I-transcriptome epheleleyo yePacbio

I-PacBio Full-Length Transcriptome Sequencing, i-Isoseq, ivumela ukuchongwa ngokuchanekileyo kwe-isoforms ze-transcript, ikhanyisela i-polyadenylation kunye ne-splicing eyahlukileyo, kwaye ikhokelele kuhlalutyo oluchanekileyo ngakumbi lwe-gene expression. Umbhobho we-BMKCloud PacBio Full-Length Transcriptome wenzelwe ukuhlalutya iilayibrari ze-cDNA ezilandelelanayo kwi-Circular Consensus Sequencing (CCS) mode kwaye ichonge i-Full-Length Non-Chimeric (FLNC) sequences, ezithi emva koko zihlanganiswe zibe yi-non-redundant transcripts. Uhlalutyo olulandelayo lwe-BUSCO luvavanya ukugqibelela kwe-transcriptome assembly. Ukusuka kwi-transcriptome edibeneyo, kwenziwa uhlalutyo oluninzi: i-alternative splicing, i-simple sequence repeat (SSR), i-prediction ye-lncRNA kunye ne-target target genes ehambelanayo, i-prediction ye-new genes, i-gene family analysis, i-transcription factor analysis, kunye ne-functional annotation ye-transcripts.