I-Nanopore ende epheleleyo ye-transcriptome

Ukulandelelana kwe-Nanopore transcriptome yindlela enamandla yokulandelelanisa i-cDNA epheleleyo, ukuchonga ngokuchanekileyo kunye nokulinganisa ii-isoforms ze-transcript. I-BMKCloud Nanopore Full-Length Transcriptome Pipeline yenzelwe ukuhlalutya idatha ye-RNA-Seq eyenziwe kwiqonga le-Nanopore ngokuchasene ne-genome yereferensi ebhalwe kakuhle esemgangathweni ophezulu, inika uhlalutyo olusemgangathweni nolunobungakanani kwinqanaba le-gene kunye ne-transcript. Emva kolawulo lomgangatho, kufunyanwa ulandelelwano olupheleleyo lwe-non-chimeric (FLNC) kwaye ulandelelwano lwemvumelwano ludweliswe kwi-genome yereferensi ukususa ii-transcript ezingafunekiyo. Kule seti ye-transcript, ukubonakaliswa kuyalinganiswa kwaye ii-genes ezichazwe ngokwahlukileyo kwaye ii-transcripts ziyachongwa kwaye zichazwe ngokusebenzayo. I-pipeline ikwabandakanya uhlalutyo lwe-polyadenylation (APA), uhlalutyo lwe-splicing olungelinye, uhlalutyo olulula lwe-repeat (SSR), uqikelelo lwe-lncRNA kunye neethagethi ezihambelanayo, uqikelelo lwe-coding sequences (CDS), uhlalutyo losapho lwe-gene, uhlalutyo lwezinto ze-transcription, uqikelelo lwe-genes ezintsha kunye ne-functional annotation ye-transcripts.