Pacbio Ubude obupheleleyo be-transcriptome

I-PacBio Ulandelelwano lwe-Transcriptome yoBubude obuPheleleyo, i-Isoseq, yenza ukuba kuchongwe ngokuchanekileyo i-isoforms ebhaliweyo, ikhanyise enye i-polyadenylation kunye ne-splicing, kwaye ikhokelela kuhlalutyo oluchanekileyo lwe-gene expression. Umbhobho we-BMKCloud PacBio woBubude obugcweleyo be-Transcriptome yenzelwe ukuhlalutya amathala eencwadi e-cDNA alandelelaniswe kwimowudi yeSetyhula ye-Consensus Sequencing (CCS) kwaye ichonge ulandelelwano loBubude obuBupheleleyo obungeyo-Chimeric (FLNC), abathi emva koko badityaniswe kwimibhalo engafunekiyo. Uhlalutyo olulandelayo lwe-BUSCO luvavanya ukugqibelela kwendibano ye-transcriptome. Ukusuka kwi-transcriptome edibeneyo, uhlalutyo oluninzi lwenziwa: ukuchaswa okungezinye, ukuphindaphinda okulandelelanayo (SSR), ukubikezelwa kwe-lncRNA kunye neejene ezijoliswe kuzo ezihambelanayo, ukuqikelelwa kweejene ezintsha, uhlalutyo lwentsapho ye-gene, uhlalutyo lwezinto ezibhaliweyo, kunye nokuchazwa okusebenzayo kokubhaliweyo.