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Iiproteomics
I-Proteomics igxile kwiiproteni—abaphathi bemisebenzi yobomi edlala indima ebalulekileyo kulawulo lokubhalwa kwezinto eziphilayo. Ihlalutya ukwakheka, amanqanaba okubonakaliswa, kunye neemeko zokuguqulwa kwazo zonke iiproteni ezitshintsha ngokuguquguqukayo kwizicwili okanye kwiiseli, ijongana nempembelelo ebalulekileyo ye-proteome abubuninzi be-dynamics kwiinkqubo ezahlukeneyo zobomi. Isetyenziswa kakhulu kumayeza, kwezolimo, nakwimfuyo. I-Qualitative Proteomics isebenzisa iteknoloji yokuchonga iiproteni ze-HPLC-MS/MS ukuchonga iisampuli kuquka imicu yejeli, i-IP, kunye neesampuli ze-CO-IP/Pull down. I-Quantitative Proteomics ifezekisa ukulinganiswa okuchanekileyo kunye nokuchongwa kwazo zonke iiproteni ezivezwa yi-genome okanye kwinkqubo exutyiweyo eyinkimbinkimbi. Iitekhnoloji ze-quantitative proteomics zangoku zihlulwe ngokubanzi kwiindlela ezibhalwe ilebhile (TMT) kunye nezingabhalwanga ilebhile (ezingenalebhile, i-DIA, i-PRM). I-BMKGENE ibonelela ngezisombululo ze-proteomics ezinamaqonga amaninzi kunye netekhnoloji eninzi.
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I-Metabolomics
I-Metabolomics, isifundo esisezantsi se-genomics, ikakhulu ijolise kwizinto ezincinci ze-molecule ezinobunzima be-molecular obungaphantsi kwe-1500 Da. Ivumela ii-metabolites ukuba zibonise iimpendulo zezinto eziphilayo kwi-stimuli zangaphandle kunye notshintsho lwe-physiological/pathological ngononophelo ngakumbi. Utshintsho lwe-genetic-olubangelwa kukutshintsha kwamanqanaba e-metabolite nalo lukwindawo yalo yophando, lubonelela ngembono entsha yophando.
I-BMKGENE inikezela ngoluhlu olupheleleyo lweenkonzo ze-metabolomics, kuquka i-metabolomics engajoliswanga, i-metabolomics ejoliswa kakhulu, kunye ne-metabolomics ejoliswayo. Ukusebenzisa i-liquid chromatography-mass spectrometry (LC-MS) okanye i-gas chromatography-mass spectrometry (GC-MS), utshintsho olutshintshayo kwii-metabolites ezininzi ze-molecule ezincinci kwizinto eziphilayo ngaphambi nasemva kokukhuthazwa kwangaphandle kunokubonwa. Umongo wezi nkonzo usekwe ekuchongeni ii-metabolites ezinomahluko omkhulu phakathi kwamaqela ovavanyo kunye namaqela olawulo kunye nokuhlola ngakumbi ulwalamano lwazo notshintsho lwe-physiological/pathological kunye neendlela ezisisiseko.
