Ngena kwi-BMKCloud

Iimveliso

Ukusebenzisana kweChromatin okusekelwe kwiHi-C

Umfanekiso oPhambili woNxibelelwano lweChromatin olusekelwe kwiHi-C

I-Hi-C yindlela eyilelwe ukubamba ulwakhiwo lwe-genomic ngokudibanisa unxibelelwano olusekwe ekujongeni ukusondela kunye nokulandelelana okuphezulu. Le ndlela isekelwe kwi-chromatin crosslinking kunye ne-formaldehyde, ilandelwe kukugaya kunye nokudibanisa kwakhona ngendlela yokuba kuphela iziqwenga ezidityaniswe ngokudibeneyo eziya kwenza iimveliso zokudibanisa. Ngokulandelanisa ezi mveliso zokudibanisa, kunokwenzeka ukufunda ulungelelwaniso lwe-3D lwe-genome. I-Hi-C ivumela ukufunda ukusasazwa kweendawo ze-genome ezipakishwe kancinci (ii-compartments ze-A, i-euchromatin) kwaye kunokwenzeka ukuba zisebenze ngokubhala, kunye neendawo ezipakishwe kakhulu (ii-compartments ze-B, i-Heterochromatin). I-Hi-C ingasetyenziselwa ukukhomba ii-Topologically Associated Domains (ii-TAD), iindawo ze-genome ezinezakhiwo ezigobileyo kwaye kusenokwenzeka ukuba zineendlela ezifanayo zokubonakalisa, kunye nokuchonga ii-chromatin loops, iindawo ze-DNA ezinamathele kunye ziiproteni kwaye zihlala zityebile kwizinto ezilawulayo. Inkonzo yokulandelelana kwe-BMKGene ye-Hi-C inika abaphandi amandla okuphonononga ubungakanani bendawo ye-genomics, ivula iindlela ezintsha zokuqonda ukulawulwa kwe-genome kunye nefuthe layo kwimpilo nakwizifo.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

I-Bioinformatics

Iziphumo zedemo

Iincwadi ezibalaseleyo

Iimpawu zeNkonzo

● Ukulandelelana kwe-Illumina NovaSeq kunye ne-PE150.

● Inkonzo ifuna iisampulu zezicubu, endaweni yee-nucleic acids ezikhutshiweyo, ukuze zidibane ne-formaldehyde kwaye zigcine ukusebenzisana kwe-DNA-protein.

● Uvavanyo lwe-Hi-C lubandakanya ukuthintelwa kunye nokulungiswa kwesiphelo seencam ezinamathelayo nge-biotin, kulandele ukujikeleza kweencam ezibuthuntu eziphumayo ngelixa kugcinwa ukusebenzisana. Emva koko i-DNA itsalwa phantsi ngee-streptavidin beads kwaye icocwe ukuze kulungiselelwe ithala leencwadi kamva.

Iingenelo zeNkonzo

●Uyilo lwe-Enzyme yoThintelo oluLungileyo: ukuqinisekisa ukusebenza kakuhle kwe-Hi-C kwiintlobo ezahlukeneyo ngezibini zokusebenzisana ezisebenza ukuya kuthi ga kwi-93%.

●Iingxelo zoBugcisa obuBanzi kunye nokuPapasha:I-BMKGene inamava amaninzi kwiiprojekthi zokulandelelana ze-Hi-C ezingaphezu kwama-2000 ezivela kwiintlobo ezahlukeneyo ezingama-800 kunye neepatenti ezahlukeneyo. Amatyala apapashiweyo angaphezu kwe-100 ane-accumulative impact factor engaphezulu kwama-900.

●Iqela le-Bioinformatics elinobuchule obuphezulu:kunye namalungelo okushicilela angaphakathi kunye namalungelo okushicilela esoftware kwiimvavanyo ze-Hi-C kunye nohlalutyo lwedatha kunye nesoftware yedatha yokubona ebonakalayo ephuhlisiweyo.

●Inkxaso emva kokuthengisa:Ukuzibophelela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye nexesha leenkonzo emva kokuthengiswa kweenyanga ezi-3. Ngeli xesha, sinikezela ngokulandela iprojekthi, uncedo lokusombulula iingxaki, kunye neeseshoni ze-Q&A ukuphendula nayiphi na imibuzo enxulumene neziphumo.

●Ingcaciso ebanzi: sisebenzisa iidathabheyisi ezininzi ukuze sichaze iijini ngendlela esebenzayo ngeenguqu ezichongiweyo kwaye senze uhlalutyo lokuphucula oluhambelanayo, sinike ulwazi kwiiprojekthi ezininzi zophando.

Iinkcukacha zeNkonzo

Ithala leeNcwadi

Icebo Lokulandelelanisa

Imveliso yedatha ecetyiswayo

Isisombululo seSignali ye-Hi-C

Ithala leencwadi le-Hi-C

I-Illumina PE150

Iluphu yeChromatin: 150x

I-TAD: 50x

I-Chromatin Loop: 10Kb

I-TAD: 40Kb

Iimfuno zeNkonzo

Uhlobo lwesampulu	Isixa esifunekayo
Izicubu zezilwanyana	≥2g
Igazi Elipheleleyo	≥2mL
Iifungi	≥1g
Izicubu ezincinci zezityalo	I-1g/aliquot, ii-aliquotes ezi-2-4 ziyacetyiswa
Iiseli ezikhuliswe kakuhle	≥1x10⁷

Ngaphambili: I-TGuide Smart Magnetic Plant DNA Kit

Okulandelayo: Iilayibrari ezenziwe kwangaphambili ze-DNBSEQ

Ibandakanya uHlahlelo Olulandelayo:

● Idatha engacwangciswanga QC;

● Imephu kunye ne-Hi-C library QC: ii-valid interaction pairs kunye ne-Interaction Decay Exponents (ii-IDE);

● Iprofayili yoNxibelelwano olupheleleyo lweGenome: uhlalutyo lwe-cis/trans kunye nemephu yonxibelelwano lwe-Hi-C;

● Uhlalutyo lokusasazwa kwe-A/B compartment;

● Ukuchongwa kwee-TAD kunye nee-chromatin loops;

● Uhlalutyo lokwahluka kwizinto zesakhiwo se-chromatin ze-3D phakathi kweesampuli kunye nenkcazo yokusebenza ehambelanayo yezakhi zofuzo ezinxulumene nazo.

Ukusasazwa kwe-Cis kunye ne-trans proportion

Imephu yobushushu yokusebenzisana kwe-chromosome phakathi kweesampuli

Ukusasazwa ngokubanzi kwe-genome kweendawo ze-A/B

Ukusasazwa ngokubanzi kwee-chromatin loops kwi-genome

Ukubonakala kwee-TAD

Hlola inkqubela phambili yophando eququzelelwe ziinkonzo zokulandelelana ze-BMKGene ze-Hi-C ngokusebenzisa ingqokelela yeempapasho ezikhethiweyo.

UMeng, T. et al. (2021) 'Uhlalutyo oluhlanganisiweyo oluhlanganisiweyo lwee-multi-omics luchonga i-CA2 njengenjongo entsha ye-chordoma',I-Neuro-Oncology, 23(10), iphepha 1709-1722. doi: 10.1093/NEUONC/NOAB156.

UXu, L. et al. (2021) 'Ukuphazamiseka kwe-3D kunye nokulungiswa kwakhona kwe-genome kubonelela ngengqiqo kwi-pathogenesis ye-NAFLD ngokulandelelana kwe-Hi-C, i-Nanopore, kunye ne-RNA',I-Acta Pharmaceutica Sinica B, 11(10), iphepha 3150-3164. doi: 10.1016/J.APSB.2021.03.022.