEQ

  • Evolutionary Genetics

    I-Evolutionary Genetics

    I-Evolutionary genetics yinkonzo yolandelelwano epakishweyo eyenzelwe ukunika utoliko olubanzi kulwazi lwendaleko lwezixhobo ezinikezelweyo ezisekelwe kuguquko lwemfuzo, kubandakanywa ii-SNP, ii-Indels, ii-SV kunye nee-CNV.Ibonelela ngalo lonke uhlalutyo olusisiseko olufunekayo ekuchazeni utshintsho lwendaleko kunye neempawu zemfuzo zabantu, ezifana nokwakheka kwabantu, iyantlukwano yemfuza, ubudlelwane be-phylogeny, njl.

  • Comparative Genomics

    Ukuthelekisa iGenomics

    Ukuthelekisa i-genomics ngokoqobo kuthetha ukuthelekisa ulandelelwano olupheleleyo lwe-genome kunye nezakhiwo zeentlobo ezahlukeneyo.Olu qeqesho lujolise ekubonakaliseni ukuvela kweentlobo, umsebenzi wemfuza, indlela yokulawula imfuza kwinqanaba le-genome ngokuchonga izakhiwo zolandelelwano kunye nezinto ezigciniweyo okanye ezahlulayo kwiintlobo ezahlukeneyo zeentlobo.Uphononongo oluqhelekileyo lothelekiso lwe-genomics lubandakanya ucazululo kusapho lwemfuza, uphuhliso lwendaleko, uphindaphindo lwegenome epheleleyo, uxinzelelo olukhethiweyo, njl.njl.

  • Bulked Segregant analysis

    Uhlalutyo lwe-Bulked Segregant

    Uhlalutyo lwe-bulked segregant (BSA) bubuchule obusetyenziselwa ukuchonga ngokukhawuleza i-phenotype ehambelana neempawu zofuzo.Ukuhamba komsebenzi okuphambili kwe-BSA kuqulethe ukukhetha amaqela amabini abantu abaneephenotypes ezichasene kakhulu, ukudibanisa i-DNA yabo bonke abantu ukwenza isambuku se-DNA, ukuchonga ulandelelwano olwahlukileyo phakathi kwamachibi amabini.Obu buchule busetyenziswe kakhulu ekuchongeni iimpawu zofuzo ezinxulunyaniswa ngamandla nemfuza ekujoliswe kuyo kwiigenome zezityalo/zezilwanyana.

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