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PacBio-Full-length 16S/18S/ITS Amplicon Sequencing
Iqonga le-Amplicon (16S / 18S / ITS) liphuhliswa kunye neminyaka yamava kuhlalutyo lweprojekthi ye-microbial diversity, equlethe uhlalutyo olusisiseko olusemgangathweni kunye nohlalutyo lomntu siqu: uhlalutyo olusisiseko lubandakanya umxholo wohlalutyo oluqhelekileyo lophando lwangoku lwe-microbial, umxholo wokuhlalutya ucebile kwaye ubanzi, kunye neziphumo zohlalutyo zinikezelwe ngohlobo lweengxelo zeprojekthi; Umxholo wohlalutyo lobuqu uhlukile. Iisampulu zinokukhethwa kwaye iiparameters zinokumiselwa ngokuguquguqukayo ngokwengxelo yohlalutyo olusisiseko kunye nenjongo yophando, ukufezekisa iimfuno zomntu. Inkqubo yokusebenza yeWindows, ilula kwaye iyakhawuleza.
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I-PacBio-ubude obugcweleyo be-Transcriptome (Non-Reference)
Ukuthatha i-Pacific Biosciences (i-PacBio) ye-Isoform yokulandelelanisa idatha njengegalelo, le App iyakwazi ukuchonga ulandelelwano olupheleleyo lwe-transcript (ngaphandle kwendibano). Ngokwenza imephu yolandelelwano lobude obupheleleyo ngokuchasene ne-genome yereferensi, imibhalo inokuthi iphuculwe ngejene eyaziwayo, imibhalo ebhaliweyo, imimandla yekhowudi, njl.njl. Kule meko, ukuchongwa okuchaneke ngakumbi kwezakhiwo ze-mRNA, njengokunye ukudityaniswa, njl njl, kunokufezekiswa. Uhlalutyo oludityanisiweyo kunye nedatha ye-NGS yolandelelwano lwe-transcriptome yenza ukuba inkcazo ebanzi ngakumbi kunye nobungakanani obuchanekileyo ekubonakalisweni kwinqanaba le-transcript, elizuza kakhulu kwintetho ephantsi yolwahlulo kunye nohlalutyo olusebenzayo.
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Iikhithi zezixhobo
I-BMKCloud yiqonga elikhokelayo le-bioinformatic elibonelela ngesisombululo esisodwa kwiinkqubo ze-genomic, ezithenjwa ngokubanzi ngabaphandi kwiindawo ezahlukeneyo ezibandakanya ezonyango, ezolimo, ezendalo, njl. , izixhobo zekhompyutha, isiseko sedatha yoluntu, izifundo ze-intanethi ye-bioinformatic, njl. njl. I-ncRNA, ulawulo lomgangatho wedatha, ukudibanisa, ulungelelwaniso, ukutsalwa kwedatha, utshintsho, izibalo, umvelisi wemifanekiso, uhlalutyo lolandelelwano, njl.
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iRNA encinci
Ii-RNA ezincinci luhlobo lwe-RNA emfutshane engeyiyo ikhowudi kunye nobude obuphakathi kwe-18-30 nt, kuquka i-miRNA, i-siRNA kunye ne-piRNA. Ezi RNA zincinci ziye zaxelwa kakhulu ukuba zibandakanyeka kwiinkqubo ezahlukeneyo zebhayoloji ezifana nokuthotywa kwe-mRNA, inhibition yokuguqulela, ukubunjwa kwe-heterochromatin, njl njl. Uhlalutyo lokulandelelana kwe-SmallRNA lusetyenziswe ngokubanzi kwizifundo zokuphuhliswa kwezilwanyana / izityalo, izifo, intsholongwane, njl njl. iqonga lokuhlalutya ulandelelwano lubandakanya uhlalutyo olusemgangathweni kunye nokumbiwa kwedatha ephezulu. Kwisiseko sedatha ye-RNA-seq, uhlalutyo olusemgangathweni lunokufikelela ukuchongwa kunye noqikelelo lwe-miRNA, uqikelelo lwemfuza ekujoliswe kuyo yi-miRNA, inkcazo kunye nohlalutyo lwentetho. Uhlalutyo oluphezulu luvumela ukukhangela kunye nokutsalwa kwe-miRNA ngokwezifiso, ukuveliswa komzobo weVenn, i-miRNA kunye nolwakhiwo lwenethiwekhi yemfuza ekujoliswe kuyo.
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I-NGS-WGS (Illumina/BGI)
I-NGS-WGS liqonga lokuhlalutya lokulandelelana kwakhona kwe-genome, eliphuhliswe kwisiseko samava atyebileyo kwi-Biomarker Technologies. Eli qonga lisebenziseka lula livumela ukungeniswa okukhawulezayo kokuhamba komsebenzi wokuhlalutya okudibeneyo ngokubeka nje iparameter embalwa esisiseko, ehambelana nedatha yolandelelwano yeDNA eyenziwe kwiqonga le-Illumina kunye neqonga lokulandelelana kwe-BGI. Eli qonga libekwe kwiseva ephezulu yokusebenza kwekhompyuter, exhobisa uhlalutyo lwedatha olusebenzayo ngexesha elincinci kakhulu. Ukumbiwa kwedatha yobuqu kufumaneka kwisiseko sohlalutyo olusemgangathweni, kubandakanywa nombuzo oguquliweyo we-gene, uyilo lwe-PCR primer, njl.
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mRNA(Reference)
I-Transcriptome likhonkco phakathi kolwazi lwemfuzo ye-genomic kunye neproteome yomsebenzi webhayoloji. Ummiselo wenqanaba lokukhutshelwa ngowona ubalulekileyo kwaye yeyona ndlela yolawulo efundwa ngokubanzi yezinto eziphilayo. Ulandelelwano lwe-Transcriptome luyakwazi ukulandelelanisa i-transcriptome nakweyiphi na indawo ngexesha okanye phantsi kwayo nayiphi na imeko, kunye nesisombululo esichanekileyo kwi-nucleotide enye. isampula yemibhalo ebhaliweyo ethile.
Okwangoku, itekhnoloji yokulandelelanisa i-transcriptome isetyenziswe ngokubanzi kwi-agronomy, iyeza kunye nezinye iindawo zophando, kubandakanywa ukulawulwa kokuphuhliswa kwezilwanyana kunye nezityalo, ukulungelelaniswa kwendalo, ukusebenzisana kwe-immune, i-gene localization, iintlobo ze-genetic evolution kunye ne-tumor kunye nokufumanisa isifo sofuzo.
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IMetagenomics (NGS)
Eli qonga lokuhlalutya lenzelwe ukudubula kwedatha ye-metagenomic kwisiseko seminyaka yamava. Iquka ukuhamba komsebenzi okudityanisiweyo okuqulethe uhlalutyo lweemetagenomics ezahlukeneyo ezifunekayo ngokuqhelekileyo kubandakanywa ukusetyenzwa kwedatha, izifundo zenqanaba leentlobo, izifundo ze-gene function-level studies, i-metagenome binning, njl. Ukongeza, izixhobo zokumbiwa kwedatha ezilungiselelwe ziyafumaneka ekuhlalutyweni okusemgangathweni komsebenzi, kubandakanywa nofuzo kunye nombuzo weentlobo. , ukusetwa kweparamitha, ukuvelisa amanani omntu, njl.
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LncRNA
Ii-RNA ezinde ezingezizo iikhowudi (lncRNA) luhlobo lwemibhalo enobude obude kune-200 nt, engakwaziyo ukufaka iiprotheyini zekhowudi. Ubungqina obuninzi bucebisa ukuba uninzi lwee-lncRNAs kusenokwenzeka ukuba zisebenze. Itekhnoloji yokulandelelaniswa komgangatho ophezulu kunye nezixhobo zokuhlalutya i-bioinformatic zisixhobisa ukuba siveze ulandelelwano lwe-lncRNA kunye nokubeka ulwazi ngokufanelekileyo kwaye sikhokelele ekufumaneni ii-lncRNAs ezinemisebenzi ebalulekileyo yokulawula. I-BMKCloud inebhongo ngokubonelela abathengi bethu ngeqonga lokuhlalutya lolandelelwano lwe-lncRNA ukuphumeza uhlalutyo olukhawulezayo, oluthembekileyo noluguquguqukayo lwe-lncRNA.
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I-GWAS
I-Genome-wide association study (GWAS) ijolise ekuchongeni ukuhlukahluka kofuzo (i-genotype) ehambelana neempawu ezithile (phenotype). Izifundo ze-GWA ziphanda iimpawu zofuzo ezinqamlezileyo zegenome yenani elikhulu labantu kwaye ziqikelela unxulumano lwe-genotype-phenotype ngohlalutyo lwamanani kumgangatho wabemi. Ulandelelwano lwejini yemfuza yonke lunakho ukufumana zonke iintlobo ezahlukeneyo zemfuza. Ukudibanisa kunye nedatha ye-phenotypic, i-GWAS inokucutshungulwa ukuchonga i-phenotype ehambelana ne-SNP, ii-QTL kunye nejene lomgqatswa, elixhasa ngamandla ukuzala kwezilwanyana / izityalo zanamhlanje. I-SLAF sisicwangciso esenziwe lula sokulandelelana kwe-genome, efumanisa iimpawu ezisasazwe ngokubanzi ze-genome, i-SNP. Ezi SNP, njengabamakishi be-molecular genetic, banokucutshungulwa kwizifundo zombutho kunye neempawu ezijoliswe kuzo. Sisicwangciso esingabizi kakhulu ekuchongeni iimpawu ezintsonkothileyo ezinxulumene nokwahluka kofuzo.
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I-Nanopore Ubude obupheleleyo be-transcriptomics
Ii-isoforms ezintsonkothileyo neziguquguqukayo kwizinto eziphilayo ziindlela ezibalulekileyo zofuzo zokulawula ukubonakaliswa kofuzo kunye nokwahluka kweeprotheyini. Ukuchongwa ngokuchanekileyo kwezakhiwo ze-transcript sisiseko sophononongo olunzulu lweendlela zokulawula i-gene expression. Iqonga lolandelelwano lweNanopore lizise ngempumelelo uphononongo lwe-transcriptomic kumgangatho we-isoform. Eli qonga lokuhlalutya lenzelwe ukuhlalutya idatha ye-RNA-Seq eveliswe kwiqonga leNanopore kwisiseko se-genome yereferensi, efezekisa uhlalutyo olunobulunga kunye nobungakanani kwinqanaba le-gene kunye nenqanaba lokubhaliweyo.
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malunga ne-RNA
I-RNA yeSetyhula (i-circRNA) luhlobo lwe-RNA engeyiyo ikhowudi, efunyenwe kutshanje ukuba idlala indima ebalulekileyo kuthungelwano olulawulayo olubandakanyekayo ekuphuhliseni, ukuchasana nokusingqongileyo, njl njl. Ukwahluke kwiimolekyuli ze-RNA ezinomgca, umzekelo, i-mRNA, i-lncRNA, i-3 kunye ne-5 Iziphelo ze-circRNA zidityaniswe kunye ukwenza ulwakhiwo olusetyhula, olubasindisa ekugayweni kwe-exonuclease kwaye zizinze ngakumbi kunobuninzi be-exonuclease. umgca weRNA. I-CircRNA ifunyenwe inemisebenzi eyahlukeneyo yokulawula ukubonakaliswa kofuzo. I-CircRNA inokusebenza njenge-ceRNA, ebopha i-miRNA ngokhuphiswano, eyaziwa ngokuba yi-miRNA sponge. Iqonga lokuhlalutya lokulandelelana kwe-CircRNA lixhobisa ubume be-circRNA kunye nohlalutyo lwentetho, ukuqikelela okujoliswe kuko kunye nohlalutyo oludibeneyo kunye nezinye iintlobo zeemolekyuli ze-RNA.
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BSA
Iqonga le-Bulked Segregant Analysis iquka uhlalutyo olusemgangathweni lwenyathelo elinye kunye nohlalutyo oluphambili kunye nokumisela ipharamitha elungiselelwe. I-BSA bubuchule obusetyenziselwa ukuchonga ngokukhawuleza iimpawu zemfuzo ezinxulumene nephenotype. Umsebenzi ongundoqo we-BSA uqulathe: 1. ukukhetha amaqela amabini abantu abaneephenotypes ezichasene kakhulu; 2. ukudibanisa i-DNA, i-RNA okanye i-SLAF-seq(Iphuhliswe yi-Biomarker) yabo bonke abantu ukuze benze isambuku esibini se-DNA; 3. ukuchonga ulandelelwano lokwahlula ngokuchasene ne-reference genome okanye phakathi, 4. ukuqikelela umviwa onxibelelene nemimandla nge-ED kunye ne-SNP-index algorithm; 5. Ucazululo olusebenzayo kunye nophuculo kwiijini kwimimandla yabaviwa, njl.njl. Ukumbiwa kwemigodi okuqhubele phambili ngakumbi kwidatha kubandakanywa ukuskrinwa kwe-genetic marker kunye ne-primer design kukwakhona.
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I-Amplicon (16S/18S/ITS)
Iqonga le-Amplicon (16S / 18S / ITS) liphuhliswa kunye neminyaka yamava kuhlalutyo lweprojekthi ye-microbial diversity, equlethe uhlalutyo olusisiseko olusemgangathweni kunye nohlalutyo lomntu siqu: uhlalutyo olusisiseko lubandakanya umxholo wohlalutyo oluqhelekileyo lophando lwangoku lwe-microbial, umxholo wokuhlalutya ucebile kwaye ubanzi, kunye neziphumo zohlalutyo zinikezelwe ngohlobo lweengxelo zeprojekthi; Umxholo wohlalutyo lobuqu uhlukile. Iisampulu zinokukhethwa kwaye iiparameters zinokumiselwa ngokuguquguqukayo ngokwengxelo yohlalutyo olusisiseko kunye nenjongo yophando, ukufezekisa iimfuno zomntu. Inkqubo yokusebenza yeWindows, ilula kwaye iyakhawuleza.
