mRNA-seq (NGS) – De novo

Ukulandelelana kwe-mRNA kuvumela ukuprofayilisha zonke ii-mRNA transcripts kwiiseli phantsi kweemeko ezithile kwaye bubuchwepheshe obusetyenziswa kakhulu kwiindawo ezahlukeneyo zophando. Umbhobho we-BMKCloud De novo mRNA-seq wenzelwe ukuhlalutya iilayibrari zokulandelelana ezicebileyo ze-poly-A xa kungekho genome yesalathiso ekhoyo. Umbhobho uqala ngolawulo lomgangatho, ulandelwe ngude novoukuhlanganiswa kwe-transcript kunye nokukhethwa kweseti ye-unigene. Uhlalutyo lwesakhiwo se-unigene luqikelela ulandelelwano lwekhowudi (CDS) kunye nokuphindaphinda kolandelelwano olulula (SSR). Emva koko, uhlalutyo lokubonakaliswa komahluko lufumana ii-genes ezichazwe ngokwahlukileyo (ii-DEG) phakathi kweemeko ezivavanyiweyo, kulandele i-functional analysis kunye nokwandiswa kwee-DEG ukuze kukhutshwe ulwazi lwebhayoloji.