Iimveliso

Ulandelelwano lweGenome yeSityalo/yeSilwanyana

Isityalo/Isilwanyana Esipheleleyo Ulandelelwano lweGenome Umfanekiso obonakalayo

Ukulandelelana kwakhona kwe-genome yonke, eyaziwa ngokuba yi-WGS, yenza ukuba kutyhilwe zombini iinguqulelo eziqhelekileyo kunye nezinqabileyo kuyo yonke i-genome kuquka i-Single Nucleotide Polymorphism (SNP), ukucinywa kokuFaka (InDel), ukwahluka koLwakhiwo (SV), kunye neCopy Number Variation (CNV). ).I-SVs zenza inxalenye enkulu yesiseko sokwahluka kune-SNP kwaye ibe nempembelelo enkulu kwi-genome, enempembelelo ebalulekileyo kwizinto eziphilayo.Ukuphinda kufundwe ixesha elide kuvumela ukuchongwa okuchane ngakumbi kwamaqhekeza amakhulu kunye nokwahluka okuntsokothileyo kuba ukufunda ixesha elide kwenza kube lula ukuwela ichromosomal ngaphaya kwemimandla entsonkothileyo efana nokuphindaphinda kwe-tandem, imimandla etyebileyo yeGC/AT, kunye nemimandla eguquguqukayo.

Iqonga: Illumina, PacBio, Nanopore

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Iziphumo zeDemo

Ushicilelo olukhoyo

1.Iinzuzo zeNkonzo

Amava abanzi kulandelelwano lwe-genome kwiintlobo ezingaphezu kwe-1000.

Ngaphezulu kwamatyala angama-800 apapashiweyo anempembelelo eyongezelekayo engaphezu kwama-4000.

Uhlalutyo olubanzi lwe-bioinformatics kubizo lokwahluka kunye nohlalutyo lomsebenzi.

2. IiNgcaciso zeNkonzo

Iqonga		Ithala leencwadi	Ubunzulu be-seq obucetyiswayo
Illumina		PE 150	Nge-SNP, i-InDel ifowuna ≥ 10x Kwi-SV, i-CNY ibiza ≥ 30x
Nanopore		8kb	Kwi-SV, i-CNY ibiza ≥ 20x
Pacbio	CCS	15 kb	I-SNP, i-InDel, i-SV, i-CNY ibiza ≥ 10x

3. IiMfuno zeSampulu

Iqonga		Conc.(ng/μL)	Imali (ng)	Ubunyulu		Ijeli yeAgarose
				OD260/280	OD260/230	1. Sula ibhendi ephambili ngaphandle okanye elinganiselweukuthotywa okubonwe kwijeli. 2. Akukho okanye i-RNA encinci okanye i-protein contamination
Illumina		≥1	≥30	-	-
Nanopore		≥30	Kuxhomekeke kwimveliso yedatha 10μg/iseli	1.7-2.2	≥1.5
Pacbio	CCS	≥50		1.7-2.2	1.8-2.5

4. Uhlalutyo lwe-Bioinformation

5. UkuHamba komsebenzi weNkonzo

Ukuhanjiswa kwesampuli

Ukukhutshwa kwe-DNA

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Uhlalutyo lwedatha

Ukuhanjiswa kwedatha

Ngaphambili: I-BMKMANU S1000 i-Spatial Transcriptome

Okulandelayo: I-nucleus ye-RNA enye

1) Iinkcukacha-manani zeGenome Mapping

Uluhlu loku-1 lweziphumo zemephu

Umzobo 1 Ukuhanjiswa kobungakanani bokufaka kunye nokufunda ukugubungela.

2) Ukwahlula ukufunyanwa

Umzobo we-2 Izibalo kunye nenkcazo ye-SNP / INDEL / SV phakathi kweeSampuli

Umzobo 3 Ukuhanjiswa kweGenome ngokubanzi kweeatiations

3) Inkcazo esebenzayo yeenguqu

2019	Unxibelelwano lwendalo	Ukulandelelana kwe-genome yonke kubonisa imvelaphi ye-Brassica napus kunye ne-genetic loci ebandakanyekayo ekuphuculeni kwayo
2020	PNAS	Imvelaphi yendalo kunye nembali yokwenziwa kwasekhaya kwentlanzi yegolide (iCarassius auratus)
2021	Ijenali yeBiyoteknoloji yeZityalo	Iigenome zezalathisi zeentlobo ezimbini ezilinywayo zejute
2021	Ijenali yeBiyoteknoloji yeZityalo	Isiginitsha yeGenomic yemifuno kunye ne-oyile allopolyploid Brassicajuncea kunye ne-genetic loci elawula ukuqokelelwa kweglucosinolates
2019	Isityalo seemolekyuli	Ukulandelelana kwegenome ngokupheleleyo kwingqokelela yehlabathi jikelele ye-rapeseed accessions ityhila isiseko sofuzo sokwahlukana kwe-ecotype yabo
2022	Uphando lweHorticulture	Uhlalutyo lwe-genome-wide-wide association lubonelela ngengqiqo yeemolekyuli kwinguqu yendalo yobungakanani bembewu yevatala
2021	Ijenali yoMfuniselo wezityalo	Uphononongo lomanyano olubanzi lweGenome luchonga ukwahluka kwe-GhSAD1 enika unyamezelo olubandayo kumqhaphu
2021	Ijenali yoMfuniselo wezityalo	Uphononongo olubanzi lwe-Genome-wide association kunye nothelekiso lwe-transcriptome lutyhila inoveli ye-QTL kunye nofuzo lomviwa olawula ubungakanani bepetal kwi-rapeseed.